A system and method for producing hair follicle stem cell 3D organoid using a feeder cell or cell line where the feeder cell or cell line is a dermal endothelial cell, a fibroblast cell or a cell line that is similar to the target cell or the same type of the target cell. The system and method provide rapid culture as well as a long-term sustainable 3D cell or tissue culture environment and also a treatment for hair loss.

A system and method for producing 3D organoid using a feeder cell or cell line where the feeder cell or cell line is an endothelial cell, a fibroblast cell or a cell line that is similar to the target cell or the same type of the target cell. The system and method provide rapid culture as well as a long-term sustainable 3D cell or tissue culture environment.

The present invention provides a method for manufacturing a regular and high-density regenerated hair follicle primordium aggregation similar to the hair follicle tissue of a mammal in a simple manner. A regenerated hair follicle primordium aggregation manufacturing method of the present invention includes a step of forming hair follicle primordia by inoculating a microwell plate, which includes regularly arranged microwell portions, with mesenchymal cells and epithelial cells and culturing a mixture of the cells while supplying oxygen thereto.

A suturing needle is provided for injecting a gold thread. The suturing needle includes a needle body having a sharpened tip part at an upper end thereof. In addition, the suturing needle includes an upper groove provided on the sharpened tip part to hold the gold thread therein, and a lower groove provided near a lower end of the needle body to be opposite to the upper groove. Also, the suturing needle includes a handle part detachably combined with the lower end of the needle body. Various embodiments are possible.

A suturing needle is provided for injecting a gold thread. The suturing needle includes a needle body having a sharpened tip part at an upper end thereof. In addition, the suturing needle includes an upper groove provided on the sharpened tip part to hold the gold thread therein, and a lower groove provided near a lower end of the needle body to be opposite to the upper groove. Also, the suturing needle includes a handle part detachably combined with the lower end of the needle body. Various embodiments are possible.

Compositions containing purified collagen biomaterial derived from tissues, for example, insoluble amnion, soluble amnion, soluble chorion of the human placenta, are provided. The collagen compositions can be used to promote wound healing, promote tissue regeneration, prevent or reduce scarring, reduce local inflammation, minimize tissue rejection, promote graft integration. Methods for using the collagen composition as a biomaterial implant for dermal filling, skin grafting, and hair transplantation are also provided.

Compositions containing purified collagen biomaterial derived from tissues, for example, insoluble amnion, soluble amnion, soluble chorion of the human placenta, are provided. The collagen compositions can be used to promote wound healing, promote tissue regeneration, prevent or reduce scarring, reduce local inflammation, minimize tissue rejection, promote graft integration. Methods for using the collagen composition as a biomaterial implant for dermal filling, skin grafting, and hair transplantation are also provided.

Disclosed herein is use of collagen particles for preparation of a medicament for inducing hair follicles neogenesis or angiogenesis in a subject. The collagen particles in the present application have a diameter of about 10-200 ?m. According to some embodiments of the present disclosure, the collagen particles are administered to the subject in an amount of about 0.1 mg/cm.sup.2 to about 1,000 mg/cm.sup.2.

Provided herein are tissue implants and uses thereof. In certain aspects, tissue implants are described that can be used to help repair, rejuvenate, and/or revitalize the scalp. Also provided herein are methods of making, use, and administration thereof. The tissue implants can be prepared by harvesting cells or tissue from a donor and selectively lysing the cells or tissue to obtain the intracellular content. Also provided herein are delivery devices for delivering the tissue implants described herein and kits that include the tissue implants described herein.

Compositions and methods for producing reconstituted human skin and/or hair follicles in situ are provided. The method for producing the skin is unique in that tissue culture expanded cells including multipotent cells such as neonatal cells as well as cultured epidermal and dermal cells are immediately placed on a substrate such as a membrane and then the membrane with adherent cells is placed on a skin wound. Examples demonstrate formation of hair follicles in situ.