A dispersing device includes: a casing having a liquid inlet; a rotating body accommodated in the casing and pivotably attached to a rotating shaft from one end of the rotating body; a liquid channel having, on the other end of the rotating body, a passage through which the liquid from the liquid inlet passes, and , inside the rotating body, a segment extended radially around the rotating shaft toward an outer side perpendicular to the rotating shaft and from the other end of the rotating body toward the one end of the rotating body in a direction of the rotating shaft axis and in which a cross section shape perpendicular to the rotating shaft is annular; and one connecting hole in the rotating body connecting the liquid channel with the exterior of the rotating body downstream of the liquid channel.

Apparatus for controlling flow of fluid in a fluid body containing a first fluid, the apparatus including: a baffle at least partially submerged in the fluid body, the baffle including: in inlet that in use is coupled to a fluid supply adapted to supply a second fluid; at least one outlet; at least one internal channel extending from the inlet to the at least one outlet to thereby allow second fluid to be expelled from the at least one outlet; and, an outer surface which acts with the at least one outlet to at least partially guide second fluid expelled from the outlet to induce flow within the fluid body and thereby agitate the fluid body.

A jet-mixer for a fluid mixing system includes a housing including a mixer port formed in an inner surface of the housing, and a rotor rotatably disposed in the housing, the rotor including a rotor blade, wherein the rotor includes a first angular position in the housing restricting fluid flow through the mixer port, and a second angular position allowing fluid flow through the mixer port, wherein, in response to rotation of the rotor in the housing, the jet-mixer is configured to inject a first fluid jet through the mixer port into the passage of the fluid conduit that has a pulsed velocity profile.

According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.

Methods and systems for chromatography are disclosed that employ a flexible container configured to fit within a support structure and adapted to receive a filtration or absorptive medium, such as a chromatography resin. The flexible container can include at least one inlet, at least one outlet, and a separation barrier peripherally sealed within the container to separate the container into a resin containing portion and a drainage portion. The barrier can be configured to exclude the resin material from the drainage portion during use while allowing fluids to pass therethrough. The disposable chromatography system can further include one or more agitators disposed within the flexible container and adjustably configured to be raised or lowered in the flexible container. When the agitator is in the raised position, the resin packing material can operate in a settled, packed-bed configuration. Alternatively, the agitator in the lowered position permits the chromatography resin packing material to operate in a mixed, slurry configuration.

A method of mixing a pharmaceutical solution including adding a gas into an interior compartment of a mix bag to form a headspace. The interior compartment of the mix bag includes a top portion and a bottom portion. The headspace adjacent to the top portion contains gas. The method includes adding a solvent into the mix bag, and establishing a bubble column in the interior compartment by activating a recirculation assembly. The recirculation assembly includes a connecting pathway operably coupled to a recirculation pump. A first end of the connecting pathway is coupled to a top gas recirculation port and a second end is coupled to a bottom gas recirculation port of the mix bag such that the recirculation pump draws the gas from the headspace and delivers the gas to the interior compartment via the bottom gas recirculation port. The method includes adding a solute into the mix bag.

A method and a device for the continuous purification of a domestic or industrial water stream. After passing through a biological reactor to obtain a sludge of between 4 and 12 g/l of Dry Solids, the method involves feeding a series of chambers separated by restrictions at a flow rate q, injecting air into a second chamber at a flow rate Q>q, in order to obtain an emulsion, injecting a flocculant into a third chamber, and recovering the degassed emulsion in a drain pan. Flocculated and aerated sludge floats on top, such that the remaining liquid centrate has a Dry Solids content less than 100 mg/l and has a positive redox potential >50 mV and a value greater than 100 mV relative to the redox potential of the sludge at the inlet of the chambers, and is reinjected upstream from or into the biological reactor.

A mixing apparatus for mixing powder and liquid, includes: an apparatus main body having an internal space; a cylindrical member arranged in the internal space with one end facing upward and the other end facing downward, the one end and the other end being opened; a powder dispersing portion arranged above the apparatus main body, the powder dispersing portion dispersing the powder into a space of the cylindrical member; a liquid spraying portion arranged in a vicinity of the powder dispersing portion, the liquid spraying portion spraying the liquid into a space of the cylindrical member; a collection portion arranged below the apparatus main body, the collection portion collecting a mixed powder consisting of the powder dispersed by the powder dispersing portion and the liquid sprayed by the liquid spraying portion; a filter arranged around the cylindrical member in the internal space; and a discharge port discharging air in the internal space through the filter.

Various embodiments of a low-volume sequencing system are provided herein. The system can include a low-volume flowcell having at least one reaction chamber of a defined volume (e.g., less than about 100 l). The system can also include an automated reagent delivery mechanism configured to reversibly couple with the inlet port corresponding to a target reaction chamber thereby placing allowing for reagent to be accurately moved from a storage container to the reaction chamber with minimal reagent waste. The flowcells can include a plurality of reaction chambers (e.g., 6) thereby allowing for parallel analysis of multiple samples. Various methods of analyzing a biomolecule are also provided herein.

According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.