Methods of preparing a functionalized nanofiber preparing a dispersion of a polymer and a water soluble synthetic construct of the structure F-S-L in admixture in a liquid medium; and then electrospinning the polymer from the liquid medium to provide the functionalized nanofiber. F is the functional moiety, L is a lipid and S is a spacer linking F to L via covalent bonds.

A method for preparing placenta membrane tissue grafts for medical use, includes obtaining a placenta from a subject, cleaning the placenta, separating the chorion tissue from the amniotic membrane, mounting a selected layer of either the chorion tissue or the amniotic membrane onto a drying fixture, dehydrating the selected layer on the drying fixture, and cutting the selected layer into a plurality of tissue grafts. Preferably, the drying fixture includes grooves or raised edges that define the outer contours of each desired tissue graft, after they are cut, and further includes raised or indented logos that emboss the middle area of the tissue grafts during dehydration and that enables an end user to distinguish the top from the bottom side of the graft. The grafts are comprised of single layers of amnion or chorion, multiple layers of amnion or chorion, or multiple layers of a combination of amnion and chorion.

A method for preparing placenta membrane tissue grafts for medical use, includes obtaining a placenta from a subject, cleaning the placenta, separating the chorion tissue from the amniotic membrane, mounting a selected layer of either the chorion tissue or the amniotic membrane onto a drying fixture, dehydrating the selected layer on the drying fixture, and cutting the selected layer into a plurality of tissue grafts. Preferably, the drying fixture includes grooves or raised edges that define the outer contours of each desired tissue graft, after they are cut, and further includes raised or indented logos that emboss the middle area of the tissue grafts during dehydration and that enables an end user to distinguish the top from the bottom side of the graft. The grafts are comprised of single layers of amnion or chorion, multiple layers of amnion or chorion, or multiple layers of a combination of amnion and chorion.

The present disclosure relates to a medical device and methods of making the same. The medical device includes a porous substrate and at least one film. The film is formed within the pore of the substrate. The film is intra-porous and does not contact adjacent pores or films.

Methods for the conditioning of bioprosthetic material employ bovine pericardial membrane. A laser directed at the fibrous surface of the membrane and moved relative thereto reduces the thickness of the membrane to a specific uniform thickness and smoothes the surface. The wavelength, power and pulse rate of the laser are selected which will smooth the fibrous surface as well as ablate the surface to the appropriate thickness. Alternatively, a dermatome is used to remove a layer of material from the fibrous surface of the membrane. Thinning may also employ compression. Stepwise compression with cross-linking to stabilize the membrane is used to avoid damaging the membrane through inelastic compression. Rather, the membrane is bound in the elastic compressed state through addition cross-linking. The foregoing several thinning techniques may be employed together to achieve strong thin membranes.

A method for preparing placenta membrane tissue grafts for medical use, includes obtaining a placenta from a subject, cleaning the placenta, separating the chorion tissue from the amniotic membrane, mounting a selected layer of either the chorion tissue or the amniotic membrane onto a drying fixture, dehydrating the selected layer on the drying fixture, and cutting the selected layer into a plurality of tissue grafts. Preferably, the drying fixture includes grooves or raised edges that define the outer contours of each desired tissue graft, after they are cut, and further includes raised or indented logos that emboss the middle area of the tissue grafts during dehydration and that enables an end user to distinguish the top from the bottom side of the graft. The grafts are comprised of single layers of amnion or chorion, multiple layers of amnion or chorion, or multiple layers of a combination of amnion and chorion.

The present invention relates to a device for the administration of substances onto the epicardial surface of the heart. The device comprises a frame structure which is able to assume shaping, positioning, guiding and stabilizing functions. The frame structure may contain at least one sleeve. The device comprises a substance carrier which is able to accommodate the substance to be administered.

Polymeric articles including fibers, films, and solid surfaces may be produced with improved properties by embedding particles within the article. The particles may be any particle that imparts desired properties to the fiber, film, or solid surface. Methods of selecting the particle size based upon the desired properties of the article such as the fiber diameter, film thickness, efficacy such as antimicrobial activity of the article, and/or article compositions are also described. For example, fibers having improved antimicrobial, antiviral, and antifungal properties are described. The articles having antimicrobial, antiviral, and antifungal properties may comprise a plurality of particles comprising water insoluble copper compounds that release at least one of Cu+ ions and Cu++ ions upon contact with a fluid. The average particle size or the particle size range is selected based upon the diameter of the fiber or thickness of the film to be produced.

The present invention relates to a bioactive compound delivery assembly, a method for stabilization and/or encapsulation of bioactive compound compositions, a method for solid-supported transfection of living cells as well as a use of the bioactive compound delivery assembly.

A catheter insert device includes a powder composition, and a housing. The powder composition includes a solid phase S-nitrosothiol (RSNO). The housing includes a polymeric wall that is i) permeable to nitric oxide, ii) non-porous, and iii) permeable to water vapor, and an inner lumen defined at least in part by the polymeric wall. The powder composition is completely sealed within the inner lumen of the housing.