The present application discloses a flowcell transfer device, a station layout device, and a sequencing system. The flowcell transfer device includes: a frame; a multi-dimensional motion mechanism arranged on the frame; and a flowcell gripping mechanism arranged on the multi-dimensional motion mechanism. The flowcell gripping mechanism includes: a first gripper driving member arranged on the multi-dimensional motion mechanism; and a first gripper connected to the first gripper driving member, where the first gripper includes oppositely arranged first jaw and second jaw, and driven by the first gripper driving member, the first jaw and the second jaw move toward or away from each other to grip or place the flowcell. The flowcell transfer device enables sequencing of multiple flowcells at multiple stations, which can effectively improve sequencing efficiency. The flowcell gripping mechanism can reliably grip or place the flowcell, and no additional design is required for the flowcell. The structure is simple, and the cost is low.