In a method of manufacturing an object, a filament is fed to an extrusion head. The filament has a semi-crystalline polymeric reinforcement portion and a polymeric matrix portion. The temperature of the filament is raised in the extrusion head above the melting point of the matrix portion but below the melting point of the reinforcement portion so that the matrix portion of the filament melts within the extrusion head, thereby forming a partially molten filament within the extrusion head. The reinforcement portion of the partially molten filament remains in a semi-crystalline state as it is extruded from the extrusion head. Relative movement is generated between the extrusion head and the substrate as the partially molten filament is extruded onto the substrate in order to form an extruded line on the substrate. The matrix portion of the extruded line solidifies after the extruded line has been formed on the substrate.

A structure of aligned (e.g., radially and/or polygonally aligned) fibers, and systems and methods for producing and using the same. One or more structures provided may be created using an apparatus that includes one or more first electrodes that define an area and/or partially circumscribe an area. For example, a single first electrode may enclose the area, or a plurality of first electrode(s) may be positioned on at least a portion of the perimeter of the area. A second electrode is positioned within the area. Electrodes with rounded (e.g., convex) surfaces may be arranged in an array, and a fibrous structure created using such electrodes may include an array of wells at positions corresponding to the positions of the electrodes.

Bone tissue biomimetic materials, biomimetic constructs that can be formed with the materials, and methods for forming the materials and constructs are described. The bone tissue biomimetic materials include electrospun nanofibers formed of polymers that are conjugated with peptides that include acidic amino acid residues. The materials can incorporate high levels of mineralization so as to provide mechanical strength and promote osteogenesis and/or osteoconductivity on/in the bone tissue biomimetic materials. The materials and constructs can be utilized in forming tissue engineered structures for in vitro and in vivo use. Macroscopic bone tissue biomimetic scaffolds formed from the materials can be seeded with osteogenic cells and utilized to develop bone graft materials that can exhibit strength and osteoconductivity similar to the native bone and that exhibit uniform distribution of nutrients in the scaffolds.

According to one embodiment, a nozzle head includes a main body and a plurality of nozzles. The main body has a space in an interior of the main body. The space is capable of storing a source material liquid. The plurality of nozzles are conductive, are connected to the main body, and eject the source material liquid stored in the interior of the main body. An external dimension of one of the nozzles in a direction orthogonal to an extension direction of the nozzle is different from the external dimension of another one of the nozzles in the direction orthogonal to the extension direction of the nozzle.

Implementations described herein are directed to forming objects including one or more layers of a polymeric material that include a magnetic material. The objects can be produced by forming one or more first layers that include a first polymeric material. The one or more first layers can be free of a magnetic material. Additionally, the object can be produced by forming one or more second layers that include a second polymeric material having a magnetic material. For example, the one or more second layers can include a polymeric material embedded with magnetic particles. The one or more first layers and the one or more second layers can be formed by extruding the first polymeric material and the second polymeric material onto a substrate according to a pattern. A magnetizing device can be used to magnetize the magnetic material included in the one or more second layers.

A structure of aligned (e.g., radially and/or polygonally aligned) fibers, and systems and methods for producing and using the same. One or more structures provided may be created using an apparatus that includes one or more first electrodes that define an area and/or partially circumscribe an area. For example, a single first electrode may enclose the area, or a plurality of first electrode(s) may be positioned on at least a portion of the perimeter of the area. A second electrode is positioned within the area. Electrodes with rounded (e.g., convex) surfaces may be arranged in an array, and a fibrous structure created using such electrodes may include an array of wells at positions corresponding to the positions of the electrodes.

A film casting apparatus includes a die which has a non-zero angled discharge direction relative to a horizontal plane. A chill roll is positioned downstream of the die. A film displacement device is positioned proximate to the chill roll and is configured to position the molten polymer on the chill roll and establish the thickness of the film. The non-zero discharge angle is of a predetermined magnitude to gravity assist delivery of the molten polymer to the chill roll. The chill roll is positioned to gravity support the polymer film along a first length of a first side of the polymer film. A first polishing roll is positioned downstream of the chill roll and engages and cools a second length of a second side of the polymer film which is opposite the first side. The first length is substantially equal to the second length.

A multi-laminar electrospun nanofiber scaffold for use in repairing a defect in a tissue substrate is provided. The scaffold includes a first layer formed by a first plurality of electrospun polymeric fibers, and a second layer formed by a second plurality of electrospun polymeric fibers. The second layer is combined with the first layer. A first portion of the scaffold includes a higher density of fibers than a second portion of the scaffold, and the first portion has a higher tensile strength than the second portion. The scaffold is configured to degrade via hydrolysis after at least one of a predetermined time or an environmental condition. The scaffold is configured to be applied to the tissue substrate containing the defect, and is sufficiently flexible to facilitate application of the scaffold to uneven surfaces of the tissue substrate, and to enable movement of the scaffold by the tissue substrate.

A multi-laminar electrospun nanofiber scaffold for use in repairing a defect in a tissue substrate is provided. The scaffold includes a first layer formed by a first plurality of electrospun polymeric fibers, and a second layer formed by a second plurality of electrospun polymeric fibers. The second layer is combined with the first layer. A first portion of the scaffold includes a higher density of fibers than a second portion of the scaffold, and the first portion has a higher tensile strength than the second portion. The scaffold is configured to degrade via hydrolysis after at least one of a predetermined time or an environmental condition. The scaffold is configured to be applied to the tissue substrate containing the defect, and is sufficiently flexible to facilitate application of the scaffold to uneven surfaces of the tissue substrate, and to enable movement of the scaffold by the tissue substrate.

Compositions-of-matter comprising a matrix made of one or more, preferably two or more elastic layers and one or more viscoelastic layer are disclosed. The compositions-of-matter are characterized by high water-impermeability and optionally by self-recovery. Processes of preparing the compositions-of-matter and uses thereof as tissue substitutes or for repairing damaged tissues are also disclosed.