An embodiment of the invention is directed to a bone marrow aspiration device comprising a plurality of syringes, each of which operates in a series of sequential steps to obtain bone marrow of high quality and therapeutic value, i.e., having a high mesenchymal stem cell (MSC) MSC/ml number. In certain embodiments, a syringe may be used along with a collection vessel or with a second syringe. A further embodiment of the invention is directed to a method for using the bone marrow aspiration device as set forth herein.

The invention relates to an automatic device for the insertion of a stylet and cannula to a predefined depth within a bone, said depth being irrespective of the thickness of the tissue above the bone, which comprises: (a) a trigger unit for activating the device; (b) a barrel; (c) a probe unit within said barrel which is driven by a secondary spring, said probe unit comprises one or more probing needles at a distal end, and an anvil at a proximal end for defining an end of movement location for a hammer unit; (d) a hammer unit which is driven by a main spring, said hammer unit comprises a stylet at its distal end, a core at its proximal end, and a piston in between said core and stylet, wherein said stylet is inserted to within a cannula, and wherein when said hammer unit is positioned at said end of movement location, a tip of the stylet which is longer than said one or more probing needles is located a distance farther to the distal direction from the respective ends of said probing needles; and (e) one or more grasping units; wherein activation of said trigger unit follows by, (f) release of said second spring which in turn pushes said probing needles of said probe unit toward the patient's bone, up to a point of contact with the bone surface; (g) grasp of the anvil of said probe unit by said grasping units, when said contact of the probing needles with the patient's bone occurs; and (h) release of said main spring, which in turn pushes said hammer unit up to said end of movement location, thereby inserting said cannula to a depth of within the patient's bone.

Bone marrow harvesting devices and methods of use for harvesting bone marrow from a biological source of bone marrow are provided that promote ease of use, and reduce patient pain and potential trauma to harvested bone marrow, wherein an exemplary bone marrow harvesting device includes an elongated shaft comprising a distal region, a proximal region, and a lumen extending therebetween, the distal region comprising a plurality of through-wall apertures in fluid communication with the lumen; and an expandable member disposed along the distal region thereof and configured to transition between a collapsed state and an expanded state for vibrating to disrupt the bone marrow. Methods of using the inventive bone marrow harvesting device to provide more efficient bone marrow disruption, improve the efficiency of bone marrow aspiration, and increase the quantity of bone marrow harvested in a single procedure are also provided.

The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder (42), a pump (64) and a plurality of valves (1-11) configured to at least partially control fluid flow through a fluid circuitry and a separation column (40) positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column.

The embodiments disclosed herein generally relate to systems, devices and methods for the fractionation, isolation, extraction and processing of the adipose supernatant layer of a bone marrow aspirate. In particular, the various embodiments relate to systems devices and methods of obtaining, utilizing and processing the adipose supernatant layer of a bone marrow aspirate as a source of mesenchymal stem cells.

Compositions and methods for separating a sample of whole blood or bone marrow aspirate into a fraction rich in at least one of platelets and pluripotent cells are provided. A sample of whole blood can be centrifuged in a collection tube comprising a separator substance formulated to settle between the PRP fraction and the at least one other fraction. Preferably, centrifugation is completed without substantial activation of platelets. Optionally, the separator substance could be hardened to form a solid barrier that allows removal of all or substantially all of the platelets in the PRP fraction without remixing of the PRP fraction with the at least one other fraction. Transfer devices and methods are also provided in which a sterile sample can be transferred from a separation/preparation container (e.g., vacutainer) to a consumer or other container (e.g., dropper) while maintaining sterility of the sample.

The present invention provides devices and methods for concentrating a fluid and for treating a patient with the concentrated fluid. The concentrator apparatus includes a main housing (12) defining a separation chamber (14), a filter housing (48) containing a filter (46) comprising a filter element, a piping (44) for moving concentrated fluid from the separation chamber to the filter, and ports (32) for pressurizing the concentrated fluid past the filter element of the filter. The present invention also provides a variety of uses of concentrated body fluids, including autologous concentrated body fluid. The concentrated fluids can be used for example in surgical applications, including graft applications such as allograft, xenograft and autograft applications.

The present disclosure provides an inventive method of treating deteriorated skin tissue in the face or selected areas of the body, wherein the skin tissue has deteriorated due to aging and/or other factors, by injecting mesenchymal stem cells (MSCs) into these areas, resulting in improving and/or restoring the affected areas. In one aspect, the MSCs are obtained autogenously. In another aspect, the MSCs are minimally manipulated. In yet another aspect, treatment of the skin tissue is carried out using bone marrow concentrate that contains the MSCs. A platelet rich plasma (PRP) may be added to a bone marrow concentrate containing MSCs.

Methods and systems include an aspiration probe that includes a flutes section. The flutes section includes one or more longitudinal channels and another section that is coupled to the flutes section. The other section includes one or more openings corresponding to the one or more longitudinal channels. The flutes are configured to be placed within bone marrow through a hole in a bone. The openings are configured to be coupled to a suction device for generating suction. The flutes are configured to channel the bone marrow from within the bone into the openings based at least upon generating the suction.

A method for the long-term delivery of fluids to a bone of a patient includes providing a cannulated bone screw and an insert configured to be coupled to the bone screw. The method further includes creating an aperture in the skin of a patient, inserting the bone screw into a bone of the patient through the aperture, and coupling the insert to the bone screw. The method further includes the steps of providing a fluid source, coupling the fluid source to the insert, and delivering a fluid from the fluid source to the insert.