The present invention includes long-day onion plants comprising bulbs having low pungency and methods for obtaining such onions. The present invention also provides reagents and materials that can be used in the methods for obtaining such onions.

Provided herein are materials and methods for producing diploid, fertile, uniform, and vigorous hybrid potato. Also provided are methods of using advanced breeding methods, such as genome design, to generate potato inbred lines with high homozygosity which enables the exploitation of heterosis in this tuber crop and transforms potato breeding from a slow, non-accumulative mode into a fast-iterative one.

Provided herein are materials and methods for producing diploid, fertile, uniform, and vigorous hybrid potato. Also provided are methods of using advanced breeding methods, such as genome design, to generate potato inbred lines with high homozygosity which enables the exploitation of heterosis in this tuber crop and transforms potato breeding from a slow, non-accumulative mode into a fast-iterative one.

The present invention provides a Chlorella variabilis-derived phosphomannose isomerase gene, herein named ChloPMI. The present invention also provides a prokaryotic expression vector comprising ChloPMI, which can be used for identifying mannose metabolic activity of ChloPMI protein. Further, the present invention provides an expression cassette and a plant expression vector comprising ChloPMI, and a use of the expression cassette and the expression vector in genetic transformation of plants. According to the present invention, the transformation of rice cells is successfully achieved with the plant expression vector constructed from the ChloPMI gene using mannose as a selection agent. According to the present invention, a plant-derived phosphomannose isomerase gene is successfully separated and cloned from Chlorella variabilis. Since the plant-derived phosphomannose isomerase gene is derived from Chlorella variabilis, it is environment-friendly and has no potential hazard to human, which is very beneficial in promoting and applying transgenic products and eliminating any existing doubts on transgenes.

The present invention includes long-day onion plants comprising bulbs having low pungency and methods for obtaining such onions. The present invention also provides reagents and materials that can be used in the methods for obtaining such onions.

The present invention relates to non-transgenic and transgenic plants, preferably crop plants, comprising a mutation causing an alteration of the amino acid sequence in the CATD domain of the centromere histone H3 (CENH3), preferably within the loop1 or the α2-helix of the CATD domain, which have the biological activity of a haploid inducer. Further, the present invention provides methods of generating the plants of the present invention and haploid and double haploid plants obtainable by crossing the plants of the present invention with wildtype plants as well as methods of facilitating cytoplasm exchange.

The present invention relates to non-transgenic and transgenic plants, preferably crop plants, comprising a mutation causing an alteration of the amino acid sequence in the CATD domain of the centromere histone H3 (CENH3), preferably within the loop1 or the α2-helix of the CATD domain, which have the biological activity of a haploid inducer. Further, the present invention provides methods of generating the plants of the present invention and haploid and double haploid plants obtainable by crossing the plants of the present invention with wildtype plants as well as methods of facilitating cytoplasm exchange.

Cloning and use of an Arachis hypogaea L. flowering habit gene AhFH1 and allelic variants thereof are provided. Through experiments, the Arachis hypogaea L. flowering habit gene AhFH1 and two defunctionalized allelic variants thereof are determined. The defunctionalized allelic variants can cause the change from alternate flowering Arachis hypogaea L. to continuous flowering Arachis hypogaea L. Through overexpression of the gene AhFH1 or supplementary expression of a promoter of the gene itself, a continuous flowering Arachis hypogaea L. variety can be changed into alternate flowering Arachis hypogaea L.; and through knockout or expression suppression of the gene AhFH1, alternate flowering Arachis hypogaea L. can be changed into continuous flowering Arachis hypogaea L. Marker-assisted selection (MAS) breeding is realized for allelic variants of the gene using molecular markers.

Provided are the DNA of a glycoalkaloid biosynthetic enzyme of solanaceous plants (Solanaceae) such as potato, solanaceous plants producing no glycoalkaloids, and a method of producing a cultivar with a reduced risk of accumulating glycoalkaloids, which involves crossing plants in which the expression of an oxidase gene involved in glycoalkaloid biosynthesis is suppressed or the activity of the enzyme is lowered, as mother plants, and screening progeny resulting from the crossing.

Provided are the DNA of a glycoalkaloid biosynthetic enzyme of solanaceous plants (Solanaceae) such as potato, solanaceous plants producing no glycoalkaloids, and a method of producing a cultivar with a reduced risk of accumulating glycoalkaloids, which involves crossing plants in which the expression of an oxidase gene involved in glycoalkaloid biosynthesis is suppressed or the activity of the enzyme is lowered, as mother plants, and screening progeny resulting from the crossing.