Provided herein are synthetic, three-dimensional (3D) bioprinted tissue constructs comprising porcine cells and methods of producing and using the same. The synthetic 3D bioprinted tissue constructs are fabricated by bioprinting spheroids comprising porcine cells, including genetically engineered cells, on a microneedle mold and fusing the spheroids to form an engineered tissue construct. Also provided are methods of using scaffold-free 3D bioprinted tissue constructs for applications related to drug screening and toxicity screening.

The present invention discloses an automated device for tissue and organ engineering. The device comprises a main chamber for performing decellularization, tissue sterilization, and recellularization and a set of solution and medium chambers, which provide solutions and medium for the decellularization and recellularization processes, respectively, in a continuous closed circuit. The device further comprises a sterilizing system for self-sterilization of the automated device. The device further comprises a user interface to input steps of protocols for tissue or organ engineering. The device further comprises a controller configured to control valves and pumps, which control and direct the flow of solutions and medium based on the steps of protocols, thereby automating the processes of decellularization, tissue sterilization, recellularization and self-sterilization. All parts of the device are installed in one single body in a fully integrated manner, in one example, which makes the device ready to use. The device minimizes the user intervention and enhances sterility, reproducibility, and efficiency.

A disposable device for absorbing urine and/or bodily fluids in the urethra having a cylindrical body having a top and bottom end. The body is made of absorbent material that expands upon contact with urine and bodily fluids and includes a string connected to the bottom end of the body for removing the device from the urethra. The device can include a vasodilator in or on a surface of the body and can be used to treat urinary incontinence and/or erectile dysfunction by inserting into the urethra and removing the device after it has been impregnated with fluid. The device can be included in a kit with a plunger for insertion.

The present disclosure is related to a perfusable-type bio-dual proximal tubule cell construct and a producing method thereof capable of applying an in vitro artificial organ model configured to include a first bioink comprising a decellularized substance derived from a mammalian kidney tissue and human umbilical vascular endothelial cells (HUVECs) and a second bioink comprising the decellularized substance and renal proximal tubular epithelial cells (RPTECs), wherein the first bioink and the second bioink are coaxial and printed in tubular constructs having different inner diameters.

According to the present disclosure, it is possible to use the renal proximal tubule-on-a-chip as a bioreactor capable of observing a biological drug reaction similar to a real drug by perfusing various drugs to the renal proximal tubule-on-a-chip.

A nephrospheroid comprising urine-derived epithelial cells, the nephrospheroid is capable of forming a tubular nephric tissue upon transplantation. Also provided are methods of producing the nephrospheroid and using same.

Compositions and methods of transplanting cells by grafting strategies into solid organs (especially internal organs) are provided. These methods and compositions can be used to repair diseased organs or to establish models of disease states in experimental hosts. The method involves attachment onto the surface of a tissue or organ, a patch graft, a “bandaid-like” covering, containing epithelial cells with supporting early lineage stage mesenchymal cells. The cells are incorporated into soft gel-forming biomaterials prepared under serum-free, defined conditions comprised of nutrients, lipids, vitamins, and regulatory signals that collectively support stemness of the donor cells. The graft is covered with a biodegradable, biocompatible, bioresorbable backing used to affix the graft to the target site. The cells in the graft migrate into and throughout the tissue such that within a couple of weeks they are uniformly dispersed within the recipient (host) tissue. The mechanisms by which engraftment and integration of donor cells into the organ or tissue involve multiple membrane-associated and secreted forms of MMPs.

Described are methods, cell growth substrates, and devices that are useful in preparing cell-containing graft materials for administration to patients. Tubular passages can be defined in cell growth substrates to promote distribution of cells into the substrates. Also described are methods and devices for preparing cell-seeded graft compositions, methods and devices for preconditioning cell growth substrates prior to application of cells, and cell seeded grafts having novel substrates, and uses thereof.

A method or apparatus for creating a three-dimensional tissue construct of a desired shape for repair or replacement of a portion of an organism. The method may comprise injecting at least one biomaterial in a three-dimensional pattern into a first material such that the at least one biomaterial is held in the desired shape of the tissue construct by the first material. The apparatus may comprise an injector configured to inject at least one biomaterial in a three-dimensional pattern into a first material such that the at least one biomaterial is held in the desired shape of the tissue construct by the first material. The first material may comprise a yield stress material, which may be a material exhibiting Herschel-Bulkley behavior. The tissue construct may have a smallest feature size of ten micrometers or less.

The method for decellularization of kidney tissue according to the present invention, the decellularized material produced through the method, and a bioink comprising the decellularized material have the effect of maximizing the effect of kidney treatment by maximizing the content of components specialized for kidney treatment such as the collecting duct and renal tubule of the kidney.

The present invention discloses an automated device for tissue and organ engineering. The device comprises a main chamber for performing decellularization, tissue sterilization, and recellularization and a set of solution and medium chambers, which provide solutions and medium for the decellularization and recellularization processes, respectively, in a continuous closed circuit. The device further comprises a sterilizing system for self-sterilization of the automated device. The device further comprises a user interface to input steps of protocols for tissue or organ engineering. The device further comprises a controller configured to control valves and pumps, which control and direct the flow of solutions and medium based on the steps of protocols, thereby automating the processes of decellularization, tissue sterilization, recellularization and self-sterilization. All parts of the device are installed in one single body in a fully integrated manner, in one example, which makes the device ready to use. The device minimizes the user intervention and enhances sterility, reproducibility, and efficiency.