The present invention relate to three dimensional porous polysaccharide matrices able to induce mineralisation of a tissue in osseous site, as well as in non-osseous site, in the absence of stent cells or growth factors.

A biocompatible membrane composite that can provide an environment that is able to mitigate or tailor the foreign body response is provided. The membrane composite contains a mitigation layer and a vascularization layer. A reinforcing component may optionally be included to provide support to and prevent distortion of the biocompatible membrane composite in vivo. The mitigation layer may be bonded (e.g., point bonded or welded) or adhered (intimately or discretely) to an implantable device and/or cell system. The biocompatible membrane composite may be used as a surface layer for implantable devices or cell systems that require vascularization for function but need protection from the host's immune response, such as the formation of foreign body giant cells. The biocompatible membrane composite may partially or fully cover the exterior of an implantable device or cell system. The mitigation layer is positioned between the implantable device or bioactive scaffold and the vascularization layer.

Methods for making an implant scaffold, comprising providing a 3D template generated according to an image of a lesion site, contacting the 3D template with a solution comprising a polymeric precursor, and evaporating the solution, thereby obtaining an implant scaffold, are provided. Further, implant scaffolds, comprising a water-soluble template in the form of a 3D geometrical array and a polymeric material are provided.

A system for treating open wounds includes a foam material configured for pouring, spraying, injecting or spreading on a wound bed. The foam material can comprise a base component and a curing component, which can be pre-mixed before application, or mixed in situ as the components are being applied to the wound site. A third component can comprise a sacrificial porogen. Foam can be placed in the wound bed as a wound liner on the wound surfaces. An additional foam insulation can provide a foam filler partially contained by the wound liner and generally flush with a patient's epidermis. A method of treating open wounds includes the steps of applying the wound liner and filler components. An optional step comprises covering the wound liner with a semi-permeable (breathable) membrane and mounting inlet and outlet ports thereon for introducing healing compositions as input, and extracting wound exudates as output.

In some embodiments, the present invention provides methods for making resorbable ear tubes including the steps of providing a silk fibroin solution, and forming a silk ear tube from the silk fibroin solution, wherein the silk ear tube is less than 2 mm in length and has an outer diameter of less than 1.5 mm, and wherein the silk ear tube is resorbable. In some embodiments, the present invention also provides methods for treating otitis media including the step of introducing a silk ear tube into the ear canal of a subject, wherein the silk ear tube is less than 2 mm in length and has an outer diameter of less than 1.5 mm, and wherein the silk ear tube is resorbed by the subject.

The present invention relates to a process for the preparation of a porous hierarchical scaffold characterized by internal pores organized according to a hierarchical structure, for the regeneration, replacement and/or simulation of a bone tissue, in particular of the trabecular and/or cortical bone tissue. The present invention further relates to the porous hierarchical scaffold obtainable by such process and to the uses thereof. The invention further relates to the use of a sacrificial hierarchical scaffold for the preparation of such porous hierarchical scaffold.

A method and device for fabricating vascular networks in for tissue engineering. The vascular network is embedded in a porous scaffold and is created from a sacrificial wax template, according to one embodiment. A extrusion-based three dimensional printer is used to create the template, wherein the printer utilizes an extruder incorporating a mixer to maintain the consistency of the extrudate.

The present invention provides porous biomimetic scaffolds and methods for making the same. The scaffolds have graded pore sizes for enhanced cell penetration. The scaffolds are useful for wound regeneration by facilitating cell penetration into the scaffold interior and due to their inherent immunomodulatory effects. The scaffolds have tissue modeling specification by mimicking the inherent stratified structure of certain tissues.

Provided herein are methods for making foam materials and foam material products having a polyurethane foam matrix defining a plurality of pores, a hydrophilic agent retained within at least a portion of the pores for improving an absorption of the foam material, a salt retained within at least a portion of the pores in an amount sufficient to render the foam material isotonic, a surfactant retained within at least a portion of the pores in an amount sufficient to be released upon contact with a moist surface. Also provided herein are methods for making a multilayer foam by casting a second foam layer on a first foam layer substrate and compressing the second foam layer before the second layer is fully cured to form an interface layer in situ.

Provided herein are composite scaffold biomaterials including two or more scaffold biomaterial subunits, each including a decellularized plant or fungal tissue from which cellular materials and nucleic acids of the tissue are removed, the decellularized plant or fungal tissue having a 3-dimensional porous structure, the two or more scaffold biomaterial subunits being assembled into the composite scaffold biomaterial and held together via gel casting using a hydrogel glue; via complementary interlocking geometry of the two or more scaffold biomaterial subunits; via guided assembly based biolithography (GAB); via chemical cross-linking; or any combinations thereof. Methods for producing such scaffold biomaterials, as well as methods and uses thereof, are also provided.