Provided is an adipose tissue transport preservation solution. Specifically, the transport preservation solution includes: (a) 60-90 parts by weight of DMEM, high glucose and phenol red-free; (b) 10-30 parts by weight of DMEM/F-12, HEPES and phenol red-free; (c) 50-200 ng/mL of a bactericide; and (d) 2-10 mmol/L of L-glutamine. Adipose tissue can be transported and preserved by the preservation solution at a low temperature of 2-8 C. for 72 h or more.

Provided is an adipose tissue transport preservation solution. Specifically, the transport preservation solution includes: (a) 60-90 parts by weight of DMEM, high glucose and phenol red-free; (b) 10-30 parts by weight of DMEM/F-12, HEPES and phenol red-free; (c) 50-200 ng/mL of a bactericide; and (d) 2-10 mmol/L of L-glutamine. Adipose tissue can be transported and preserved by the preservation solution at a low temperature of 2-8 C. for 72 h or more.

Provided are a cold storage method and a cold storage liquid that are suitable for non-freezing refrigerated storage of human or animal stem cells such as iPS cells or embryos in a non-frozen state. According to an embodiment, the non-freezing refrigerated storage liquid is: a mixture liquid (HTM, HTMx2c13), in which MEM-alpha (Minimum Essential Medium Eagle (MEM) Alpha Modification) is mixed with a UW solution (UW_sln; BELZER UW (registered trademark) COLD STORAGE SOLUTION), or a modified UW solution (polymer component being changed to PVA), are mixed in a ratio of about 1/1 to 1/2; or a storage liquid having a composition equivalent to the mixture liquid, especially in respect of concentrations of potassium ions and sodium ions. And, arousal period(s) of maintaining a temperature of 30 C. to 37 C. is inserted at each of two to five days, into a non-freezing refrigerated storage period, which is made at 2 C. to 8 C. for 2 to 10 days or 4 to 20 days.

Provided are a cryopreservation liquid for bovine reproductive cells such as bovine sperms and a cryopreservation method thereof. Adopted is a cryopreservation liquid comprising: 0.3 to 0.9 w/w % of an amphoteric polyelectrolyte (an antifreeze polyamino acid), which is -poly-L-lysine wherein 50 to 99 mol % of amino groups of the -poly-L-lysine are blocked as carboxylated by having been reacted with the succinic anhydride; and glycerol, as dissolved in a physiological solution.

Provided are a cryopreservation liquid for bovine reproductive cells such as bovine sperms and a cryopreservation method thereof. Adopted is a cryopreservation liquid comprising: 0.3 to 0.9 w/w % of an amphoteric polyelectrolyte (an antifreeze polyamino acid), which is -poly-L-lysine wherein 50 to 99 mol % of amino groups of the -poly-L-lysine are blocked as carboxylated by having been reacted with the succinic anhydride; and glycerol, as dissolved in a physiological solution.

A composition for restoring or increasing tissue perfusion is provided. The composition includes polyethylene glycol polymers (PEG) with a molecular weight of 18,000-100,000 Da at a concentration of 5-20% by weight; PEG with a molecular weight of 1,000-10,000 Da at a concentration of 1-30% by weight; and water, wherein said PEG with a molecular weight of 18,000-100,000 Da and said PEG with a molecular weight of 1,000-10,000 Da are dissolved or dispersed in said water.

A method for storing or transporting cells, tissues or organs including: preparing a cytoprotectant formulation by mixing a first precursor solution including a balanced salt solution having a pH of from 7.6 to about 8.0 and a second precursor solution comprising water, D-glucose, glutathione, ascorbic acid, and optionally L-arginine at a pH of about 3-5; extracting cells, tissues or organs during a biopsy procedure; and storing or transporting the cells, tissues or organs in the cytoprotectant formulation. The cytoprotectant formulation can also be prepared by reconstituting a powder formulation in a saline or sterile water media to form a cytoprotectant formulation, where the powder formulation includes a reducing agent, an antioxidant, a sugar, a buffering agent, physiologically acceptable salts, optionally a nitric oxide substrate, and is reconstituted at a point of use in a saline or sterile water media.

A semen stabilization medium is described, which includes pH buffer agents, inorganic salts, organic compounds, and amino acids with antioxidant properties. The stabilization medium preserves semen viability for up to 72 hours when the semen is mixed with the stabilization medium and maintained at a temperature within a 20% tolerance of human body temperature.

This disclosure features tissue storage, irrigation, and infusion media compositions, and methods of using such compositions, for example, the temporary storage of cells and tissues prior to implantation or re-implantation, or for wound irrigation, wound packing, surgical site irrigation, or intravenous infusion. The tissue storage, irrigation, and infusion media compositions disclosed herein are useful in a healthcare or veterinary setting.

This disclosure features tissue storage, irrigation, and infusion media compositions, and methods of using such compositions, for example, the temporary storage of cells and tissues prior to implantation or re-implantation, or for wound irrigation, wound packing, surgical site irrigation, or intravenous infusion. The tissue storage, irrigation, and infusion media compositions disclosed herein are useful in a healthcare or veterinary setting.