The present invention pertains to methods for controlled ovarian hyperstimulation in a female subject using improved recombinant human follicle-stimulating hormone (rhFSH). The methods result in a high number of fertilizable oocytes even at low amounts of FSH administered to the female subject.

Provided are a method for preparing a mammalian ovum or embryo in which zona pellucida has been thinned or eliminated, and a method for fertilization using the mammalian ovum prepared by the aforementioned method. The resulting mammalian ovum or embryo is capable of realizing an improved fertilization rate and development rate when used for in vitro fertilization, transplantation of a fertilized ovum, or for preparation of an embryo in the early stages of development used in the production of a genetically modified animal.

The present invention relates to a process for in vitro spermatogenesis from male germinal tissue comprising conducting maturation of testicular tissue comprising germ cells in a bioreactor which is made of a biomaterial and comprises at least one cavity wherein the germinal tissue is placed, and recovering elongated spermatids and/or spermatozoa.

Disclosed are polar body genome restructured oocytes, the preparation method thereof and the use thereof in preparing the materials for preventing the occurrence of mitochondrial material genetic diseases.

The present invention relates to inorganic salts of nicotinamide mononucleotides and compositions of Formula I, useful in the treatment of disorders and diseases associated with deficiencies in NAD.sup.+:

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wherein A, M.sup.1, k, R.sup.1 and R.sup.2 are as described herein.

The present disclosure features systems and methods for isolating target entities (TEs) found in target entity-secondary entity complexes within a biological fluid by exploiting interactions between TE, secondary entities (SEs), and/or engineered surfaces (ESs). TE isolation involves a two-step process in which TE-SE complexes are initially captured using, e.g., size-based approaches or binding interactions between SEs and ESs, and then TEs are specifically released from the captured TE-SE complexes using, e.g., biochemical means, such as disassociating enzymes and/or binding inhibitors, or using physical properties of fluid flow, e.g., flow velocity and/or shear rate.

Haploid human embryonic stem cells and cell lines, haploid multipotent human cells, and haploid differentiated human cells are provided. In addition, methods of making and using the haploid human cells are provided.

Provided herein are methods and compositions for differentiating induced pluripotent stem cells into oogonia-like cells by overexpressing transcription factors such as ZNF281, LHX8, and/or SOHLH1.

Non-human animals and offspring thereof comprising at least one modified chromosomal sequence in a gene encoding a CD163 protein are provided. Animal cells that contain such modified chromosomal sequences are also provided. The animals and cells have increased resistance to pathogens, including porcine reproductive and respiratory syndrome virus (PRRSV). The animals and offspring have chromosomal modifications of a CD163 gene. The invention further relates to methods of breeding to create pathogen-resistant animals and populations of animals made using such methods.

A highly effective technique for fertilizing an oocyte with a sperm cell to produce a zygote can be carried out in a bioreactor and the zygote can optionally be cultured in the bioreactor into a blastocyst stage embryo for implantation into the uterus of a female recipient. The success rate and probability of fertilization is enhanced by operating the bioreactor reactor under conditions of inter-galactic motion which increases the probability of the sperm coming in close proximity to and fertilizing the oocyte. This method involves the steps of collecting an oocyte from a female donor, collecting sperm from a male donor, fertilizing the oocyte with the sperm in a rotating bioreactor to produce a zygote, culturing the zygote into a blastocyst stage embryo, and implanting the blastocyst stage embryo into a uterus of a recipient female.