Described herein are tissue grafts derived from the placenta with improved physical and biological properties. In one aspect, the tissue graft includes a first membrane comprising Wharton's jelly laminated with amnion, chorion, or a combination thereof. The presence of Wharton's jelly in the grafts enhances the performance of allograft amniotic-derived, caderivic allograft, xenograft, or alloplast soft tissue substitutes.

A tissue engineering material for nerve injury repair, a preparation method therefor and an application thereof. The tissue engineering material for nerve injury repair is an N-cadherin crosslinked linear ordered collagen scaffold. By crosslinking N-cadherin with a linear ordered collagen scaffold, the prepared tissue engineering material can efficiently induce migration of neural stem cells towards an injury region so that the neural stem cells are enriched in the injury region, and can effectively inhibit deposition of inhibitory factors such as chondroitin sulfate proteoglycan, promote differentiation of the neural stem cells into neurons, and then promote recovery of electrophysiological and motion functions. The N-cadherin crosslinked linear ordered collagen scaffold also has a stable ordered topological structure and excellent mechanical properties, and can be used to repair nerve injuries such as spinal cord injury.

Methods, compositions of matter, and devices for delivering a therapeutic composition to a heart of a subject using a biopolymer scaffold material are described. In some embodiments, the biopolymer scaffold material including the therapeutic composition may be attached to a first cardiac tissue of a subject. The therapeutic composition is delivered from the biopolymer scaffold material to the heart of the subject.

An injectable composite material for bone repair comprises a biological tissue material and bioceramics in order to serve as a three-dimensional scaffold for bone regeneration. The biological tissue material consists of microfibers having a naturally cross-linked structure without additional physical or chemical cross-linking, has superior biological compatibility, and can be slowly and completely degraded in vivo. The bioceramics in the composite material serves as a reinforcing phase. When combining the biological tissue material with the bioceramics, the composite material provides a template for bone tissue regeneration to effectively induce bone growth. The injectable composite material for bone repair can be used to fill bone defects, particularly critical-sized bone defects, and can be combined with a biological agent such as bone marrow to improve its biological activity. Therefore, the composite material can be widely used to repair bone defects caused by trauma, tumor resection, osteonecrosis, and infection.

The present invention relates to methods for removing antigens from tissues by sequentially destabilizing and/or depolymerizing cytoskeletal components and removing and/or reducing water-soluble antigens and lipid-soluble antigens. The invention further relates to tissue scaffolding and decellularized extracellular matrix produced by such methods.

This disclosure describes decellularized, biologically-engineered tubular grafts and methods of making and using such decellularized, biologically-engineered tubular grafts.

Compositions of small molecules, matrices, and isolated cells including methods of preparation, and methods for differentiation, trans-differentiation, and proliferation of animal cells into the osteoblast cell lineage were described. Examples of osteogenic materials that were administered to cells or co-cultured with cells are represented by compounds of Formula II, IV, and VI independently or preferably in combination with a matrix to afford bone cells. Small molecule-stimulated cells were also combined with a matrix, placed with a cellular adhesive or material carrier and implanted to a site in an animal for bone repair. Matrix pretreated with compounds of Formula II, IV, and VI were also used to cause cells to migrate to the matrix that is of use for therapeutic purposes.

A method is provided for producing a live cartilaginous material useful for implantation into a patient. A method of treating a patient comprising implanting a cartilaginous material prepared according to the provided method in an anatomical site in a patient also is provided.

The present disclosure relates to methods of preparing personalized blood vessels, useful for transplantation with improved host compatibility and reduced susceptibility to thrombosis. Also provided are personalized blood vessels produced by the methods and use thereof in surgery.

A tissue graft for soft tissue repair or reconstruction comprising a sheet of a biopolymer-based matrix having a plurality of small perforations and a plurality of large perforations. The small perforations are sized to facilitate clotting and granulation tissue development within the perforations which, in turn, facilitates revascularization and cell repopulation in the patient. The large perforations are sized to reduce the occurrence of clotting and granulation tissue development within the perforations so that extravascular tissue fluids accumulating at the implant site can drain through the tissue graft. The large perforations enhance mammal tissue anchoring by permitting mammal tissue to compress into the perforations increasing mammal tissue contact area.