Provided is an organ preservation solution for use in transplantation, which is capable of effectively preventing the occurrence of ischemia-reperfusion injury. Prepared is an organ preservation solution comprising a mixture of the following components: (a) potassium chloride, disodium hydrogen phosphate, sodium dihydrogen phosphate, L-ascorbic acid, L-ascorbic acid phosphate ester, and D-glucose; (b) potassium chloride, disodium hydrogen phosphate, sodium dihydrogen phosphate, L-ascorbic acid, L-ascorbic acid phosphate ester, glycine, and D-glucose; (c) potassium chloride, disodium hydrogen phosphate, sodium dihydrogen phosphate, L-ascorbic acid, L-ascorbic acid phosphate ester, L-cysteine, glycine, and D-glucose; or (d) potassium chloride, disodium hydrogen phosphate, sodium dihydrogen phosphate, L-ascorbic acid, L-ascorbic acid phosphate ester, L-cysteine, glycine, an iron compound, and D-glucose.

The invention provides cryopreserved compositions of cells, wherein the compositions are advantageously in the form of self-sustaining bodies that can be individually handled and combined independently of a container, allowing for easy customization of the eventual pooled preparation. The invention also provides pre-pooled stacks of the self-sustaining cryopreserved compositions for eventual thawing to produce pooled preparations of cells, and methods of creating the same.

An organ perfusion apparatus and method monitor, sustain and/or restore viability of organs and preserve organs for storage and/or transport. Other apparatus include an organ transporter, an organ cassette and an organ diagnostic device. The method includes perfusing the organ at hypothermic and/or normothermic temperatures. The methods further include perfusing the organ at hypothermic and/or normothermic temperatures with a second perfusate containing a substance for reacting with the organ. Condition of the organ may be automatically monitored, and the perfusion process can be automatically controlled using a control program.

The straw comprises a tube (11) extending between a first end and a second end and comprises a gas-permeable, liquid-tight plug, said plug being arranged in the tube (11) in the vicinity of the first end of same and extending between a first end turned towards the first end of the tube (11) and a second end turned towards the second end of the tube (11). Said plug comprises an element (14) impregnated with multivalent cations (22) at least in the vicinity of a first end turned towards the second end of the tube (11). The system comprises the straw and a liquid medium provided to produce, by mixing, in predefined conditions, the liquid substance (21). The medium contains an alginate (23) in solution.

The system comprises a straw and a liquid dilution medium. The straw comprises a tube (11) extending between a first end and a second end and comprises a gas-permeable, liquid-tight plug, said plug being arranged in the tube (11) in the vicinity of the first end of same and extending between a first end turned towards the first end of the tube (11) and a second end turned towards the second end of the tube (11). Said plug comprises an element (14) impregnated with a reagent (22) at least in the vicinity of a first end turned towards the second end of the tube (11). The liquid medium is provided to produce, by mixing, in predefined conditions, the liquid substance (21). The reagent (22) and the medium(27) are configured such that, when the substance (21) comes into contact with the first end of the impregnated element (14), it forms a hydrogel.

Compositions, methods, systems/devices and media are provided for maintaining a harvested organ in a functioning and viable state prior to implantation. The organ perfusion apparatus includes a preservation chamber for storing the organ during the preservation period. A perfusion circuit is provided having a first line for providing an oxygenated fluid to the organ, and a second line for carrying depleted fluid away from the organ. The perfusion apparatus also includes a device operably associated with the perfusion circuit for maintaining the organ at a substantially normothermic temperature.

Compositions, methods, systems/devices and media are provided for maintaining a harvested organ in a functioning and viable state prior to implantation. The organ perfusion apparatus includes a preservation chamber for storing the organ during the preservation period. A perfusion circuit is provided having a first line for providing an oxygenated fluid to the organ, and a second line for carrying depleted fluid away from the organ. The perfusion apparatus also includes a device operably associated with the perfusion circuit for maintaining the organ at a substantially normothermic temperature.

The present invention relates to a method for freezing and/or freeze-drying mitochondria isolated from cells, and a use of frozen and/or freeze-dried mitochondria. The frozen mitochondria according to the present invention showed similarity to non-frozen mitochondria in terms of anti-inflammatory activity and activity of energy metabolism-related enzymes. Also, the freeze-dried mitochondria showed similar levels of anti-inflammatory activity, activity of energy metabolism-related enzymes, and long-term preservation efficacy after recovery to those in the mitochondria before freeze-drying. Therefore, allowing isolated mitochondria to find a wide spectrum of applications in the treatment of various diseases caused by mitochondrial dysfunction or impairment, the frozen and/or freeze-dried mitochondria according to the present invention is expected to greatly enhance the commercial availability of therapeutics containing mitochondria.

A method for controlling at least one pump in a perfusion apparatus for delivering a fluid to at least one organ through a plurality of vessels for maintaining the viability of the at least one organ, the method including supplying a fluid to a first vessel of an organ and to a second vessel of an organ; measuring a first parameter of the fluid flowing in the first vessel and a second parameter of the fluid flowing in the second vessel; and executing direct control of the second parameter of the fluid flowing in the second vessel to influence the first parameter of the fluid flowing in the first vessel.

Solutions and suspensions comprising polymerized hemoglobin derived from human blood are disclosed. The solutions and suspensions may comprise cell culture medium, an enzyme (such as a protease), and/or a buffer. Processes of preparing the solutions and suspensions are also disclosed. The solutions and suspensions may be employed in methods of isolating mammalian cells, such as pancreatic islets, methods of preserving mammalian tissue and organs, methods of aiding the recovery of mammalian cells following their isolation, methods of maintaining mammalian cells, methods of propagating mammalian cells, and methods of treating a mammal with diabetes.