One aspect of the invention provides a method for preparing a vein graft. The method includes: applying a tissue passivation agent to a resected anatomical vessel; placing the resected anatomical vessel in a chamber; and allowing the tissue passivation agent to cross-link while the resected anatomical vessel is in the chamber.

Provided herein are systems, methods, and cryoprotective solutions for reversible cryopreservation of biological specimens, whole organs, and whole organisms. Exemplary methods include loading a cryoprotective agent into the biological specimen, cooling the biological specimen to a cryogenic temperature for preservation, storing the biological specimen at a preservation temperature state to preserve the biological specimen, rewarming the biological specimen by increasing a temperature of the biological specimen above the preservation temperature state, and unloading the cryoprotective agent from the biological specimen. The cooling is performed at a first rate to reduce ice formation, substantially homogeneously to reduce propensity for cracking of the preserved biological specimen, and at a first pressure to prevent or reduce ice expansion within the preserved biological specimen. The rewarming is performed at a second rate to reduce ice formation, substantially homogeneously to reduce propensity for cracking, and at a second pressure to prevent or reduce ice expansion.

Provided herein are systems, methods, and cryoprotective solutions for reversible cryopreservation of biological specimens, whole organs, and whole organisms. Exemplary methods include loading a cryoprotective agent into the biological specimen, cooling the biological specimen to a cryogenic temperature for preservation, storing the biological specimen at a preservation temperature state to preserve the biological specimen, rewarming the biological specimen by increasing a temperature of the biological specimen above the preservation temperature state, and unloading the cryoprotective agent from the biological specimen. The cooling is performed at a first rate to reduce ice formation, substantially homogeneously to reduce propensity for cracking of the preserved biological specimen, and at a first pressure to prevent or reduce ice expansion within the preserved biological specimen. The rewarming is performed at a second rate to reduce ice formation, substantially homogeneously to reduce propensity for cracking, and at a second pressure to prevent or reduce ice expansion.

Provided are systems and methods for treating a biological fluid, e.g., to inactivate pathogens.

Provided are systems and methods for treating a biological fluid, e.g., to inactivate pathogens.

A device for controllable growth of ice crystals inside an organic object during freezing, the device comprising at least one transducer, a control unit, a power supply and a modulator, wherein the control unit causes a plurality of types of magnetic fields to be emitted, by the at least one transducer, in a cyclical manner, and the control unit determines, for each type of magnetic field comprised in the plurality of types of magnetic fields, a carrier frequency, a modulation frequency, modulation boundaries and duty cycle, and sets them by utilizing the signal generator and the power supply; and wherein a strength of the plurality of the types of the magnetic fields is determined by adjusting an output voltage provided by the power supply.

A device for controllable growth of ice crystals inside an organic object during freezing, the device comprising at least one transducer, a control unit, a power supply and a modulator, wherein the control unit causes a plurality of types of magnetic fields to be emitted, by the at least one transducer, in a cyclical manner, and the control unit determines, for each type of magnetic field comprised in the plurality of types of magnetic fields, a carrier frequency, a modulation frequency, modulation boundaries and duty cycle, and sets them by utilizing the signal generator and the power supply; and wherein a strength of the plurality of the types of the magnetic fields is determined by adjusting an output voltage provided by the power supply.

A system and methodology for the preservation of red blood cells is described in which red blood cells are oxygen or oxygen and carbon dioxide depleted, treated and are stored in an anaerobic environment to optimize preparation for transfusion. More particularly, a system and method for extended storage of red blood cells from collection to transfusion that optimizes red blood cells prior to transfusion is described.

A system and methodology for the preservation of red blood cells is described in which red blood cells are oxygen or oxygen and carbon dioxide depleted, treated and are stored in an anaerobic environment to optimize preparation for transfusion. More particularly, a system and method for extended storage of red blood cells from collection to transfusion that optimizes red blood cells prior to transfusion is described.

A method for preserving a tissue graft includes submerging the tissue graft in a solution containing one or more antimicrobial and/or stabilizing agents and storing the tissue graft in the solution for a period of time of at least 24 hours while the solution is in an unfrozen state. A tissue preservation system includes a solution containing one or more antimicrobial and/or stabilizing agents and a tissue graft in the solution.