Provided herein, inter alia, are PD-L1(+) natural killer cells that express soluble IL-15, PD-L1(+) natural killer cells that express soluble IL-15 and truncated EGFR, and methods of treating cancer using the PD-L1(+) natural killer cells. In an aspect is provided a method of treating cancer in a patient in need thereof comprising administering to the patient an effective amount of PD-L1(+) natural killer cells that express soluble IL-15.

Described are immune cells expressing a chimeric antigen receptor targeted to EGFR and having an oxygen-dependent degradation domain. The chimeric antigen receptor is co-expressed in immune cells, e.g., NK cells with a soluble form of human IL-15. The inclusion of an oxygen-dependent degradation domain substantially restricts the activity of the immune cells expressing the chimeric antigen receptor to the more hypoxic environment of solid tumor tissue. The NK cells expressing the chimeric antigen receptor are useful for treating breast cancer that has low or no HER2 expression.

The present invention relates to the field of biomedicine, and in particular, the present invention relates to an antibody or antigen-binding fragment thereof which specifically binds to MSLN, and a chimeric antigen receptor (CAR) comprising said antibody or antigen-binding fragment thereof. The present invention also relates to modified immune cells expressing said CAR, or co-expressing said CAR and additional bioactive molecules (e.g. PD-1 antibody and/or mIL-15), and a method for preparing said modified immune cells. The present invention also relates to the use of such antibodies, CARs, and immune cells for the prevention and/or treatment of diseases associated with the expression of mesothelin, such as malignant pleural mesothelioma, pancreatic cancer, lung cancer, breast cancer, and ovarian cancer, and a method for the prevention and/or treatment of diseases associated with the expression of mesothelin, such as malignant pleural mesothelioma, pancreatic cancer, lung cancer, breast cancer, ovarian cancer, and other MSLN-positive tumors.

Provided are nucleic acids that encode decoy-resistant interleukin 18 (DR-18) polypeptides, as well as vectors and cells comprising such nucleic acids and cells that comprise such vectors. Cells encoding DR-18 polypeptides may be referred to as DR-18 armored cells. The nucleic acids may further encode a chimeric antigen receptor (CAR) or multiple CARs, including CARs that bind to antigens described herein. Methods are also provided, including methods of making the nucleic acids, vectors, and/or cells, as well as methods of use, such as employing the nucleic acids, vectors, and/or cells, in the treatment of a subject having cancer.

The present invention relates to a targeting module comprising at least one tumor-binding domain, in particular at least one IL13R2-binding domain and/or at least one HER2-binding domain, and a tag-binding domain or a tag for use in a method for stimulating a chimeric antigen receptor-mediated immune response in a mammal, a nucleic acid, a vector or a cell comprising a nucleotide sequence encoding the targeting module, a pharmaceutical composition and a kit comprising the targeting module and a vector or a cell comprising a nucleotide sequence encoding a switchable chimeric antigen receptor.

Disclosed herein are recombinant nucleic acids, comprising a 5 untranslated (5-UTR) sequence portion, a signal peptide sequence portion, a single chain antibody fragment sequence portion, a hinge region sequence portion, a transmembrane domain sequence portion, and one or more intracellular domain sequence portions. Also disclosed herein are modified natural killer (NK) cells comprising the recombinant nucleic acid described above. Further disclosed herein are methods of treating a tumor in a subject by administering the modified NK cells.

The present invention belongs to the field of biomedicine. Specifically, it provides an antibody targeting CCR8 and its applications. More particularly, the present invention offers an antibody targeting CCR8, STAR and CAR derived from the antibody, therapeutic immune cells containing the STAR or CAR, and their applications in disease treatment.

The present invention belongs to the field of biomedicine. Specifically, the present invention provides an antibody targeting Claudin18.2 and its applications. More specifically, the present invention provides an antibody targeting Claudin18.2, a STAR derived from the antibody, therapeutic immune cells containing the STAR, and their applications in the treatment of diseases.

Provided is an improved method for preparing tumor-infiltrating lymphocytes, the method comprising removing Treg cells that inhibit the activity of effector T cells or converting Treg cells into CD8.sup.+ effector T cells, whereby the tumor-infiltrating lymphocytes have excellent ability to kill tumor cells and excellent proliferative ability.

Transgenic T cells and vectors for making transgenic T cells are described. The vectors can include a nucleic acid encoding a membrane-bound anti-IL6 (mb-alL6) single chain variable fragment (scFv), and the transgenic T cells can express mb-alL6. The transgenic T cells are useful for suppressing proliferation of IL-6-dependent cells, reducing IL-6 concentration, or both. In one embodiment, the vector is a bicistronic construct encoding the mb-alL6 and an anti-CD19-41 BB-003 chimeric antigen receptor (CAR). In another embodiment, an anti-IL-6 scFv can be linked to a 41 BB and 003 domains to form an anti-IL-6 CAR. The transgenic T cells expressing said constructs can reduce the linker risk of cytokine release syndrome (CRS) in cancer patients being treated with CAR T cell or for the treatment of autoimmune diseases and inflammatory diseases in which cytokines are involved in pathogenesis.