Disclosed herein are methods for the production of Cannabis meristem explants from dry seeds. Also described are methods of transforming and gene editing using the Cannabis meristem explants disclosed herein.

A vertically-integrated Cannabis-related product production method is described, the method comprises, producing a distilled cannabinoid and/or a crystallized cannabinoid from Cannabis plants, comprising: in a farming system, growing the Cannabis plants, the Cannabis plants comprise a cannabinoid; in an extraction system, extracting the cannabinoid from the Cannabis plants; in a purification system, purifying the cannabinoid to produce a purified cannabinoid; and in a distillation and/or a crystallization system, distilling and/or crystallizing the purified cannabinoid to produce the distilled cannabinoid and/or the crystallized cannabinoid. Various ways to purify, distill, and process the cannabinoids are described. An insect pest management system may be integrated with the farming system to grow the Cannabis plants in the presence of predatory mites which feed on insects and/or spider mites.

The present invention relates to a Solanum lycopersicum seed designated 72-013 RZ. The present invention also relates to a Solanum lycopersicum plant produced by growing the 72-013 RZ seed. The invention further relates to methods for producing the tomato cultivar, represented by tomato variety 72-013 RZ.

Numerous plant microbes, including the vascular-limited Candidatus spp.—causal agents of citrus greening and potato zebra chip diseases—are non-culturable. The present disclosure relates, according to some embodiments, to compositions, methods and systems for culturing such organisms. For example, the present disclosure relates to methods for culturing, propagating, and characterizing fastidious vascular-colonizing microbes using a hairy root system (e.g., in vitro, in planta). The present disclosure relates, in some embodiments, to methods for cultivating a fastidious plant microbe including: contacting a plant (e.g., a tomato plant, a potato plant, a citrus plant) colonized by a fastidious plant microbe (e.g., Xylella fastidiosa, Candidatus Liberibacter spp.) with a suspension of R. rhizogenes under conditions that permit induction of hairy roots colonized with the fastidious plant microbe, and propagating the colonized microbial hairy roots.

Methods of propagating a Cannabis cutting are provided. A method of propagating a Cannabis cutting can include: providing a coherent growth substrate comprising man-made vitreous fibres bonded with a cured binder composition, the growth substrate having a density in the range of 60 kilograms per cubic meter (kg/m.sup.3) to 70 kg/m.sup.3; inserting the Cannabis cutting into the growth substrate at a location where the growth substrate does not have a seed hole; and providing a nutrient solution having an electrical conductivity value between 1.6 milli-Siemens per centimeter (mS/cm) and 2.4 mS/cm to the Cannabis cutting in the growth substrate.

A new and distinct horseradish (Armoracia rusticana) named K-62 is disclosed, characterized by and distinguished from prior commercial horseradish varieties by increased activity of horseradish peroxidase (HRP). Plants have been clonally propagated by root division and shown to retain the increased HRP activity, which can be used, among other things, in molecular biology applications.

Methods of propagating a Cannabis cutting are provided. A method of propagating a Cannabis cutting can include: providing a coherent growth substrate comprising man-made vitreous fibres bonded with a cured binder composition, the growth substrate having a density in the range of 60 kilograms per cubic meter (kg/m.sup.3) to 70 kg/m.sup.3; inserting the Cannabis cutting into the growth substrate at a location where the growth substrate does not have a seed hole; and providing a nutrient solution having an electrical conductivity value between 1.6 milli-Siemens per centimeter (mS/cm) and 2.4 mS/cm to the Cannabis cutting in the growth substrate.

The present invention relates to a method for producing haploid, dihaploid, polyhaploid and/or doubled haploid plants of the family Cucurbitaceae from isolated microspores, wherein said method comprises a) culturing isolated microspores to obtain embryos competent for plant regeneration, wherein the microspores have been isolated from plant material of a donor plant of the family Cucurbitaceae; and b) regenerating plants from the embryos; wherein step (a) comprises contacting the microspores with one or more inhibitor of histone deacetylase (HDACi) and one or more polyamine. The present invention also relates to a method for producing haploid, dihaploid, polyhaploid and/or doubled haploid embryos, to related kits and compositions, and to plants obtained according to the methods.

A method for culturing ginseng cell with high content of ginsenoside, including inducing ginseng cell line: after disinfected and sliced, ultrasonically treating old mountain ginseng, and culturing the old mountain ginseng in a culture medium; screening the ginseng cell line: choosing a variety of culture mediums and using hormones for cell separation and culture, selecting cell lines with better growth morphology and faster growth, and performing solid subculture and liquid suspension culture; optimizing conversion conditions: using acids to treat the chosen cell lines, and controlling the transformation temperature and transformation time, detecting ginsenosides Rg3 and Rh2 in the dried products, determining an optimal transformation condition according to the highest total amount; large-scale industrial production: according to the optimal transformation condition, performing the liquid suspension culture of the selected cell lines and scaling up the scale of culture to obtain large-scale industrial production of ginseng cell products.

Described is a plant transformed with one or more genes originating from the Ri plasmid of Agrobacterium rhizogenes by infection with A. rhizogenes comprising the Ri plasmid, or being progeny of such a plant, said plant or progeny comprising, in the genome thereof, 1 to 5 copies said one or more genes originating from the Ri plasmid. Further, the use of such a plant or progeny thereof as ornamental plants, the use as field crop species, for food extracts, cosmetics, perfumes or as medicinal plants is disclosed. Further disclosed are methods for the preparation of such plants. Said plants display an intermediate height and/or with a higher content of metabolites without significant reduction of flower number or flowering time delay as compared to control plants void of Agrobacterium rhizogenes sequences.