The present disclosure provides compositions and methods for producing a rose plant using somatic embryogenesis. In particular, the present disclosure provides novel methods and media for rose callus induction, embryoid suspension, and embryo regeneration. Unlike currently available techniques, the novel compositions and methods provided herein produce high yields of competent cells and high frequencies of rose somatic embryogenesis.

A method of producing cotton fibers from cotton callus cells in vitro is provided. The method includes the following steps in order: (1) culturing cotton callus cells in a suspension culture medium; (2) feeding a first bioreactor with the callus cells, a multiplication medium, and air compression; (3) culturing the callus cells in the first bioreactor for 5 to 12 days to accomplish at least two rounds of duplication of the callus cells; (4) serially repeating steps (2) and (3) one or more times, thereby obtaining serially duplicated cells; (5) seeding one or more second bioreactors with the serially duplicated cells, an elongation medium, and air compression; (6) culturing the serially duplicated cells in the one or more second bioreactors, thereby obtaining elongated cells; and (7) culturing the elongated cells in a maturation medium, thereby obtaining the cotton fibers.

Provided is a method for separating and culturing ginseng stem cells, including the steps of: (1) culturing adventitious roots of ginseng by a one-step method; (2) taking root tips of the adventitious roots of ginseng, and dissecting and separating to obtain a stem cell area; (3) isolation and culture: inoculating the stem cell area into a stem cell induction medium for dark culture to obtain stem cell masses; (4) subculture: picking part of the stem cell masses obtained in (3) to be inoculated into a stem cell subculture medium for dark culture; and (5) liquid culture: inoculating stem cells obtained in (4) into a stem cell liquid medium for dark culture to obtain ginseng stem cells.

Embodiments described herein provide for multi-media structures 100 with growth enhancement additives for multiple stages of growth of an organism such as a plant, fungus or bacteria, including the production of individual media structures and multi-media structures 100 for multi-stage growth. Methods for the production of individual media structure and multi-media structures 100 with growth enhancement additives are provided. Methods for using multi-media structures 100 to grow an organism through multiple stages of growth such as root production, vegetative growth and flowering are also provided.

The present invention relates to a method for the production of haploid, polyhaploid and/or doubled haploid embryos, calli, seeds and/or plants of the species Helianthus annuus from isolated microspore cultures, more specifically to a method comprising contacting the isolated microspores with a histone deacetylase inhibitor (HDACi) and a complex protein composition. The present invention also provides a kit for producing a haploid, polyhaploid and/or doubled haploid embryo, callus, seed and/or plant of the species Helianthus annuus from at least one isolated microspore as well as the use of a histone deacetylase inhibitor (HDACi) and a complex protein composition for producing a haploid, polyhaploid and/or doubled haploid embryo, callus, seed and/or plant of the species Helianthus annuus. Finally, the present invention also relates to a population of haploid, polyhaploid and/or doubled haploid Helianthus annuus plants directly derived from at least one sunflower apical or lateral capitula.

Described herein are methods of producing an organogenic callus along with compositions, systems, and methods relating thereto.

It comprises a receptacle for the culture of plant material, a cap for closing an opening to said receptacle, and means for allowing the entry and/or exit of gas from said receptacle, and is characterized in that it comprises a container for receiving a liquid culture medium inside the receptacle, and support means for supporting the plant material on said reception container, wherein said container is attached to the lid of the receptacle in a position suitable for use, said lid including at least one medium inlet and/or exit conduit that connects with the container to be able to fill and/or empty said container through said conduit.

Provided are methods and systems for propagating plant material and the plant cells obtained thereby. The method comprises providing a solution comprising a plurality of plant protoplasts comprising an encapsulation medium or encapsulation medium precursor, introducing the solution into a microfluidic device, forming droplets of the solution in the microfluidic device, at least some of which encapsulate a single protoplast, causing the encapsulation medium or encapsulation medium precursor to gelify in the microfluidic device, and collecting the encapsulated protoplasts. The system comprises a microfluidic device comprising a droplet generator, a first injection system comprising a first liquid and configured for injecting the first liquid into the droplet generator, and a second injection system comprising a second liquid and configured for separately injecting a second liquid that is immiscible with the first liquid into the droplet generator. The first liquid is a solution comprising protoplasts and the first liquid comprises an encapsulation medium or encapsulation medium precursor, and the system is configured such that droplets are formed in the droplet generator, each droplet enclosing a single or no protoplast and comprising the encapsulation medium or encapsulation medium precursor. The encapsulation medium or encapsulation medium precursor in the droplets is gelified in the microfluidic to generate gelified droplets enclosing a single or no protoplast.

The invention pertains to a method for producing a plant shoot, wherein said method comprises the step of locally contacting a plant tissue comprising a sequence encoding at least one regeneration factor under the control of an inducible promoter with a inductive hydrogel. The invention further pertains to an inductive hydrogel and the use of said inductive hydrogel for inducing regeneration.

A technology regarding the production, formulation and use of conditioned media and the factors included therein is disclosed. The conditioned media may be inoculated with animal cells, plant cells and any combination thereof. The inoculations may occur simultaneous or at different times. Cells retrieved from different areas of the animal and/or the plant may also be cultured together to form conditioned media and associated growth factors.