A method of preparing a decellularized placental membrane is provided. The method comprises removing cells from a pre-decellularized placental membrane comprising an amnion layer and a chorion layer to produce a decellularized placental membrane without separating the amnion layer from the chorion layer. The pre-decellularized placental membrane is obtained from an amniotic sac, and the decellularized placental membrane comprises the amnion layer and the chorion layer. Also provided is a decellularized placental membrane and a placenta-derived graft comprising the decellularized placental membrane. Further provided are the uses of the decellularized placental membrane or the placenta-derived graft.

A method of preparing a reconstitutable implantable bone putty includes combining a bone matrix derived from human bone and gelatin particulates derived from human tissue at a concentration of the bone matrix by dry weight of 20 to 60 percent to form the reconstitutable implantable bone putty. Preparing the gelatin particulates includes supplying a gelatin precursor of bone or soft tissue from a human, treating the gelatin precursor with phosphoric acid to generate a gelatin-acid mixture, neutralizing the gelatin-acid mixture with an alkali to a pH between 6 and 8 to allow a gelatin-rich solution and a waste solution to separate, removing residual salts from the gelatin-rich solution to obtain purified gelatin, drying the purified gelatin, and reducing the purified gelatin to particulates having a largest dimension less than 300 μm. A method of preparing an implantable bone putty includes adding a reconstitution media to the reconstitutable implantable bone putty.

The present invention relates to a dermal layer which is for grafting and has an improved graft survival rate, and a method for producing the same, wherein the dermal layer for grafting can be produced by filling a filling solution, including a DNA fragment mixture and chitosan, into an acellular dermal matrix from which cells have been removed. It was observed that the dermal layer for grafting produced in this manner, due to the filling solution filled therein and including a DNA fragment mixture and chitosan, increases the rate at which cells flow in from the tissue surrounding the graft and are fixed, and thereby alleviates an initial inflammatory reaction and promotes blending with the surrounding tissue.

A method for preparing a biocompatible tissue for revitalization thereof, wherein such tissue has an essentially tubular shape. The method provides inserting a support that mainly extends along a longitudinal extension axis inside the cavity defined by the tissue and performing on the outer and/or inner surface of the tissue a plurality of holes spread out on at least one of the generatrixes of the tissue. Holes are made with a depth which occupies at least a part of the thickness of the tissue.

The present invention provides an artificial bionic blood vessel and a manufacturing method thereof. The artificial bionic blood vessel includes a three-layer-structured artificial bionic blood vessel body, where the three-layer structure of the artificial bionic blood vessel consists of a natural silk layer, a diluted liquid silica-gel layer and a weaved tube layer, the diluted liquid silica-gel layer is located on the inner side of the natural silk layer, the weaved tube layer is located on the outer side of the natural silk layer, and the weaved tube layer is made of catgut by weaving.

An acellular soft tissue-derived matrix suitable for use as a medical graft or implant may comprise, for example, a delipidated, decellularized adipose tissue matrix. The delipidated, decellularized adipose tissue matrix is substantially free of substances that pose a significant risk of causing an immunogenic response in a patient receiving the matrix. Methods for producing the delipidated, decellularized adipose tissue matrix include the steps of delipidating an adipose tissue sample, followed by decellularizing the delipidated adipose tissue sample. The resulting delipidated, decellularized adipose tissue matrix contains a proportion of Type IV collagen which is greater than the proportion of Type IV collagen contained in a matrix produced by decellularizing an adipose tissue sample prior to delipidating. Additionally, the delipidated, decellularized adipose tissue matrix contains a proportion of lipids which is less than the proportion of lipids contained in a matrix produced by decellularizing an adipose tissue sample prior to delipidating.

The invention is directed to a composition comprising a fibrin hydrogel conjugated to peptides of laminin-111 (L1) and methods for repairing damaged salivary tissue using the composition.

This disclosure describes decellularized, biologically-engineered tubular grafts and methods of making and using such decellularized, biologically-engineered tubular grafts.

Provided herein is a bone repair composition that is composed of periosteum containing an angiogenic growth factor(s), cancellous bone chips containing viable osteogenic cells, and, optionally, demineralized bone matrix (DBM) chips. Also provided herein are articles of manufacture and methods of use thereof to treat bone defects.

The present invention provides a package comprising an aortoiliac artery graft and a record of a measured pressurized diameter of the aortoiliac artery graft, which measurement has been determined ex vivo under a pressure. A method of processing an aortoiliac artery graft is also provided. The processing method comprises subjecting an aortoiliac artery to a pressure ex vivo, and determining a measured pressurized diameter of the aortoiliac artery under the pressure. A method of treating abdominal aortic aneurysm, infected aortoiliac endograft or a traumatically damaged abdominal aorta or an iliac artery in a patient is further provided. The treatment method comprises anastomosing a processed aortoiliac artery graft with an aorta of the patient on the proximal end and the iliac or femoral arteries on the distal end, wherein a measured pressurized diameter of the processed aortoiliac artery graft has been determined ex vivo under a pressure.