Systems and methods for perfusing the target tissue are provided. The system can include a processor and a bioreactor. The bioreactor includes a media reservoir for storing media, a peristaltic pump for transferring the media, a perfusion chamber for culturing the target tissue with the media, and a plurality of sensors. The perfusion chamber, the peristaltic pump, and the media reservoir are coupled through the tubing. The processor includes an artificial intelligence (AI) module and is configured to generate periodic reports on the metabolic rate of the target tissue through the plurality of sensors and control an operation of the bioreactor for extending a perfusion period.

Systems and methods for perfusing the target tissue are provided. The system can include a processor and a bioreactor. The bioreactor includes a media reservoir for storing media, a peristaltic pump for transferring the media, a perfusion chamber for culturing the target tissue with the media, and a plurality of sensors. The perfusion chamber, the peristaltic pump, and the media reservoir are coupled through the tubing. The processor includes an artificial intelligence (AI) module and is configured to generate periodic reports on the metabolic rate of the target tissue through the plurality of sensors and control an operation of the bioreactor for extending a perfusion period.

Methods and compositions for the prevention or reduction of platelet transfusion associated complications are provided. The subject methods include modifying donor whole blood or platelets prior to transfusion to prevent or reduce alloimmune platelet refractoriness.

Methods and compositions for the prevention or reduction of platelet transfusion associated complications are provided. The subject methods include modifying donor whole blood or platelets prior to transfusion to prevent or reduce alloimmune platelet refractoriness.

A method and a system for a normothermic machine perfusion of an ex vivo liver is provided. The method comprises the following steps: (a) generating a flow of a perfusate through an ex vivo liver, (b) stimulating a bile secretion of the ex vivo liver, and (c) determining a volume of bile secreted by the ex vivo liver, and subsequently adding an oxygen carrier, e.g., whole blood to the perfusate, wherein the added oxygen carrier has a volume that equals at least 20% of the determined volume of secreted bile.

A method and a system for a normothermic machine perfusion of an ex vivo liver is provided. The method comprises the following steps: (a) generating a flow of a perfusate through an ex vivo liver, (b) stimulating a bile secretion of the ex vivo liver, and (c) determining a volume of bile secreted by the ex vivo liver, and subsequently adding an oxygen carrier, e.g., whole blood to the perfusate, wherein the added oxygen carrier has a volume that equals at least 20% of the determined volume of secreted bile.

A fusion protein is provided which comprises a first and a second peptide. The first peptide enables the fusion protein to bind to a receptor expressed on a cell, and the second peptide having a cleavage site that enables the fusion protein to kill said cell. The fusion protein is thus useful for the prevention or treatment of an infection caused by a pathogen. Nucleic acids encoding the fusion protein and methods of making and using the fusion protein are also provided.

A fusion protein is provided which comprises a first and a second peptide. The first peptide enables the fusion protein to bind to a receptor expressed on a cell, and the second peptide having a cleavage site that enables the fusion protein to kill said cell. The fusion protein is thus useful for the prevention or treatment of an infection caused by a pathogen. Nucleic acids encoding the fusion protein and methods of making and using the fusion protein are also provided.