Techniques Nuc-seq, Div-Seq, and Dronc-Seq are allow for unbiased analysis of any complex tissue. Nuc-Seq, a scalable single nucleus RNA-Seq method, can sensitively identify closely related cell types, including within the adult hippocampus. Div-seq combines Nuc-Seq with EdU-mediated labeling of proliferating cells, allowing tracking of transcriptional dynamics of newborn neurons in an adult neurogenic region in the hippocampus. Dronc-Seq uses a microfluidic device to co-encapsulate individual nuclei in reverse emulsion aqueous droplets in an oil medium together with one uniquely barcoded mRNA-capture bead.

A culture medium and a method to efficiently grow neuronal stem cells. The culture medium includes FGF-2, EGF, and a proteoglycan, where the proteoglycan is preferably a chondroitin sulfate-type proteoglycan, and more preferably, a salmon nasal cartilage-derived proteoglycan. The culture medium is capable of promoting formation of neurospheres.

This disclosure describes methods of preparing a decellularized Descemet's membrane and an isolated Descemet's membrane, methods of using an isolated Descemet's membrane, and tissues prepared using an isolated Descemet's membrane. This disclosure further describes a composition that includes an isolated Descemet's membrane. In some embodiments, the tissues and methods described herein may be used to treat a limbal stem cell deficiency or as an ocular surface bandage.

A cell preparation for treating brain tumors used in combination with a prodrug that is converted to 5-fluorouracil by cytosine deaminase, wherein the cell preparation comprises neural stem cells derived from pluripotent stem cells having a cytosine deaminase gene and a uracil phosphoribosyltransferase gene is provided to establish new means for treating brain tumors.

Disclosed herein is a 4D printed programmable culture substrate with the self-morphing ability to accommodate the change in morphology of stem cells during differentiation. The 4D printed culture substrate includes a shape memory polymer that is configured for transformation from a first topographical shape to a second topographical shape during a predetermined time period in response to a stimulus, such as temperature. The first topographical shape may include micro-wells and the second topographical shape may include microgrooves, which can accommodate the growth and differentiation of neural stem cells.

The present disclosure relates to a cryopreserved pharmaceutical composition comprising immature dental pulp stem cells (IDPSCs) expressing SOX-1 and SOX-2 and methods of treating a neurological disease or condition comprising systemically administering to a subject a cryopreserved pharmaceutical composition comprising IDPSCs expressing SOX-1 and SOX-2.

A method for culturing cells on a substrate capable of inducing pluripotency is provided. The method includes plating cells on a nichoid-type substrate, allowing cultured cells to proliferate for a certain period of time, detaching cells from the nichoid-type substrate, and, once cells have been detached, culturing cells in suspension or under adhesion.

Human pluripotent stem cells are differentiated in vitro into forebrain subdomain structures, which are then fused to generate an integrated system for use in analysis, screening programs, and the like.

The disclosure relates to oligodendrocyte-biased glial progenitor cells and methods of making, isolating, and using such cells.

The present invention relates to a composition for preserving cells and a method for preserving cells and, more specifically, to: a composition for preserving cells, containing, as active ingredients, plant-derived recombinant human serum albumin and plant peptides, wherein the composition maintains a high cellular survival rate while maintaining animal-free and xeno-free properties and is stable without changes in cellular morphology or surface expression factors in the short-term preservation of cells such as stem cells or primary cultured cells; and a method for preserving cells by using the same.