Systems and methods herein generally relate to prolonging viability of bodily tissue, especially an organ, by adjusting pressure as needed to maintain a constant pressure within the organ even during external pressure fluctuations due, for example, to transportation of the organ in an airplane. The systems and methods herein can include an electronic pump that pumps gas into an organ and a mechanical pressure regulator to release gas based on organ pressure.

A system and method for maintaining an oxygen concentration of a biological sample. The oxygen concentration can be maintained by measuring the oxygen concentration within the biological sample and adjusting a rate of an oxygen supplier in response to this measurement. For example, when the oxygen concentration is below a threshold, oxygen can be delivered to the biological sample at a higher rate.

The systems and methods relate to cyclically inflating and deflating a lung. The lung can be stored and transported while pumping a gas into the lung and removing another gas from the lung. The cyclical inflation and deflation of the lung can be performed at a rate enhanced or optimized for lung performance.

Methods and systems provide a human kidney perfusion model with dual blood supply channels, permitting two kidneys from the same donor to be pumped simultaneously for rigorously controlled therapeutic testing.

Methods and systems provide a human kidney perfusion model with dual blood supply channels, permitting two kidneys from the same donor to be pumped simultaneously for rigorously controlled therapeutic testing.

This disclosure is related to methods of preserving biological samples such as organs, and tissue. For example, the disclosure provides methods for preservation of a biological sample. The methods can comprise providing a system, placing the biological sample into a reservoir, and causing the computer control unit to perform operations.

A perfusion loop assembly for ex vivo liver perfusion includes a pump providing perfusion fluid through a line branching at a branching point into a first branch line and a second branch line. The first branch line provides a first portion of the perfusion fluid to the hepatic artery of the liver, the first branch line coupled with a gas exchanger, where the first branch line includes a flow rate sensor and/or a pressure sensor. The second branch line provides a second portion of the perfusion fluid to the portal vein of the liver; the second branch line includes a valve for controlling flow of perfusion fluid into the portal vein. The second branch line includes a flow rate sensor and/or a pressure sensor. A liver chamber assembly holds the liver ex vivo, and an outlet line for the perfusion fluid connects the liver chamber assembly and the pump.

A perfusion loop assembly for ex vivo liver perfusion includes a pump providing perfusion fluid through a line branching at a branching point into a first branch line and a second branch line. The first branch line provides a first portion of the perfusion fluid to the hepatic artery of the liver, the first branch line coupled with a gas exchanger, where the first branch line includes a flow rate sensor and/or a pressure sensor. The second branch line provides a second portion of the perfusion fluid to the portal vein of the liver; the second branch line includes a valve for controlling flow of perfusion fluid into the portal vein. The second branch line includes a flow rate sensor and/or a pressure sensor. A liver chamber assembly holds the liver ex vivo, and an outlet line for the perfusion fluid connects the liver chamber assembly and the pump.

A method for controlling at least one pump in a perfusion apparatus for delivering a fluid to at least one organ through a plurality of vessels for maintaining the viability of the at least one organ, the method including supplying a fluid to a first vessel of an organ and to a second vessel of an organ; measuring a first parameter of the fluid flowing in the first vessel and a second parameter of the fluid flowing in the second vessel; and executing direct control of the second parameter of the fluid flowing in the second vessel to influence the first parameter of the fluid flowing in the first vessel.

A cannula includes a first circumferential portion, a second circumferential portion, and a seal with a first clamping surface. The first circumferential portion and the second circumferential portion are configured to mutually cooperate to support a circumference of vasculature, and form a second clamping surface. The first clamping surface and the second clamping surface are configured to cooperate to secure an end of the vasculature.