The present invention relates to a method for Agrobacterium-mediated transformation and regeneration of Stevia plants. In particular, the method involves co-culturing leaf explants with Agrobacterium in a medium comprising acetosyringone and 2,4-dichlorophenoxyacetic acid in the dark, callus induction and shoot regeneration in a medium comprising 6-benzylaminopurine, 3-indoleacetic acid, a selective agent and an Agrobacterium eradicant in the dark, and root regeneration in a medium comprising 3-in-doleacetic acid in a light/dark cycle. The present invention also relates to the overexpression of SrDXS I and SrKAH in transgenic plants, resulting in the enhancement of steviol glycosides in the transgenic plants. The present invention further relates to the overexpression SrUGT76G I in transgenic plants, resulting in higher Rebaudioside A (Reb A) to stevioside ratios in the transgenic plants.

A plant propagation system is provided that includes a holder for holding at least two plants in relative spaced apart relation to enable a predetermined operation (such as, for example, a cutting operation) to be performed on each of the plants within the holder during a single pass.

The present disclosure provides an insulation layer composition and an insulation layer for a plant tissue culture. The insulation layer composition includes polyvinyl acetate emulsion resin, water, a gel forming agent and a hyaluronic acid diluent, in which the gel forming agent is a mixture of glycerin and polyglycerol acrylate. The insulation layer for the plant tissue culture includes the insulation layer composition.

The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.

The present invention provides a biodegradable substrate for supporting growth of seeds or clones. The substrate comprises: about 70% to about 95% hemp fibre and about 5% to about 30% of a biodegradable thermoplastic polymer. The present invention also provides a process for preparing a biodegradable substrate for supporting growth of seeds or clones from a hemp fibre-based mat.

A method, apparatus, and system are provided for the printing and maturation of living tissue in an Earth-referenced reduced gravity environment such as that found on a spacecraft or on other celestial bodies. The printing may be three-dimensional structures. The printed structures may be manufactured from low viscosity biomaterials.

The present invention relates to a method for Agrobacterium-mediated transformation and regeneration of Stevia plants. In particular, the method involves co-culturing leaf explants with Agrobacterium in a medium comprising acetosyringone and 2,4-dichlorophenoxyacetic acid in the dark, callus induction and shoot regeneration in a medium comprising 6-benzylaminopurine, 3-indoleacetic acid, a selective agent and an Agrobacterium eradicant in the dark, and root regeneration in a medium comprising 3-indoleacetic acid in a light/dark cycle. The present invention also relates to the overexpression of SrDXS1 and SrKAH in transgenic plants, resulting in the enhancement of steviol glycosides in the transgenic plants. The present invention further relates to the overexpression SrUGT76G1 in transgenic plants, resulting in higher Rebaudioside A (Reb A) to stevioside ratios in the transgenic plants.

A tissue culture method and a propagation method of Cathaya argyrophylla are described herein. An adventitious bud can be induced from an explant of the Cathaya argyrophylla using a suitable induction medium, with an induction rate reaching 69.22% to 73.33%. The induced adventitious bud is inoculated into a suitable proliferation medium, such that the adventitious bud can proliferate and strengthen seedlings, where the adventitious bud shows a proliferation rate reaching 46.23% to 55%. The adventitious bud after proliferation and seedling strengthening are inoculated into a suitable rooting medium to allow rooting culture, thereby obtaining a complete tissue culture seedling of the Cathaya argyrophylla. The tissue culture method is of great significance for protecting the Cathaya argyrophylla, increasing a plant quantity of the Cathaya argyrophylla, and expanding a population of the Cathaya argyrophylla to prevent extinction.

A method, apparatus, and system are provided for the printing and maturation of living tissue in an Earth-referenced reduced gravity environment such as that found on a spacecraft or on other celestial bodies. The printing may be three-dimensional structures. The printed structures may be manufactured from low viscosity biomaterials.

A nutrient medium for the cultivation of plants, containing (a) 3 g/l to 18 g/l agar and (b) 0.5 g/l to 3 g/l carrageenan, as well as plant plugs which contain such a nutrient medium, a method for the production of the plant plugs, and a method for the automated or semi-automated cultivation of plants using the plant plugs.