An implantable hydrogel precursor composition can include: a cross-linkable polymer matrix that is biocompatible; and a plurality of polymer particles in the cross-linkable polymer matrix. The cross-linkable polymer matrix can include a cross-linkable hyaluronic acid polymer that has cross-linkable functional groups. The hyaluronic acid polymer can be a methacrylated hyaluronic acid polymer. The methacrylated hyaluronic acid polymer can have a molecular weight from about 500 kDa to about 1.8 MDa. The polymer particles can include a cross-linked hyaluronic acid. The cross-linkable polymer matrix having the polymer particles has a yield stress. The cross-linkable polymer matrix having the polymer particles has shape retention at physiological temperatures. The composition can include live cells in the cross-linkable polymer matrix. The composition can include a biologically active agent in the cross-linkable polymer matrix.

The invention provides methods and compositions for making and using collagen-glycosaminoglycan three-dimensional scaffolds immobilized with biomolecules that are spatially and temporally patterned. The method comprises adding benzophenone to a collagen-glycosaminoglycan three dimensional scaffold in the dark; adding one or more biomolecules to one or more areas of the collagen-glycosaminoglycan three-dimensional scaffold (which can be done optionally in the dark or in the light); and exposing the collagen-2glycosaminoglycan three-dimensional scaffold to light at a wavelength of about 350 to about 365 nm.

Graphene compositions used for neuronal repair and treatments, and, in particular neuronal scaffold-water soluble graphene for treatment of severed spinal cords and other neuronal repairs. The neuronal scaffold-water soluble graphene can be PEGylated GNR used in combination with a fusogen agent, such as PEG600.

The present invention relates to a peripheral nerve-specific hydrogel material, which is deliverable in a minimally invasive fashion, sustains the growth of neurons, and speeds recovery following surgical reconstruction.

The present disclosure relates to a silk fibroin tubular conduit, a new methodology for obtaining said silk fibroin tubular conduit, and respective uses. The tubular conduit of the present disclosure can be used in treatment of diseases that involve the repair and/or regeneration of tissues, nerves or bone. In particular, the tubular conduit can be used for peripheral nerve regeneration.

Provided are a hydrogel patch, a method of preparing the same, and a composition for the treatment of spinal cord injury including the hydrogel patch. The hydrogel patch enables a spinal cord injury patient to be treated in a manner which is non-invasive to the spinal cord.

The subject invention relates generally to the repair of severed or damaged nerve and/or epineurium in a patient in need of such treatment. In particular, the present invention provides devices and methods of repairing severed or damaged nerve and/or epincurium. In some embodiments, a device is provided for the in vivo repair of severed or damaged nerve and/or epineurium comprising: a pliable collagen membrane comprising a first surface and a second surface, said first surface having a plurality of protruding collagen bundles and said second surface comprising a plurality of apertures, said apertures and said collagen bundles arranged to form a self-engaging attachment when said first surface and said second surface are placed together.

A device, kit, and method for performing photochemical tissue bonding on a biological structure is provided. The device includes a first channel, a second channel, and an open window. The first channel is sized to receive a first portion of the biological structure, the second channel is sized to receive a second portion of the biological structure. The open window is positioned between the first channel and the second channel so that a third portion of the biological structure is exposed within the open window when the first channel receives the first portion and the second channel receives the second portion, where photochemical tissue bonding may be applied to the third portion.

Disclosed are systems, methods, and devices for tissue regenerative implants. In some aspects, a nerve tissue regeneration implant article includes an exterior shell; a plurality of fascicle structures disposed in an interior region of the exterior shell, where each fascicle structure includes a hollow region between a proximal end and a distal end, such that a fascicle structure is configured to facilitate and guide axonal growth along at least a portion of the fascicle structure between the proximal end and the distal end; and a plurality of vascularizable passages along the exterior shell, wherein the vascularizable passages are configured to allow vascular tissue to infiltrate the implant article, such that the implant article is able to facilitate nerve regeneration by enabling exchange of nutrients, oxygen, and/or waste to axons within the plurality of fascicle structures via the vascular tissue that infiltrates the implant article through the plurality of vascularizable passages.

Provided herein are populations of bone marrow mesenchymal stem cells (MSCs) and CD34+ hematopoietic stem/progenitor cells (HSPCs), and methods of isolation and co-administration thereof. In particular, cell populations and methods of co-administration thereof are provided for enhancing tissue (e.g., bladder) regeneration.