Bone marrow access apparatus includes a bone attachment part attachable to bone, a tubular conduit part attached to the bone attachment part and defining a conduit communicating with a hollow interior of the bone attachment part, and a port attached to the conduit part. The port defines a hollow interior communicating with the conduit defined by the conduit part. One or more optional flow control components are arranged in a passage defined by the interior of the bone attachment member, the conduit of the conduit part and the interior of the port and operatively restrict inflow or outflow. A localization part is on or integrated into the port and enables the port to be located through skin. In use, a sampling device is inserted through skin overlying the port, into and through the passage defined by the apparatus, through the flow control component(s), when present, and into the marrow space. Marrow is captured and then the sampling device is removed.

The present invention provides for the harvesting of specific materials in multiple stages of filtration of bone graft materials from a reaming device, specifics of interconnected stages, related filtration materials, and techniques. The harvesting process collects large material in a first stage, and other materials of a limited geometric size in at least a second stage of filtration. Such material captured in the second stage may contain plasma, cellular elements including stem cells as well as growth factors and other particulate matter of a specific geometrically limited size, using various filtration approaches including centrifugation in some embodiments. Further embodiments of the invention provide for an improved tubing interface and management approach to ease use in the operating room. Filtration materials may include biodegradable-material based filters and may allow direct implantation of small scale and larger scale matter in specific portions within the biodegradable-material itself.

A device for processing a biological material is disclosed. The device includes a syringe barrel comprising beads, a filter positioned at a close end of the barrel, a plunger insertable into the barrel through an open end, and a needle. The plunger includes a paddle assembly that is configured to mix a biological material with the beads after the biological material has been harvested from a patient.

The present disclosure relates to methods and apparatus for producing platelet rich plasma, bone marrow mononuclear cells, stromal vascular fraction from adipose tissue, and other concentrated or enriched biological fluids.

Provided herein is a triple syringe system that allows for a larger combined output of PRP (platelet rich plasma) and PPP (platelet poor plasma). The multi-syringe system allows for the connection of two or more additional syringes. The fractions may be extracted with the multi-syringe system of the present invention at different sequential times, or at the same time.

Methods of extracting bone and/or cellular material from a patient for possible use in a later surgical procedure, which involves that patient or even a different patient, are provided. An embodiment of the method generally comprises extracting bone and/or cellular material from a patient undergoing a routine surgical procedure, saving the material, and then sending it to a storage, separation, and processing facility (e.g., a biobank) for use in a later surgical procedure involving that patient, or a different patient. The material may be cancellous bone, cortical bone chips, or bone marrow. The extracted bone and/or cellular material can be processed at the biobank and used in a multitude of different medical procedures (e.g., as an allograft infused with stem cells for use in a fusion procedure).

The embodiments disclosed herein generally relate to systems, devices and methods for the fractionation, isolation, extraction and processing of the adipose supernatant layer of a bone marrow aspirate. In particular, the various embodiments relate to systems, devices, and methods of obtaining, utilizing, and processing the adipose supernatant layer of a bone marrow aspirate as a source of mesenchymal stem cells.

Tools, devices and systems that include a generally cylindrical, hollow shaft, and a suction canister are provided with advantageous interconnectivity features and functions that enable ease of manufacturing, lowered manufacturing costs, and allow for a platform of different shaft embodiments and canister embodiments that may be manufactured in different combinations to form an array of unique instruments. The interconnectivity may be irreversible or reversible, and the connection features maintain an air tight seal between the components. Tools, devices and systems are also provided that include collection functionality that can be employed to manipulate the volume of tissue collected while using a tissue collection feature/function. This may be undertaken to restrict total volume of the collection chamber in a way that allows for continuous suction flow rate to take place while collecting the desired amount of tissue. Containing the tissue to a pre-set volume can enable a user to effectively ascertain the volume of tissue collected.

The present invention provides for a removable second stage biocompatible substrate filter that includes biocompatible implant material configured to trap second stage operative particulate matter that may include at least one of bone fragments, plasma, stem cells, cellular matter, and growth factors captured from irrigation fluid. The second stage biocompatible substrate filter may be configured to combine with bone fragments captured from irrigation fluid by a first stage filter and may be configured to be operable with a reaming and collection device.

A body fluid component separating device according to an embodiment may comprise: a housing; a piston which divides the housing into a first space and a second space and connects the first space and the second space via fluid-connection; and a manipulator which is coupled to the piston to be operable and is configured to maintain a pressure balance between the first space and the second space, adjust movement of the piston between a first location at which the first space has a first volume and a second location at which the first space has a second volume that is different from the first volume, and fix the piston on an arbitrary location between the first location and the second location.