The invention discloses a medium combination for a rapid propagation medium for blueberry stem segment tissue, wherein the pre-culture medium takes the MS medium containing 2-(N-morpholine) ethanesulfonic acid as basic medium, comprising 0.1-0.5 mg/L IAA, 0.05-0.6 mg/L CPPU; the rapid culture medium takes the MS medium containing 2-(N-morpholine) ethanesulfonic acid as basic medium, comprising: 0.2-0.5 mg/L ZT; the seedling growth medium takes the MS medium containing 2-(N-morpholine) ethanesulfonic acid as basic medium, comprising: 0.1-2.0 mg/L 2-ip; and the rooting medium takes MS medium as basis medium, comprising: 0.3-4.0 mg/L NAA. The invention further discloses a method of using the above medium combination to conduct rapid propagation of blueberry stem segment tissue, the propagation rate of this method is rapid.

A method of cultivating plant in transparent sealed container and base used therefor, wherein the base is mainly obtained by solidifying a mixture formed by adding gellan gum to transparent nutritional liquid; the gellan gum being added to the nutritional liquid is in an amount of 2-10 g/L; the base has a PH value of 3.5-10.0. The use of gellan gum as the sole transparent solidifying agent ensures the gellan gum, the base of the present invention is capable of adhering securely in the transparent sealed container. As shown by experiments, the base of the present invention will not slide or disperse in the transparent sealed container under normal shaking state, and can remain adhered to the transparent sealed container for 3-10 minutes when the transparent sealed container is turned upside down.

Containers for growth of sprouts are described. The covered containers have an air-permeable seal and may also have additional openings to increase the air permeability rate of the covered container. The air permeability rate is balanced to have sufficient air exchange but with an evaporation rate that is low enough to avoid addition of water during germination and growth of the cryo-sprouts. The seeds and sufficient water are added to the container and covered with a lid to form an air-permeable covered container. The seeds germinate and grow in the covered container at pathogen antagonistic temperature without the removal of the lid until use by consumer. This results in cryo-spouts in that have reduced numbers of pathogenic organisms, are greener and have an extended shelf-life.

A method, apparatus, and system are provided for the printing and maturation of living tissue in an Earth-referenced reduced gravity environment such as that found on a spacecraft or on other celestial bodies. The printing may be three-dimensional structures. The printed structures may be manufactured from low viscosity biomaterials.

A temporary immersion bioreactor (10) for in vitro production of differentiated plant biomass including a growth chamber (12) having one or more transparent side walls (14) and a mesh bottom (16), the mesh bottom (16) defining a plurality of pores (18) to receive plant material. The bioreactor (10) includes a flexible bag (20) formed from a transparent material, the flexible bag (20) having a sealable opening and being dimensioned to receive the growth chamber (12) together with a liquid medium (22). The bioreactor (10) also includes an outer chamber (24) having one or more transparent side walls (26). the outer chamber (24) is formed to correspond in shape to the growth chamber (12) and dimensioned to receive the growth chamber (12) within the flexible bag (20) so that the mesh bottom (16) of the growth chamber (12) faces a bottom (28) of the outer chamber (24) that in use is intended to rest on a support surface. Movement of the growth chamber (12) within the outer chamber (24) is restrained to movement along a single axis such that the mesh bottom (16) of the growth chamber (12) moves towards and away from the bottom (28) of the outer chamber (24).

The present invention discloses and claims methods and devices for the rapid mechanical isolation of monocot plant tissues suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating target plant tissues for use as transformable explants, and propagation of transgenic plants and plant tissues.

A container for effecting at least one non-lethal change in inherent properties of a non-human biological system, such as a plant or a fungus, under the influence of zero gravity, comprising a carrier plate configured to receive two capacitor plates of a plate capacitor, wherein the plates are situated parallel to one another and in each case perpendicularly on the carrier plate, at least one sample container arranged horizontally on the carrier plate, adjacent to each of the two capacitor plates and configured to receive the biological system, and an electromagnet arranged substantially half way between the capacitor plates and, when seen from the carrier plate, arranged horizontally above the at least one sample container. Additionally, a European modular cultivation system for use in the International Space Station comprises a centrifuge, wherein the container is received in the centrifuge.

Methods and systems are provided for singulating, orienting, and delivering oriented objects (e.g., embryos) to a desired location (e.g., a plate containing a selected medium).

Methods and systems are provided for singulating, orienting, and delivering oriented objects (e.g., embryos) to a desired location (e.g., a plate containing a selected medium).

Systems, methods, and apparatus for large scale, high throughput phenotyping of items including plants to derive environment/genetics correlations based on controlled variation of environment. Spatial and temporal resolution is improved by an integrated system that can use space efficiently yet concurrently process a large number of replicates but in varying environments. This is accomplished by using an array of miniature greenhouses, each in communication with one or more generators or regulators of an environmental factor that can be independently supplied and controlled to each miniature greenhouse. The controller also controls acquisition of data from each greenhouse as well as processing of that data into phenomic/genomic correlations.