Disclosed are devices and methods for non-cryogenic vitrification of biological materials that include the steps of providing one or more capillary channels of which a first opening is operably in contact with a moisture containing vitrification mixture made of a biological material and a vitrification agent. The capillary absorbs and transports the moisture to the second opening through capillary action, and the moisture is subsequently evaporated into a surrounding low humidity atmosphere until the vitrification mixture enters into a vitrified state.

The carriers that are available in the market don't maintain all the necessary parameters that I am mentioning in my invention. Many of them are: 1) Costly 2) Allow the transport of cells for short period of time 3) Are suitable for transporting certain type of cells 4) Don't include control for all the parameters that are required for the experiments 4) Need extra steps which can affect the experiment/organs and waste the time, efforts and money of the researchers. 5) Limit the researchers for using containers with certain sizes and materials.

Alternatively, the Mini-incubator carrier box in my invention is an economic transport system. It enables the researchers to fix different containers using clamps and screws and to transport the organs/samples/cells in any container for long period of time and long distance under optimized conditions without the need of extra steps/costs.

An automated system and method of preparing oocytes, embryos, or blastocysts for cryopreservation. The method entails delivering two or more solutions into a container holding oocytes, embryos, or blastocysts, and controlling the flow of the solutions to gradually change the concentration of cryoprotectants and dehydrating agents in the container to minimize shock to the oocytes, embryos or blastocysts.

The present invention, in some embodiments thereof, relates to a method and scalable devices for hyperfast cooling and re-warming of samples. More specifically, it relates to cryogenic preservation of biological samples via vitrification. It includes: a liquid sample placed at ambient temperature in a flat thermo conductive container that in some embodiments additionally contains a detachable disposable or sterilizable thermo conductive spiral; transferring the sample to a cooling chamber using a linear percussion stepping motor drive; hyperfast cooling of the sample using streams of pressurized liquid coolant; transferring the sample to a detachable shipping/storage chamber filled with liquid coolant, from which the sample can be transferred to another vessel that contains liquid cryogenic coolant and moved back to the shipping/storage chamber. This chamber can be then attached to a re-warming chamber, in which the sample is heated to a biologically tolerant temperature above 0 degrees Celsius in a hyperfast manner.

A method for freezing a plurality of conditioning tubes is provided, each tube filled with a predetermined volume of biological substance, including arranging each conditioning tube directly into a conditioning unit placed in a cooling enclosure then causing the enclosure to be passed through by a flow of cooling agent and simultaneously driving the conditioning unit (6) in rotation, and providing the unit (6) with a greater capacity than the plurality of tubes; wherein the step of arranging each tube directly in a unit is carried out by placing the plurality of tubes into the unit, and the step of driving the unit in rotation is implemented by setting each tube in motion with respect to the unit and with respect to the other tubes.

An active cooler having an insulated five sided box having an inner chamber, the box has an openable lid hingedly attached to the upper edge of the box, a temperature sensor in the inner chamber for detecting temperature readings of the inner chamber when the lid is closed, a peltier cooling element operably engaged to the inner chamber to remove heat, a voltage regulator operably connected to the sensor to change the cooling element in response to temperature readings from the sensor, and a wireless transmitter connected to the temperature sensor for transmission of temperature readings and detection of pre-determined temperatures. The cooler may also have a program that is operated by an onboard computer to generate temperature and time related data, transmit the data via cell phone, internet, or local area and interact with hand held devices via specialized application software.

A method and apparatus for the processing of tissue and cellular material during cryopreservation and/or processing for microscopy. The method and apparatus maximizes heat transfer coefficients by using liquid-free cryopreservation protocols and maximizing glass transition characteristics through increasing pressure during cryopreservation. Cooling rates combined with megapascal pressures reduced the required concentration of cryoprotective agents (CPAs) needed for ice-free cell and tissue cryopreservation.

An automated, ultra-low temperature freezer having multiple structural features that reduce heat transfer into the freezer, protect its internal mechanical devices against low temperature mechanical binding of their movements, allow defrosting and autoclaving as a result of only minimal changes to the conventional CO.sub.2 emergency backup system. A group of freezers are arranged so they can simultaneously provide an HVAC function. A vial management system allows biological samples or vials to be automatically placed in and recovered from the freezer and associates the temperature history with each sample or vial that it was subjected to during its storage.

An automated, ultra-low temperature freezer having multiple structural features that reduce heat transfer into the freezer, protect its internal mechanical devices against low temperature mechanical binding of their movements, allow defrosting and autoclaving as a result of only minimal changes to the conventional CO.sub.2 emergency backup system. A group of freezers are arranged so they can simultaneously provide an HVAC function. A vial management system allows biological samples or vials to be automatically placed in and recovered from the freezer and associates the temperature history with each sample or vial that it was subjected to during its storage.

An automated, ultra-low temperature freezer having multiple structural features that reduce heat transfer into the freezer, protect its internal mechanical devices against low temperature mechanical binding of their movements, allow defrosting and autoclaving as a result of only minimal changes to the conventional CO.sub.2 emergency backup system. A group of freezers are arranged so they can simultaneously provide an HVAC function. A vial management system allows biological samples or vials to be automatically placed in and recovered from the freezer and associates the temperature history with each sample or vial that it was subjected to during its storage.