The systems and methods relate to cyclically inflating and deflating a lung. The lung can be stored and transported while pumping a gas into the lung and removing another gas from the lung. The cyclical inflation and deflation of the lung can be performed at a rate enhanced or optimized for lung performance.

A Descemet Membrane Endothelial Keratoplasty (DMEK) holder for placement within a vial for supporting a corneal tissue inserter is provided. The DMEK holder includes a top and an opposite bottom, at least one support member extending between the top and the bottom, with a bottom portion of the at least one support member sized and shaped to fit against a bottom inner surface of the vial to thereby support the corneal tissue inserter within the vial, and a first annular member having an opening sized and shaped such that a bottom end of the corneal tissue inserter is insertable through the opening and the first annular member separates the corneal tissue inserter from an inner surface of the vial and positions the corneal tissue inserter within the vial away from inner surfaces of the vial to thereby improve retrieval of the corneal tissue inserter by a surgeon.

A holding tool is configured to be used together with a container and a lid for the container to sandwich the container and the lid. The holding tool includes a base on which the container is placed, and a cover configured to cover the lid. The base and the cover each have an opening at a central portion and are configured to engage with each other to press and sandwich the container and the lid upward and downward.

An article including, either: a frame having an interior cavity, and a flexible liner situated within the interior cavity; or a deformable frame having an interior cavity, as defined herein.
Either frame can receive a sample, such as liquid having suspended live cells, and cooling freezes the sample to a solid sample. The solid sample can be readily ejected from the article having either a frame with the flexible liner, or the deformable frame, without extensive thawing. The solid sample can be rapidly displaced from the article by the methods and apparatus disclosed herein.

The carriers that are available in the market don't maintain all the necessary parameters that I am mentioning in my invention. Many of them are: 1) Costly 2) Allow the transport of cells for short period of time 3) Are suitable for transporting certain type of cells 4) Don't include control for all the parameters that are required for the experiments 4) Need extra steps which can affect the experiment/organs and waste the time, efforts and money of the researchers. 5) Limit the researchers for using containers with certain sizes and materials.

Alternatively, the Mini-incubator carrier box in my invention is an economic transport system. It enables the researchers to fix different containers using clamps and screws and to transport the organs/samples/cells in any container for long period of time and long distance under optimized conditions without the need of extra steps/costs.

A device and method for preserving organisms (e.g. causative organisms in a case of osteomyelitis) in a biopsy sample during transport or storage in a manner which obviates or mitigates deleterious effects of lengthy periods of transport or storage on the success rate of determining the identity of the organism and subsequent testing. The device includes a reservoir which contains a transport medium, an outlet for releasable connection with a biopsy sample housing (e.g. a syringe with a luer connector element) containing a biopsy sample, and a closure member to seal the outlet before use. The transport medium can be delivered from the reservoir to the syringe, when the two are connected, and the reservoir-syringe unit can be transported as a sealed unit.

A medical carbon monoxide generator provides for a solid carbon material that may be heated at substantially normal atmospheric pressure to provide a source of medical quality carbon monoxide. The heating source may be an electrical filament or laser controllable by a microcontroller to provide accurate delivery rates and amounts. In one embodiment, a replaceable cartridge holding the carbon material may be used.

An apparatus intended for evaluation, preservation and perfusion of an organ, such as a lung. The apparatus includes a container with a bottom portion, an insert portion and a lid portion. A pulmonary artery tube is intended to be connected to the lung pulmonary artery and a trachea tube is intended to be connected to the trachea of the lungs and bent tube connects the pulmonary artery tube to a circuit for providing a fluid to the pulmonary artery is provided. The circuit includes a pump, an oxygenator, an optional leukocyte-filter, and a holder for connecting the trachea tube to a source of respiration. There is an oxygenator tube set and a leukocyte-filter tube set. A supporting structure for the container comprises a recess sized for enclosing said container and a display panel. Moreover, there are two handles, which may be unfolded into a position for supporting a sterile cloth.

The present disclosure provides for the use of a temperature controlled shipping container as a method for the packaging and distribution of mammalian cells, e.g., in induced pluripotent stems cells (iPSc), and various organ cells differentiated from the iPSc. In one embodiment, a shipping box that utilizes a vacuum, desiccant and insulation construct is employed to package iPSc and their derivatives or progeny that are or have been deposited onto microwell plates or tubes that contain a nutrient solution during storage and shipping. The present system and method can maintain the cells in the containment vessels at or below 10 C. for at least 24 hours and in one embodiment up to 96 hours.

Described are flexible vessels that are useful for containing a liquid and freezing the liquid, and a flexible resistive heating element that is adapted to heat the frozen liquid to cause the frozen liquid to melt.