The disclosure provides an aseptic sowing and raising seedling method for distant hybridization seeds of Phalaenopsis and Rhynchostylis retusa, which takes Phalaenopsis as female parent and Rhynchostylis retusa as male parent to obtain hybridization fruit pods by artificial pollination, the fruit pods are pretreated, and the seeds are aseptically sowed, germinated, protocorm proliferated and differentiated, strong seedlings rooted, acclimatized and transplanted to obtain distant hybridization offspring groups. The disclosure has the advantages of high seed germination rate, large seedling number, short seedling time and good seedling quality, which solves the problems that the seeds of Phalaenopsis distant hybridization process are difficult to succeed due to affinity, and are extremely difficult to germinate and raise seedlings under natural conditions. Therefore, a large number of seedlings can be obtained in a short period by aseptic sowing and artificial propagation, which lays a foundation for breeding excellent varieties of Phalaenopsis distant hybridization.

A Stevia extract made from leaves of the Stevia rebaudiana plant is described. The extract has desired levels of steviol glycosides and is useful in food, beverage, and other consumable products.

The present invention is directed to a transgenic plant and a method for producing the same. In particular, the present invention is directed to a transgenic plant or a plant cell in which a nucleic acid molecule encoding an m.sup.6A demethylase is introduced, wherein said m.sup.6A demethylase has the following two domains: i) N-terminal domain (NTD) having the function of AlkB oxidation demethylase; and ii) C-terminal domain (CTD). The present invention is also directed to a method for producing said plant, comprising introducing a nucleic acid molecule encoding an m.sup.6A demethylase into a regenerable plant cell, and regenerating a transgenic plant from the regenerable plant cell.

The present invention provides a longitudinally split immature tiller separated from a rhizome (LSITR), a cluster of multiple in vitro shoots (CMIT), a cluster of stem segments containing shoot primordia (CSSSP) and an in vitro tiller of Miscanthus x giganteus (Giant Miscanthus), and their uses in propagating Miscanthus x giganteus (Giant Miscanthus).

The present invention relates to a method for conducting high-throughput and directed mutagenesis for sugarcane resistance to glyphosate by plasma. The method is as follows: sugarcane embryonic calli are irradiated by a plasma instrument under a sterile condition for mutagenesis, wherein the mutagenesis power is 140˜200 W, the discharging distance is 35˜45 mm, the mutagenesis time is 110˜140 s and the protective gas is nitrogen; buffering culture, moderate/high concentration of glyphosate stress screening, differentiation into seedlings, glyphosate stress screening of bottle seedlings and stress screening via spraying glyphosate on the leave surfaces of potted plants are conducted for the treated calli. The present invention has the advantages of safe operation, simplicity, practicability, high handling capacity, low contamination, and due to implementation of directed stress screening, high screening efficiency, decreased subsequent screening workload and visual identification of resistant mutant strains.

The present application relates to methods for the production of plant material suitable for the extraction and purification of saponins, through plantations of clone plants of defined chemotype, including ultrahigh density plantations of Quillaja saponaria Molina, and methods to increase the recovery of saponins by solid/liquid extraction of the harvested plant material.

The present invention discloses and claims methods and devices for the rapid mechanical isolation of monocot plant tissues suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating target plant tissues for use as transformable explants, and propagation of transgenic plants and plant tissues.

The present invention is directed to a transgenic plant and a method for producing the same. In particular, the present invention is directed to a transgenic plant or a plant cell in which a nucleic acid molecule encoding an m.sup.6A demethylase is introduced, wherein said m.sup.6A demethylase has the following two domains: i) N-terminal domain (NTD) having the function of AlkB oxidation demethylase; and ii) C-terminal domain (CTD). The present invention is also directed to a method for producing said plant, comprising introducing a nucleic acid molecule encoding an m.sup.6A demethylase into a regenarable plant cell, and regenerating a transgenic plant from the regenerable plant cell.

Methods of in vitro clonal propagation, regeneration and transformation in Cannabis are provided. Also provided is the use of such methods in improvements of cannabis cultivars such as via breeding.

The present description relates to a plant cutting device for cutting or cloning a plant, and housing the plant for growth. The plant cutting device generally comprises two compartments that are operatively connected together and displaceable from an open position to a closed position defining an enclosure, a cutter mounted within at least one of the compartments and configured to cut a plant part when the first and second compartments are displaced to the closed position, a cut end of the plant part being accommodated within the enclosure; and a growth medium disposed in the first compartment and/or the second compartment for contacting the cut end of the plant part allowing for the growth of the plant.