A body fluid component separating device according to an embodiment may comprise: a housing; a piston which divides the housing into a first space and a second space and connects the first space and the second space via fluid-connection; and a manipulator which is coupled to the piston to be operable and is configured to maintain a pressure balance between the first space and the second space, adjust movement of the piston between a first location at which the first space has a first volume and a second location at which the first space has a second volume that is different from the first volume, and fix the piston on an arbitrary location between the first location and the second location.

A method of delivering a therapeutic agent to a nucleus pulposus of an intervertebral disc is provided. The method comprises inserting a delivery tool containing the therapeutic agent through an anterior portion, a lateral portion, or an anterolateral portion of an annulus fibrosus and into the nucleus pulposus of the intervertebral disc and delivering the therapeutic agent to the nucleus pulposus of the intervertebral disc. Devices and kits are also provided.

A flexible cannula device includes a solid cannula shaft, a solid cannula tip including an opening, and a coil positioned between and welded to the cannula shaft and cannula tip, the cannula shaft, coil, and cannula tip forming a lumen in fluid communication with the opening. A flexible cannulated drill includes a solid cannula shaft, a solid cannula tip shaped to drill through tissue, a coil positioned between and welded to the cannula shaft and cannula tip, and a handle connected to the cannula shaft. The coil can be a double helix coil comprising an inner coil within an outer coil. A tube inside the coil prevents fluid from leaking between rings of the coil. A cannula system includes an introducer needle and the flexible cannula device. A screw mechanism can adjust a length that the flexible cannula device extends beyond a distal end of the introducer needle.

The present invention is related to the field of tissue regeneration. It concerns more particularly new standardizations and medical devices for the preparation of A-PRP, PRP, BMC, fat tissue, alone or in combination with a biomaterial or cell extract.

A spinal drainage kit container includes a body, lid, and hinge; the body includes a peripheral portion and a first grip that is gripped when the lid is to be opened; the first grip is at a first corner of the body, opposite the hinge; the first grip has a first protrusion protruding in a first direction; the lid includes a contact that contacts the peripheral portion when the lid is closed, and a second grip that is connected to the contact and is gripped when the lid is to be opened; the second grip is at a second corner of the lid, which overlaps with the first corner when the lid is closed; and the second grip has a second protrusion that protrudes in a second direction and does not overlap with the body when the lid is closed, and the second direction is orthogonal to the first direction.

Compositions and methods for separating a sample of whole blood or bone marrow aspirate into a fraction rich in at least one of platelets and pluripotent cells are provided. A sample of whole blood can be centrifuged in a collection tube comprising a separator substance formulated to settle between the PRP fraction and the at least one other fraction. Preferably, centrifugation is completed without substantial activation of platelets. Optionally, the separator substance could be hardened to form a solid barrier that allows removal of all or substantially all of the platelets in the PRP fraction without remixing of the PRP fraction with the at least one other fraction. Transfer devices and methods are also provided in which a sterile sample can be transferred from a separation/preparation container (e.g., vacutainer) to a consumer or other container (e.g., dropper) while maintaining sterility of the sample.

Bone marrow containers are described herein. Separation systems for separating activated immune cells from other components are described herein. Methods of activating an immune cell are described herein.

A coiled shaft for tissue disruption is described herein where a flexible aspiration cannula has a first portion and a second portion formed of the coiled shaft. The cannula is configured in a particular embodiment to rotate over the length of the cannula to disrupt the matrix of bone marrow without the cannula buckling or collapsing. The cannula also includes a disruption tip coupled to the coiled shaft. The disruption tip has a radiused portion along a distal tip face of the disruption tip.

The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder (42), a pump (64) and a plurality of valves (1-11) configured to at least partially control fluid flow through a fluid circuitry and a separation column (40) positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column.

The present invention provide a puncture instrument capable of administering (supplying) a drug solution. The puncture instrument includes a puncture tip section (2); a first tubular body (3) connected to the puncture tip section (2) at the distal end; and an outer tubular body (5) at least partially covering the first tubular body (3). The first tubular body (3) is formed to be rotatable around an axis along the longitudinal direction. The first tubular body (3) has an outer diameter smaller than an inner diameter of the outer tubular body (5). A drug solution supply path (5a) is provided on the outside of the first tubular body (3).