A method for producing -Glu-Val-Gly is described, wherein the method includes the steps of cultivating a -Glu-Val-Gly-producing bacterium belonging to the family Enterobacteriaceae in a culture medium so that the -Glu-Val-Gly accumulates in the culture medium or the cells of the bacterium, or both, and collecting the -Glu-Val-Gly from the culture medium or the cells of the bacterium, or both. The bacterium has been modified to overexpress a gene encoding a protein having L-threonine 3-dehydrogenase activity and a gene encoding a protein having 2-amino-3-oxobutanoate coenzyme A ligase activity.