A system and method for sorting sperm is provided. The system includes a housing and a microfluidic system supported by the housing. The system also includes an inlet providing access to the microfluidic system to deliver sperm to the microfluidic system and an outlet providing access to the microfluidic system to harvest sorted sperm from the microfluidic system. The microfluidic system provides a flow path for sperm from the inlet to the outlet and includes at least one channel extending from the inlet to the outlet to allow sperm delivered to the microfluidic system through the inlet to progress along the flow path toward the outlet. The microfluidic system also includes a filter including a first plurality of micropores arranged in the flow path between the inlet and the outlet to cause sperm traveling along the flow path to move against through the filter and gravity to reach the outlet.

Method for producing mobility in immobile cells in in vivo or in vitro fertilization. The method includes producing mobility in individual immobile cells that are connected to a microstructure. The microstructure is composed at least partially of magnetic materials and a non-reciprocal movement of the microstructure with the immobile cell is executed by a time-varying, three-dimensional external magnetic field.

Disclosed is a composition for selective enrichment of progressively motile non-fragmented/intact DNA sperms during swim-up of semen sample comprising combination of electrolytes, a mix of excipients, polyyvinylpyrrolidone, recombinant human serum albumin or bovine serum albumin. A method of selective enrichment of DNA-intact sperms using the compositions of the present invention is also disclosed. The DNA fragmentation index of the swim-up sperms are less than the DNA fragmentation index of the neat samples. The media compositions of the present invention are applicable for enrichment of non-fragmented/intact DNA sperms for therapeutic purposes in the field of assisted reproductive technologies.

Disclosed are methods and products for improving the quality of sperm, methods for treating subjects to improve sperm quality or improve fertility, and use of sperm with improved quality in assisted reproductive technologies, including exposing the sperm to BGP-15 and/or a derivative thereof, such as BGP-15, propanolol, bimoclomol, arimoclomal, NG94, iroxanadine, and/or a pharmaceutically acceptable derivative, prodrug, solvate, salt, tautomer, stereoisomer, and/or racemate thereof.

The disclosure relates to methods and systems for isolating individual sperm from a semen sample using microfluidics. Examples include a microfluidics system for isolating individual sperm from a semen sample, the system including: a first laminar flow channel extending between a first inlet and a first outlet; a second laminar flow channel extending between a second inlet and a second outlet; and a third laminar flow channel extending between the first and second laminar flow channels and having a restriction configured to prevent passage of a single sperm through the third laminar flow channel, wherein flow of a semen sample through the first laminar flow channel at a pressure higher than flow of a liquid medium through the second laminar flow channel results in a single sperm being trapped by the restriction in the third laminar flow channel.

The disclosure relates to Bovine germplasm of Bos taurus variety JE840003146074527. Included in the present disclosure are cells comprising the Bovine variety JE840003146074527. Also provided by the present disclosure are tissue cultures of cells, animals obtained from said cells, and parts thereof, including F1 spermatozoa. The disclosure further provides for methods of breeding, selecting, and using the germplasm to improve existing commercial cattle herds generated from in vitro fertilization methods and progeny cattle obtained from in vitro fertilization and implantation and artificial insemination methods.

The present invention relates to in vitro methods of inducing gametogenesis by producing meiotically competent cells. Reagents and kits for use in the methods of the invention are also provided. The present invention finds use in the field of medicine, particularly in the study and treatment of infertility.

The present invention relates to the separation of sperm. In particular, the present invention relates to the use of polyvinyl alcohol membranes in the electrophoretic separation of sperm.

The present disclosure provides, inter alia, methods to determine sperm quality and suitability of a donor' sperm for a reproduction modality. The present disclosure also provides methods to identify suitable reproduction modality for a sperm sample. The methods provided herein are amenable for screening sperm samples for use in assisted fertilization. The disclosure additionally provides articles of manufacture for performing the methods provided herein. The methods provided by the disclosure, in some embodiments, entail energy depletion with subsequent staged reintroduction of different energy sources.

System and method includes a body having a central microchannel separated by one or more porous membranes. The membranes are configured to divide the central microchannel into a two or more parallel central microchannels, wherein one or more first fluids are applied through the first central microchannel and one or more second fluids are applied through the second or more central microchannels. The surfaces of each porous membrane can be coated with cell adhesive molecules to support the attachment of cells and promote their organization into tissues on the upper and lower surface of the membrane. The pores may be large enough to only permit exchange of gases and small chemicals, or to permit migration and transchannel passage of large proteins and whole living cells. Fluid pressure, flow and channel geometry also may be varied to apply a desired mechanical force to one or both tissue layers.