An in vitro rescue method for immature embryo of avocado includes: collecting immature avocado hybrid fruits, stripping immature zygotic embryos from the sterilized fruits; inducing maturation of zygotic embryos in different media selected according to the maturities of zygotic embryos; and avocado seed embryos growing to normal plants under light conditions after germination. Provided is a technology for the in vitro culture rescue of immature embryo of avocado, wherein the zygotic embryos at different developmental stages are induced to maturation under in vitro conditions, and after maturation, the seed embryos are induced to germinate and form healthy plants. The present invention can effectively improve the survival rate of hybrid avocado zygotic embryo, increase the success rate of hybrid avocado, rescue the hybrid offspring, reduce the loss of hybrid offspring, ensure the genetic integrity of hybrid group, and provide the key technology for hybrid breeding and genetic research of avocado.

A Stevia extract made from leaves of the Stevia rebaudiana plant is described. The extract has desired levels of steviol glycosides and is useful in food, beverage, and other consumable products.

The present invention relates to excision of explant material comprising meristematic tissue from cotton seeds. Methods for tissue preparation, storage, transformation, and selection or identification of transformed plants are disclosed, as are transformable meristem tissues and plants produced by such methods, and apparati for tissue preparation.

The present disclosure includes the production of a mutant plant resistant to an herbicide of the phosphonomethylglycine family, e.g. glyphosate. Compositions and methods are provided for editing a nucleotide sequence of interest in a cell employing a guide polynucleotide/Cas endonuclease system, wherein the Cas endonuclease is guided by a guide polynucleotide to recognize and optionally introduce a double strand break at a specific target site into the genome of a cell. The nucleotide sequence of interest to be edited can be located within or outside the target site that is recognized by a Cas endonuclease. More specifically, compositions and methods are provided for editing an enolpyruvylshikimate-3-phosphate synthase (EPSPS) nucleotide sequence in a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide for an effective system for editing EPSPS nucleotide sequences of within the genome of a cell. Also provided are compositions and methods for the production of glyphosate tolerant plant cells, plants explants, seeds and grain.

There are provided compositions and methods for transforming plants, preferably monocot, and even more preferably, sugarcane. The transformation methods involve infection of plant tissue with Agrobacterium, and co-cultivation using culture medium comprising high concentrations of gelling agent, with the result of inhibiting the exacerbated growth of the bacteria and increasing the transformation frequencies. The invention includes regenerating transformed plants, and the transformed plants themselves.

A hybrid mint plant characterized by an essential oil composition profile, methods of cultivating the hybrid mint plant, and methods of producing an essential oil composition with the essential oil composition profile using the hybrid mint plant are disclosed.

The present application relates to methods for the production of plant material suitable for the extraction and purification of saponins, through plantations of clone plants of defined chemotype, including ultrahigh density plantations of Quillaja saponaria Molina, and methods to increase the recovery of saponins by solid/liquid extraction of the harvested plant material.

The invention relates to a dominant mutation in the TDM gene leading to the production of 2n gametes in plants, to the plants comprising said mutation, and to their use in plant breeding. The invention relates also to plants in which the dominant mutation in the TDM gene is combined with the inactivation of a gene involved in meiotic recombination in plants and a gene involved in the monopolar orientation of the kinetochores during meiosis. These plants which produce apomeiotic gametes are also useful in plant breeding.

A Stevia extract made from leaves of the Stevia rebaudiana plant is described. The extract has desired levels of steviol glycosides and is useful in food, beverage, and other consumable products.

Cosmos Bipinnatus cotyledons are isolated as explants, and the isolated explants are cultured onto a regeneration medium to obtain regenerated Cosmos Bipinnatus shoots. The regeneration medium optionally includes a basal medium, a cytokinin, an ethylene action inhibitor, and a nitrogen source.