Current methods for detection of microbial contaminants on surfaces use swabbing/wiping to extract microbes for analysis. This removes easily transferable microbes but fails to extract microbes living in biofilms, which reduces sensitivity and may mask the true degree of contamination. The current disclosure provides an enzyme cocktail that disrupts the biofilm and improves the extraction of live microbes for analysis. Applicant's enzyme system is particularly useful for the application to a variety of surfaces, but particularly on a variety of food processing surfaces. Utilization of Applicant's enzyme cocktail makes possible the extraction of a representative sample of live microorganisms present on a surface, including film forming microorganisms, without affecting non-film forming microorganisms also present on a surface.

The present invention relates to polypeptide variants of porcine trypsin, to nucleic acid molecules encoding these variants, and to host cells comprising such nucleic acid molecules. It also relates to the use of these variants in methods for producing insulin. The invention further relates to the use of these variants as medicaments, as food ingredients, or as feed ingredients and to the use of these variants within a process of manufacturing a food ingredient or a feed ingredient.

The present invention relates to the novel trypsin ZT isoforms. In particular, the invention relates to the use of trypsin ZT isoforms in medical devices, pharmaceuticals and cosmetics.

A method for purifying activated human MMP in E. coli without the use of urea or APMA is provided. In the method, a non-ionic detergent is used in a lysis buffer to solubilize MMP, and the protease activities of trypsin and MMP are utilized to digest the E. coli proteins and activate pro-MMP1.

Various aspects described herein relate to electrochemical devices, e.g., for separation of one or more biomolecules from a solution, and methods of using the same. Methods for using the electrochemical devices for biocatalysis are also described herein.

Provided herein are methods, reagents, and kits for isolating polypeptides, such as a proteome. Also provided herein is a modified trypsin polypeptide that is resistant to autolysis, and that can be selectively-separated from a biological sample once digestion is complete.

The present invention discloses monodisperse protein-dendron conjugates that self-assemble to generation-dependent supramolecular protein assemblies of different size and surface charges. The invention further provides a process for synthesis of protein-dendron conjugates containing hydrophobic dendron of different generations.

Pandemic COVID 19 causes global crisis in human health and economy. The pathogen of COVID 19, like the Influenzas causing common flus, belongs to a common type of virus called Ribonucleic Acid (RNA) virus. RNA viruses are composed of three structural components: genomic RNA, envelop and nuclear proteins, and bilayer lipid membrane. Targeting COVID 19 and all RNA viruses, the present invention describes an effective disinfectant system, which applies the combination of RNases, proteases, and detergents, at a broad range of concentration, pH, and temperature in the utilization, to eliminate the RNA viruses from any contaminated surfaces, airways, and filters. The disinfectant system provides an effective, convenient, environment friendly, and safe solution, for commercial and customer use, to disinfect RNA virus pathogens in concern.

Disclosed are compositions and formulations comprising enzymes or other biocatalyst that cleave surface-accessible DNA polymers and/or glycoprotein carbohydrate chains at galactose residues in dental calculus, and optionally further include one or more proteolytic enzymes, thereby destroying the structural integrity of the calculus, and allowing it to be readily removed without requiring special treatment by a trained dental professional. Also disclosed are methods for removing dental calculus using the disclosed compositions and formulations.

Variants of chymosin with improved milk-clotting properties are disclosed.