Materials and methods for preparing a hydrogel are disclosed. Specifically, two separate solutions A and B, of which the solution A comprises enzyme horseradish peroxidase and the solution B comprises hydrogen peroxide is provided. At least one of solution A or B comprises calcium ions in the form of a pharmaceutically acceptable salt, and further the solution A and/or the solution B comprises hydroxyphenyl derivative of hyaluronan of a particular formula. A hydrogel based on hydroxyphenyl derivative of hyaluronan and a method of preparing and using the same is also disclosed.

The present invention relates to a method for preparing a porous scaffold for tissue engineering. It is another object of the present invention to provide a porous scaffold obtainable by the method as above described, and its use for tissue engineering, cell culture and cell delivery. The method of the invention comprises the steps consisting of: a) preparing an alkaline aqueous solution comprising an amount of at least one polysaccharide, an amount of a cross-linking agent and an amount of a porogen agent b) transforming the solution into a hydrogel by placing said solution at a temperature from about 4° C. to about 80° C. for a sufficient time to allow the cross-linking of said amount of polysaccharide and c) submerging said hydrogel into an aqueous solution d) washing the porous scaffold obtained at step c).

The present invention provides hyaluronic acid derivatives including one or more of each of repeating units represented by the formulae (Ia), (Ib), and (Ic) in which certain cationic sites, certain hydrophobic sites, and certain hydrophilic sites have been introduced. In addition, the present invention provides complexes of the hyaluronic acid derivatives with a drug and pharmaceutical compositions including the hyaluronic acid derivatives, in particular, complexes of the hyaluronic acid derivatives with a drug.

Compositions useful as dermal fillers and methods using such compositions to treat various skin and soft tissue conditions. The dermal fillers can comprise silk attached to hyaluronic acid using for example two cross linkers and can be used to treat of facial imperfections, facial defects, facial augmentations, breast imperfections, breast augmentations or breast reconstructions.

Physiological processes in plants are regulated and reinforced, and crop vitality, yield, quality and post-harvesting storage life are improved. A plant vitalizer containing an amino acid or its salt and an oligosaccharide is applied to plants.

Method of preparing a keratin-based biomaterial is provided. The method comprises a) reacting keratin with a polymer having at least one of an amine and carboxylic functional group in the presence of a carbodiimide cross-linking agent to form a cross-linked keratin-polymer material; and b) freeze drying the cross-linked keratin-polymer material to form the keratin-based bio-material. A keratin-based biomaterial thus prepared is also provided.

The present invention relates to a method for separating larvae into a pulp fraction and a liquid fraction, including the steps of introducing living larvae into a grinding apparatus whist adding water, grinding the larvae by means of counter-rotating screws and separating the ground biomass of larvae into a pulp and liquid fraction. In particular, the invention is applicable to the larvae of the black soldier fly and produces a chitin-rich pulp and a fat-and-protein-rich liquid fraction.

The present disclosure relates to synthesis of heparin, which may be bioequivalent to porcine USP Heparin Sodium. The synthesis may involve three intermediates starting from heparosan.

The present disclosure provides a facile method for selective isolation of hyaluronan (HA) from fluids and tissues by adsorption to a solid matrix. The method involves the use of specific solvent conditions and solid phase media favoring binding and subsequent elution of HA ranging in size from about 10 kDa to several MDa, while allowing separation of HA from other biological polyanions such as nucleic acids, sulfated glycosaminoglycans, chondroitin, and heparosan.

A process for treatment of biomasses including chitin with a process solvent selected from: an eutectic solvent consisting of a hydrogen bond acceptor and at least one hydrogen bond donor, an ionic liquid, and a mixture of the eutectic solvent and the ionic liquid, may include the steps of: A. mixing of a biomass with the process solvent and precipitation; and B. separating of the chitin precipitated in step A from a rest of the mixture. The hydrogen bond acceptor may be a choline salt with an C.sub.2-C.sub.6 organic acid, containing at least one carboxyl group and optionally substituted in the alkyl chain with at least one hydroxyl group. The at least one hydrogen bond donor may be an organic acid selected from: glycolic acid, diglycolic acid, levulinic acid, or imidazole. When the hydrogen bond acceptor is choline glycolate, the at least one hydrogen bond donor is not glycolic acid.