An object is to obtain a β-amylase having heat resistance even to high temperatures exceeding 60° C. By screening β-amylase producing bacteria from a soil sample, a novel Bacillus halosaccharovorans strain having a novel β-amylase with heat resistance and the β-amylase from the novel B. halosaccharovorans strain were obtained.

This specification discloses process for obtaining maltodextrin having DE between 17 and 19.9 and the maltodextrins obtained from the process. The disclosed maltodextrins can be provided as a powder or in shelf stable liquid form. The disclose maltodextrins have a polysaccharide profile similar to those observed for prior art maltodextrins, but make maltodextrin solutions having a high solids content, but reduced viscosity compared to prior art maltodextrins, on equivalent solids-in-solution basis. The process combines adds an alpha-amylase and a pullulanase enzyme to a polysaccharide mixture during a saccharification step. The disclosed maltodextrins make solutions at 50° C. and greater than 65% on a solids dry solids basis having a viscosity between 5,000 and 12,000 cP and having a water activity of less than 0.80.

The disclosure provides methods using mixed substrate cofeeding for bioproduct synthesis, which enables faster, more efficient, and higher yield carbon conversion in various organisms.

The present invention relates to a process for the production of one or more fermentation product from a sugar composition, comprising the following steps: a) fermentation of the sugar composition in the presence of a yeast belonging to the genera Saccharomyces, Kluyveromyces, Candida, Pichia, Schizosaccharomyces, Hansenula, Kloeckera, Schwanniomyces or Yarrowia: and b) recovery of the fermentation product,
wherein the yeast comprises the genes araA, araB and araD and the sugar composition comprises glucose, galactose and arabinose.

The disclosure relates, in general, to Glycogen Storage Disease and, in particular, to a method of treating Glycogen Storage Disease and to compounds and compositions suitable for use in such a method.

An isoamylase having improved heat resistance and an industrial method for producing maltose from starch.

The isoamylase is an isoamylase consisting of the amino acid sequence represented by SEQ ID NO: 1 or an isoamylase resulting from deletion, substitution, or insertion of one to several amino acid residues in the amino acid sequence represented by SEQ ID NO: 1, wherein at least valine at amino acid number 515 and methionine at amino acid number 570 are mutated to other amino acids.

The invention relates to processes for the conversion of biomass into carbohydrates, notable fermentable sugars. It provides means and methods for increasing the yield of enzymatic digestion of a biomass, in particular in those cases where cellulose is converted into sugars using a cellulose converting enzyme. More in particular, the invention relates to a method for producing a fermentable sugar from a lignocellulosic material wherein the lignocellulosic material is contacted with a laccase and an enzyme capable of degrading cellulose, either simultaneously or in a sequentially deferred fashion, wherein the laccase is the Bacillus spore coat protein CotA.

The present invention relates to variants of a parent alpha-amylase. The present invention also relates to polynucleotides encoding the variants and to nucleic acid constructs, vectors, and host cells comprising the polynucleotides, and methods of using the variant enzymes.

Provided are methods of treatment of cancers with combinations of and compositions containing a soluble hyaluronidase, such as a polymer-modified soluble hyaluronidase, and an immune checkpoint inhibitor for treating cancers, including solid and non-solid tumors. The combinations and compositions also are provided.

A process of recovering oil, comprising (a) converting a starch-containing material into dextrins with an alpha-amylase; (b) saccharifying the dextrins using a carbohydrate source generating enzyme to form a sugar; (c) fermenting the sugar in a fermentation medium into a fermentation product using a fermenting organism; (d) recovering the fermentation product to form a whole stillage; (e) separating the whole stillage into thin stillage and wet cake; (e′) optionally concentrating the thin stillage into syrup; (f) recovering oil from the thin stillage and/or optionally the syrup, wherein a protease and a phospholipase are present and/or added during steps (a) to (c). Use of a protease and a phospholipase for increasing oil recovery yields from thin stillage and/or syrup in a fermentation product production process.