An allogeneic dendritic cell (alloDC) tumor vaccine, and a preparation method and use thereof are provided, belonging to the technical field of tumor cell vaccines. An allogeneic chimeric antigen receptor (CAR) dendritic cell (DC) tumor vaccine is an alloDC that recombinantly expresses a CAR and a tumor vaccine. The alloDC serves as a basic cell, where an allogeneic cell for cell therapy shows characteristics of expanding cell sources, improving cell quality, and shortening cell preparation time. Meanwhile, effectiveness and safety of the allogeneic CAR-DC tumor vaccine are verified for tumor treatment. The development of such a novel vaccine overcomes a long-standing limitation of the cell therapy relying on autologous cells, and is conducive to expanding therapeutic application and scale of the CAR-DC and improving a quality of cell preparation, thereby providing new strategy and application for conquering solid tumors.

Disclosed are a single-chain fragment variable (scFv) targeting KRAS G12V, a chimeric antigen receptor (CAR), and a use thereof. Based on the KRAS G12V target, a T cell receptor (TCR) is modified. An extracellular signaling domain of the TCR for recognizing a tumor-specific antigen (TSA) is retained and linked in series to an extracellular spacer, a transmembrane domain, and a CD3-derived intracellular signaling domain in the conventional CAR structure, such that the modified CAR can specifically recognize a KRAS G12V mutant polypeptide presented by HLA-A*02:01. Moreover, based on the advantages of CAR-T cell/NK cell therapy, potential new tumor treatment options are explored to lay a foundation for clinical trials.

Provided is a T cell receptor (TCR) capable of specifically binding to KRAS G12V mutant antigens; the mutant antigen short peptide VVGAVGVGK is capable of forming a complex with HLA A1101, and the TCR specifically binds to said complex. Also provided in the present invention are a nucleic acid molecule encoding the TCR and a vector comprising the nucleic acid molecule. Also provided are TCR-transduced cells.