This document relates to methods and materials for genome engineering in eukaryotic cells, and particularly to methods for increasing genome engineering (i.e. transformation or genome editing) efficiency via delivery of one or more RKD2 and RKD4 genes, with genome engineering components.

A plant medium composition and methodology of application of the medium as it pertains to the micropropagation of Cannabis and related plant species is described that yields two to four-fold increase in the number of biologically functional nodes from axillary shoots thereby allowing for exponential amplification of such plant species. The compositions comprise basal plant media supplemented with: (i) one or more cytokinins, and (ii) one or more gibberellins and/or brassinolides. This achievement in exponential shoot growth of these plant species represents a marked step forward in the Cannabis industry as it pertains to commercial micropropagation of a recreationally and medicinally important plant whose extracts are the subject of numerous clinical trials.

The present invention provides a longitudinally split immature tiller separated from a rhizome (LSITR), a duster of multiple in vitro shoots (CMIT), a cluster of stem segments containing shoot primordia (CSSSP) and an in vitro tiller of Miscanthus x giganteus (Giant miscanthus), and their uses in propagating Miscanthus x giganteus (Giant miscanthus).

A method for producing haploid Lolium plants may start with providing a Lolium multiflorum inducer line, the L. multiflorum inducer line having the ability to induce mitotic genome instability and haploid sectoring when hybridized as a maternal parent with a Lolium sp. paternal parent, such as previously disclosed lines IL1 and IL2. The inducer line may then be crossed with a Lolium sp. to generate F1 plants, and the F1 plants may be self-fertilized so as to recover seed from the selfed plant. The recovered seed may then be planted to generate one or more F2 plants, and at least one of the F2 plants may be a haploid Lolium plant.

The present invention relates to a watermelon plant showing resistance against potyviruses, wherein the watermelon plant may comprise one or more of resistance conferring alleles of QTL1, QTL2 and QTL3 and wherein the said QTLs are as present in or obtainable from a watermelon plant, representative seed of which was deposited under deposit accession number NCIMB 42537, 42536, 42535 respectively.

The present disclosure provides approaches for reducing tobacco-specific nitrosamines (TSNAs) in tobacco. Some of these approaches include genetically engineering tobacco plants to increase one or more antioxidants, increase oxygen radicle absorbance capacity (ORAC), or reduce nitrite. Also provided are methods and compositions for producing modified tobacco plants and tobacco products therefrom comprising reduced TSNAs.

Provided are a method for improving rice yield by jointly knocking out ABA receptor PYL family genes and a use thereof.

This document relates to methods and materials for genome engineering in eukaryotic cells, and particularly to methods for increasing genome engineering (i.e. transformation or genome editing) efficiency via co-delivery of combinations of one or more booster polypeptides, and boost genes, with genome engineering components.

Materials and methods for inducing genetic alterations in meristematic plant tissue are provided herein.

The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.