A portable, ex vivo perfusion system for preserving detached biological tissue includes a receptacle for housing the tissue in a normothermic environment, a perfusion core to pump perfusate through the tissue via at least one conduit, at least one detection device to measure parameters during perfusion, and at least one parameter control device to maintain the parameter in a predetermined threshold. The system also include a controller with instructions to receive the measured parameters, compare the parameters to predetermined thresholds, and when the parameters are outside the thresholds change an output of the at least one parameter control device to get the parameters within the threshold and alert a user that parameters were outside the thresholds.

A portable, ex vivo perfusion system for preserving detached biological tissue includes a receptacle for housing the tissue in a normothermic environment, a perfusion core to pump perfusate through the tissue via at least one conduit, at least one detection device to measure parameters during perfusion, and at least one parameter control device to maintain the parameter in a predetermined threshold. The system also include a controller with instructions to receive the measured parameters, compare the parameters to predetermined thresholds, and when the parameters are outside the thresholds change an output of the at least one parameter control device to get the parameters within the threshold and alert a user that parameters were outside the thresholds.

The disclosure provides for electrode systems and perfusion systems that may be configured to measure the electrical activity of an explanted heart and to provide defibrillation energy as necessary. The perfusion systems may maintain the heart in a beating state at, or near, normal physiological conditions; circulate oxygenated, nutrient enriched perfusion fluid to the heart at or near physiological temperature, pressure, and/or flow rate. These systems may include a pair of electrodes that may be placed epicardially on the right atrium and/or left ventricle of the explanted heart, and/or an electrode placed in the aortic blood path.

The disclosure provides for electrode systems and perfusion systems that may be configured to measure the electrical activity of an explanted heart and to provide defibrillation energy as necessary. The perfusion systems may maintain the heart in a beating state at, or near, normal physiological conditions; circulate oxygenated, nutrient enriched perfusion fluid to the heart at or near physiological temperature, pressure, and/or flow rate. These systems may include a pair of electrodes that may be placed epicardially on the right atrium and/or left ventricle of the explanted heart, and/or an electrode placed in the aortic blood path.

A normothermic machine perfusion method for a pancreas is provided. First, a perfusate contained in a venous reservoir may be oxygenated such that a ratio of oxygen to carbon dioxide in the oxygenated perfusate is approximately 90%/10%. A first portion of the oxygenated perfusate may be pumped from the venous reservoir, through a dialysis filter, and back to the venous reservoir. A dialysate comprising a salt and glucose may be infused into the first portion of the oxygenated perfusate as the first portion is pumped through the dialysis filter. A concentration of glucose in the dialysate may be less than 8 mmol/L. A second portion of the oxygenated perfusate may be pumped through an arterial filter and through a pancreas graft. Venous outflow from the pancreas graft may then be pumped to the venous reservoir.

A normothermic machine perfusion method for a pancreas is provided. First, a perfusate contained in a venous reservoir may be oxygenated such that a ratio of oxygen to carbon dioxide in the oxygenated perfusate is approximately 90%/10%. A first portion of the oxygenated perfusate may be pumped from the venous reservoir, through a dialysis filter, and back to the venous reservoir. A dialysate comprising a salt and glucose may be infused into the first portion of the oxygenated perfusate as the first portion is pumped through the dialysis filter. A concentration of glucose in the dialysate may be less than 8 mmol/L. A second portion of the oxygenated perfusate may be pumped through an arterial filter and through a pancreas graft. Venous outflow from the pancreas graft may then be pumped to the venous reservoir.

Systems comprising a microfluidic device are provided for maintaining and analyzing tissue slices. Methods for maintaining tissue slices in a microfluidic device are further provided.

Systems comprising a microfluidic device are provided for maintaining and analyzing tissue slices. Methods for maintaining tissue slices in a microfluidic device are further provided.

A system for the hypothermic transport of biological samples, such as tissues, organs, or body fluids. The system includes a self-purging preservation apparatus to suspend a sample in preservation fluid and perfuse a tissue with preservation fluid. The self-purging preservation apparatus is placed in an insulated transport container having a cooling medium. When assembled, the system allows for transport of biological samples for extended periods of time at a stable temperature.

A system for the hypothermic transport of biological samples, such as tissues, organs, or body fluids. The system includes a self-purging preservation apparatus to suspend a sample in preservation fluid and perfuse a tissue with preservation fluid. The self-purging preservation apparatus is placed in an insulated transport container having a cooling medium. When assembled, the system allows for transport of biological samples for extended periods of time at a stable temperature.