A system comprises a fluid supply, a pressure regulator, a pressure valve, and a vent assembly. The fluid supply stores a volume of a fluid and is fluidly coupled to an outlet tube. The outlet tube is configured to be fluidly coupled to at least a portion of an organ environment. The organ environment configured to hold an organ. The pressure regulator is fluidly coupled to the outlet tube and is configured to maintain a fluid pressure of the fluid being supplied to the organ environment from the outlet tube. The fluid being supplied is at a pressure range above ambient to maintain the organ in an inflated position. The pressure valve is fluidly coupled to the outlet tube and inhibits increase of the fluid pressure above a threshold. The vent assembly removes excess fluid from the organ environment and includes a vent tube disposed within the organ environment and a vent valve disposed outside of the organ environment.

A system comprises a fluid supply, a pressure regulator, a pressure valve, and a vent assembly. The fluid supply stores a volume of a fluid and is fluidly coupled to an outlet tube. The outlet tube is configured to be fluidly coupled to at least a portion of an organ environment. The organ environment configured to hold an organ. The pressure regulator is fluidly coupled to the outlet tube and is configured to maintain a fluid pressure of the fluid being supplied to the organ environment from the outlet tube. The fluid being supplied is at a pressure range above ambient to maintain the organ in an inflated position. The pressure valve is fluidly coupled to the outlet tube and inhibits increase of the fluid pressure above a threshold. The vent assembly removes excess fluid from the organ environment and includes a vent tube disposed within the organ environment and a vent valve disposed outside of the organ environment.

A system and method for maintaining an oxygen concentration of a biological sample. The oxygen concentration can be maintained by measuring the oxygen concentration within the biological sample and adjusting a rate of an oxygen supplier in response to this measurement. For example, when the oxygen concentration is below a threshold, oxygen can be delivered to the biological sample at a higher rate.

A system and method for maintaining an oxygen concentration of a biological sample. The oxygen concentration can be maintained by measuring the oxygen concentration within the biological sample and adjusting a rate of an oxygen supplier in response to this measurement. For example, when the oxygen concentration is below a threshold, oxygen can be delivered to the biological sample at a higher rate.

An apparatus for perfusing a lung includes: an enclosure configured to provide physical protection to a lung; and a fluid flow loop extending from an outlet connection of the enclosure to an inlet connection of the enclosure, the fluid flow loop including a pump, an oxygenator, and a waveform generator; at least one sensor operable to sense a parameter of a perfusate flowing within the fluid flow loop and generate a signal representative thereof, a negative pressure ventilation apparatus configured to force respiration of the lung through cyclic changes of gas pressure between the lung and the enclosure; and a control and monitoring unit configured to receive the signal from the at least one sensor and adjust the parameter in response thereto.

An apparatus for perfusing a lung includes: an enclosure configured to provide physical protection to a lung; and a fluid flow loop extending from an outlet connection of the enclosure to an inlet connection of the enclosure, the fluid flow loop including a pump, an oxygenator, and a waveform generator; at least one sensor operable to sense a parameter of a perfusate flowing within the fluid flow loop and generate a signal representative thereof, a negative pressure ventilation apparatus configured to force respiration of the lung through cyclic changes of gas pressure between the lung and the enclosure; and a control and monitoring unit configured to receive the signal from the at least one sensor and adjust the parameter in response thereto.