A hierarchical catalyst composition comprising a continuous or particulate macroporous scaffold in which is incorporated mesoporous aggregates of magnetic nanoparticles, wherein an enzyme is embedded in mesopores of the mesoporous aggregates of magnetic nanoparticles. Methods for synthesizing the hierarchical catalyst composition are also described. Also described are processes that use the recoverable hierarchical catalyst composition for depolymerizing lignin, remediation of water contaminated with aromatic substances, polymerizing monomers by a free-radical mechanism, epoxidation of alkenes, halogenation of phenols, inhibiting growth and function of microorganisms in a solution, and carbon dioxide conversion to methanol. Further described are methods for increasing the space time yield and/or total turnover number of a liquid-phase chemical reaction that includes magnetic particles to facilitate the chemical reaction, the method comprising subjecting the chemical reaction to a plurality of magnetic fields of selected magnetic strength, relative position in the chemical reaction, and relative motion.

A composition for removing spillage on asphalt paving of hydrocarbon substances which includes modifiers, chemical oxidizers and bacteria which metabolizes both the spillage and a softened top layer of asphalt paving to aid in rehardening the surface of the mat while not metabolizing the underlying asphalt paving unaffected by the spillage.

A composition for removing spillage on asphalt paving of hydrocarbon substances which includes modifiers, chemical oxidizers and bacteria which metabolizes both the spillage and a softened top layer of asphalt paving to aid in rehardening the surface of the mat while not metabolizing the underlying asphalt paving unaffected by the spillage.

Methods to remediate a contaminated material are provided. In one embodiment, a biocatalyst that digests hydrocarbon contaminants is activated with a nutrient and the activated biocatalyst is combined with the contaminated material and water to form a mixture. The mixture is incubated for a period of time, and the level of contaminant in the mixture is determined to ascertain whether to incubate further, add additional biocatalyst mix, or provide the remediated material for further processing. In one embodiment, the remediated material is provided for reuse or recycling with a second material, such as a construction aggregate. The method is particularly suited for remediation of drill cuttings, mine tailings, hydrocarbon-contaminated soil, and the like.

Methods to remediate a contaminated material are provided. In one embodiment, a biocatalyst that digests hydrocarbon contaminants is activated with a nutrient and the activated biocatalyst is combined with the contaminated material and water to form a mixture. The mixture is incubated for a period of time, and the level of contaminant in the mixture is determined to ascertain whether to incubate further, add additional biocatalyst mix, or provide the remediated material for further processing. In one embodiment, the remediated material is provided for reuse or recycling with a second material, such as a construction aggregate. The method is particularly suited for remediation of drill cuttings, mine tailings, hydrocarbon-contaminated soil, and the like.

The present invention discloses a strain of Achromobacter xylosoxidans with monomethylamine degradability and the application thereof. This strain, named Achromobacter xylosoxidans GDUTAN5, was deposited on May 24, 2017 in the China Center for Type Culture Collection in Wuhan University (No. 299 Bayi Road, Wuchang District, Wuhan City, Hubei Province) with a deposit number of CCTCC NO: M 2017285. This Achromobacter xylosoxidans GDUTAN5 was Gram-negative and rod-shaped, and the colony appeared to be round, light yellow, opaque and smooth, having a diameter of 1-2 mm. The Achromobacter xylosoxidans GDUTAN5 of the present invention can be applied to environmental restoration, degrading monomethylamine in the environment at a high degradation efficiency. When it degraded monomethylamine for 96 h at a substrate concentration of 5 mg/L, the degradation efficiency could reach 92.3%.

Methods to remediate a contaminated material are provided. In one embodiment, a biocatalyst that digests hydrocarbon contaminants is activated with a nutrient and the activated biocatalyst is combined with the contaminated material and water to form a mixture. The mixture is incubated for a period of time, and the level of contaminant in the mixture is determined to ascertain whether to incubate further, add additional biocatalyst mix, or provide the remediated material for further processing. In one embodiment, the remediated material is provided for reuse or recycling with a second material, such as a construction aggregate. The method is particularly suited for remediation of drill cuttings, mine tailings, hydrocarbon-contaminated soil, and the like.

Methods to remediate a contaminated material are provided. In one embodiment, a biocatalyst that digests hydrocarbon contaminants is activated with a nutrient and the activated biocatalyst is combined with the contaminated material and water to form a mixture. The mixture is incubated for a period of time, and the level of contaminant in the mixture is determined to ascertain whether to incubate further, add additional biocatalyst mix, or provide the remediated material for further processing. In one embodiment, the remediated material is provided for reuse or recycling with a second material, such as a construction aggregate. The method is particularly suited for remediation of drill cuttings, mine tailings, hydrocarbon-contaminated soil, and the like.

The present disclosure provides a method for preparing an organophosphorus degrading enzyme based multifunctional catalyst and an organophosphorus degrading enzyme based multifunctional catalyst and use thereof. In the present disclosure, the preparation method includes: directly adding a composite yolk-shell-structured nanomaterial into a crude enzyme solution of organophosphorus degrading enzyme with an affinity tag, and mixing, to obtain a mixture, and then subjecting the mixture to a separation, to obtain an organophosphorus degrading enzyme based multifunctional catalyst. According to the present disclosure, the method for preparing an organophosphorus degrading enzyme based multifunctional catalyst is simple in operation, and has a low cost; the multifunctional catalyst prepared by the same has low requirement for the purity of enzyme, support of which could be directionally binded with enzyme, and could be used for detecting an organophosphorus pesticide, and also for a cascade degradation of an organophosphorus pesticide. The final product p-aminophenol has important application value.

The present invention provides tools and methods for degrading MTBE, TBA and/or HCHO using abacierial consortium comprising one or more strains selected from Methylibium LD3, Hydrogenophaga LD1 and/or Mycobacterium LD6.