A system and methodology for the preservation of red blood cells is described in which red blood cells are oxygen or oxygen and carbon dioxide depleted, treated and are stored in an anaerobic environment to optimize preparation for transfusion. More particularly, a system and method for extended storage of red blood cells from collection to transfusion that optimizes red blood cells prior to transfusion is described.

A system and methodology for the preservation of red blood cells is described in which red blood cells are oxygen or oxygen and carbon dioxide depleted, treated and are stored in an anaerobic environment to optimize preparation for transfusion. More particularly, a system and method for extended storage of red blood cells from collection to transfusion that optimizes red blood cells prior to transfusion is described.

Methods, additive kits and storage compositions for decreasing the deleterious effects of storage on pRBCs, for improving the aging process of stored pRBCs, and for preventing or ameliorating patient comorbidities following the transfusion or infusion of stored blood based on inhibiting acid sphingomyelinase during storage are provided.

Methods, additive kits and storage compositions for decreasing the deleterious effects of storage on pRBCs, for improving the aging process of stored pRBCs, and for preventing or ameliorating patient comorbidities following the transfusion or infusion of stored blood based on inhibiting acid sphingomyelinase during storage are provided.

An organ perfusion system (100) for extracorporeal perfusion of a heart (1) includes an organ chamber (15) having an aortic connector (17), and a first fluid flow path (19) comprising a pump interface (23) and an oxygenator (25), the aortic connector being fluidly connected to the first fluid flow path. The system is adapted for use in a method, wherein oxygenated perfusate is flowed into the heart via the aorta and desoxygenated perfusate is allowed to exit the heart via the inferior vena cava and/or the superior vena cava to thereby perfuse the heart in a substantially unloaded state. Such method may allow for improved recovery of the heart tissue during perfusion. The organ perfusion system may further comprise a perfusate reservoir (27) and/or a chassis, wherein the organ chamber is pivotable with respect to the chassis for holding the heart in a tilted position or to allow rotation of the organ chamber in a horizontal plane.

Described are systems, methods, and devices relating to normothermic extracorporeal support of an organ, tissue, or bioengineered graft comprising cross-circulation (XC) perfusion for prolonged periods (days to weeks) via an XC perfusion circuit in connection with an extracorporeal host (e.g., animal, patient, organ transplant recipient) are disclosed. The XC perfusion circuit comprises auto-regulation of blood flow based on the trans-organ blood pressure difference between arterial and venous pressure. Recipient support enabled 36 h of normothermic perfusion that maintained healthy lungs with no significant changes in physiologic parameters and allowed for the recovery of injured lungs. Extended support enabled multiscale therapeutic interventions in all extracorporeal lungs. Lungs exceeded transplantation criteria.

Described are systems, methods, and devices relating to normothermic extracorporeal support of an organ, tissue, or bioengineered graft comprising cross-circulation (XC) perfusion for prolonged periods (days to weeks) via an XC perfusion circuit in connection with an extracorporeal host (e.g., animal, patient, organ transplant recipient) are disclosed. The XC perfusion circuit comprises auto-regulation of blood flow based on the trans-organ blood pressure difference between arterial and venous pressure. Recipient support enabled 36 h of normothermic perfusion that maintained healthy lungs with no significant changes in physiologic parameters and allowed for the recovery of injured lungs. Extended support enabled multiscale therapeutic interventions in all extracorporeal lungs. Lungs exceeded transplantation criteria.

A face is maintained for transplant by circulating perfusate in through vascular vessels of a face after removal from a donor until transplant. The method includes placing a transplantable face on a support that maintains its preexisting structural shape within a chamber. Circulation is maintained by pumping a perfusate from a reservoir through vascular vessels, collecting perfusate from the chamber and returning it to the reservoir for recirculation. Prior to recirculating the collected perfusate it is filtered and irradiated with radiant energy thereby inactivating pathogens. Prior to return the collected perfusate is oxygenated and purified by dialysis with the dialysate being recharged by being passed through an ion exchange medium.

A face is maintained for transplant by circulating perfusate in through vascular vessels of a face after removal from a donor until transplant. The method includes placing a transplantable face on a support that maintains its preexisting structural shape within a chamber. Circulation is maintained by pumping a perfusate from a reservoir through vascular vessels, collecting perfusate from the chamber and returning it to the reservoir for recirculation. Prior to recirculating the collected perfusate it is filtered and irradiated with radiant energy thereby inactivating pathogens. Prior to return the collected perfusate is oxygenated and purified by dialysis with the dialysate being recharged by being passed through an ion exchange medium.

A packaging system includes a holder and an inner container. The holder includes a top portion and a bottom portion that cooperate to form a cavity configured to receive one or more medical products therein and to retain the one or more medical products therein. The inner container defines an interior region configured to receive the holder therein. The holder includes sidewalls that form the cavity, and the sidewalls include channels configured to fluidly connect the cavity to the interior region of the inner container.