A method for promoting the accumulation of cannabinoid substances is disclosed. The method comprises the step of adding an irradiation of green light, which has a peak wavelength at 505-526 nm, into the indoor growing environment of cannabis to improve the levels of tetrahydrocannabinol (THC) and cannabidiol (CBD), cannabinoid substances in cannabis. While maintaining the light intensity and other growth conditions, the yields and/or levels of THC and CBD, cannabinoid substances in cannabis, can be increased by up to 21.35%.

A method for promoting the accumulation of cannabinoid substances is disclosed. The method comprises the step of adding an irradiation of green light, which has a peak wavelength at 505-526 nm, into the indoor growing environment of cannabis to improve the levels of tetrahydrocannabinol (THC) and cannabidiol (CBD), cannabinoid substances in cannabis. While maintaining the light intensity and other growth conditions, the yields and/or levels of THC and CBD, cannabinoid substances in cannabis, can be increased by up to 21.35%.

Methods to grow cannabis plants within an interior of an enclosure are described, the method comprises condensing water vapor from the interior of the enclosure to produce a source of liquid water and supplying the liquid water to the cannabis plants. The source of liquid water may be supplied to a common reservoir and then transferred to the cannabis plants. The common reservoir includes fish, a microorganism, or treated water. Water drained from the cannabis plants may be recycled back to the common reservoir. The water may be filtered or oxygenated and mixed with a pH adjustment solution, a macro-nutrient, a micro-nutrient, a carbohydrate, an enzyme, or a vitamin. Solar panels may be used to provide electricity for electrically powered lights that illuminate the cannabis plants. The cannabis plants may be grown within a growing medium, harvested, trimmed, ground, heated, and made into multifunctional compositions or foodstuffs.

Methods to grow cannabis plants within an interior of an enclosure are described, the method comprises condensing water vapor from the interior of the enclosure to produce a source of liquid water and supplying the liquid water to the cannabis plants. The source of liquid water may be supplied to a common reservoir and then transferred to the cannabis plants. The common reservoir includes fish, a microorganism, or treated water. Water drained from the cannabis plants may be recycled back to the common reservoir. The water may be filtered or oxygenated and mixed with a pH adjustment solution, a macro-nutrient, a micro-nutrient, a carbohydrate, an enzyme, or a vitamin. Solar panels may be used to provide electricity for electrically powered lights that illuminate the cannabis plants. The cannabis plants may be grown within a growing medium, harvested, trimmed, ground, heated, and made into multifunctional compositions or foodstuffs.

A method of increasing the cannabinoid levels in a genetically modified Cannabis sativa plant includes genetically modifying the plant to induce the overexpression of the gene that controls the expression of tetrahydrocannabinolic acid (THCA) synthase and/or cannabidiolic acid (CBDA) synthase. The overexpression of THCA synthase and CBDA synthase catalyzes an increased synthesis of cannabigerolic acid to tetrahydrocannabinolic acid and cannabidiolic acid, as well as the cannabinoids (3aR)-2,4,4-trimethyl-7-pentyl-3,3a,4,9b-tetrahydrocyclopenta[c]chromen-9-ol and 2-((1R,5R)-3-methyl-5-(prop-1-en-2-yl)cyclopent-2-en-1-yl)-5-pentylbenzene-1,3-diol. Pharmaceutical compositions comprising the modified cannabinoids produced by the transgenic Cannabis sativa plant or prepared synthetically are used to treat various diseases and conditions.

The present disclosure relates to methods of determining the terpene profile of cannabis plant material, specifically through use of gas chromatography-mass spectrometry (GC-MS). Extraction methods include liquid based extraction methods, specifically hexane based liquid extraction methods, and headspace extraction methods, specifically static headspace extraction and headspace solid phase microextraction (HS-SPME). Static headspace and HS-SPME are shown to be better for monoterpene extraction, and hexane based liquid extraction for sesquiterpene extraction.

The present invention discloses a modified Cannabis plant exhibiting at least one improved domestication trait compared with wild type Cannabis, wherein the modified plant comprises at least one mutated Cannabis SELF PRUNING (SP) (CsSP) gene and/or at least one mutated Cannabis SELF PRUNING 5G (SP5G) (CsSP5G) gene. The present invention further discloses methods for production of the aforementioned modified Cannabis plant using genome modification.

A plant propagation system, process and method are provided for promoting the growth of plant tissue into propagules using a photoautotrophic gel system. The plant propagation system includes a sterile growth vessel that has a vented lid to permit passive diffusion of gases. The process is initiated with one or more sterile rooted explants, which are then cultured in a large container with a vented lid photoautotrophically, which simulates ex-vitro growth conditions. These nodal explants can then be rooted onto photoautotrophic rooting agar gel in vented lid containers and subsequently transferred onto a substrate of choice for mature growth ex-vitro.

A plant propagation system, process and method are provided for promoting the growth of plant tissue into propagules using a photoautotrophic gel system. The plant propagation system includes a sterile growth vessel that has a vented lid to permit passive diffusion of gases. The process is initiated with one or more sterile rooted explants, which are then cultured in a large container with a vented lid photoautotrophically, which simulates ex-vitro growth conditions. These nodal explants can then be rooted onto photoautotrophic rooting agar gel in vented lid containers and subsequently transferred onto a substrate of choice for mature growth ex-vitro.

The present invention includes systems, methods and compositions for increasing trichome formation and/or density in cannabinoid producing plants, such as Cannabis and hemp. Additional aspects of the invention further include systems, methods and compositions for increasing cannabinoid and terpene biosynthetic and storage capacity in Cannabis.