A method of producing cannabinoids for use in medical treatments by growing cultured Cannabis sativa plant cells through tissue culture, the method comprising the steps of: selecting Cannabis sativa leaf tissue for culture; and growing a tissue culture from the selected leaf tissue in a liquid based medium whilst controlling the light exposure of the tissue culture to control the cannabinoid content of the tissue culture. Control of the light exposure can enable the phytocannabinoid content of the grown tissue culture to be tailored to the use intended for the tissue culture. For example, the THC content of the tissue culture can be controlled to be maximised or minimised depending on the intended use. Use of tissue culture is beneficial as compared to prior art methods as it allows for genetic consistency and reduces the resources necessary to produce plant cells containing phytocannabinoids.

Provided herein are methods for establishing Theobroma cell suspension cultures using young leaves as a source of explants. Also provided are induction, proliferation, and suspension media used for producing Theobroma cell suspension cultures. These methods and media may be useful for producing secondary metabolites in Theobroma, as well as for isolating virus particles associated with Theobroma diseases.

Sunflower is an important oilseed crop. Typically, new cultivars with improved agronomic traits are developed through conventional breeding program. Here, we describe a method of sunflower embryo rescue to accelerate trait introduction into plants. Further, weeds are a significant issue for the sunflower crop. However, there are not many selective herbicides for sunflower. Here, using the embryo rescue methodology, we further describe in vitro herbicidal section methodologies.

The invention provides an improved method for transformation of sorghum and the production of genetically modified sorghum. In particular, methods and means are described for the production of high quality, transformable sorghum plant cells which are maintained in culture for longer duration without losing their regenerative potential and their use for genetic transformation. The method also describes the use of improved media for efficient plant regeneration from transformed plant cells, thereby providing significant improvement in the stable transformation frequency of sorghum.

The invention relates to the grafting of perennial monocots such as bananas and oil palms. Processes for the production of such plants are disclosed herein.

A method to expedite the growth of the agave plant by growing the plant tissue from a cell culture in a laboratory utilize incubator cells that are taken from the leaf of an agave plant and grown in a medium containing all the nutrients required for the production of new cells and the maturation of those new cells. The cells are then exposed to photosynthetic active radiation and fed with extra carbon dioxide, causing the cells to undergo photosynthesis and form the sugars that are found in a naturally growing agave plant. These cultivated cells are given an optimum amount of light and carbon dioxide to promote an unexpected and substantially higher growth rate than heretofore achieved.

A Stevia extract made from leaves of the Stevia rebaudiana plant is described. The extract has desired levels of steviol glycosides and is useful in food, beverage, and other consumable products.

A novel hybrid sweet corn plant, designated COACHMAN is disclosed. The disclosure relates to the seeds of hybrid sweet corn designated COACHMAN, to the plants and plant parts of hybrid sweet corn designated COACHMAN, and to methods for producing a sweet corn plant by crossing the hybrid sweet corn COACHMAN with itself or another sweet corn plant.

An inbred corn line, designated 7WWXL2020, the plants and seeds of the inbred corn line 7WWXL2020, methods for producing a corn plant, either inbred or hybrid, produced by crossing the inbred corn line 7WWXL2020 with itself or with another corn plant, and hybrid corn seeds and plants produced by crossing the inbred line 7WWXL2020 with another corn line or plant and to methods for producing a corn plant containing in its genetic material one or more transgenes and to the transgenic corn plants produced by that method. This invention also relates to inbred corn lines derived from inbred corn line 7WWXL2020, to methods for producing other inbred corn lines derived from inbred corn line 7WWXL2020 and to the inbred corn lines derived by the use of those methods.

An inbred corn line, designated 3KKXX0202, the plants and seeds of the inbred corn line 3KKXX0202, methods for producing a corn plant, either inbred or hybrid, produced by crossing the inbred corn line 3KKXX0202 with itself or with another corn plant, and hybrid corn seeds and plants produced by crossing the inbred line 3KKXX0202 with another corn line or plant and to methods for producing a corn plant containing in its genetic material one or more transgenes and to the transgenic corn plants produced by that method. This invention also relates to inbred corn lines derived from inbred corn line 3KKXX0202, to methods for producing other inbred corn lines derived from inbred corn line 3KKXX0202 and to the inbred corn lines derived by the use of those methods.