The invention relates to the field of cell differentiation and cryopreservation, in particular of pancreatic lineage cells. It provides methods for differentiating cells of the pancreatic lineage, in particular to islet-like clusters. It further provides methods for freezing cells of the pancreatic lineage, in particular endocrine progenitor cells. It also provides new pancreatic lineage cell populations.

According to one embodiment, a cell detachment method comprising: a detachment step of discharging a liquid used for cryopreservation of cells toward a surface of a culture container in contact with the cells, where the cells are cultured, thereby detaching the cells from the surface; and a recovery step of recovering the detached cells together with the discharged liquid used for cryopreservation.

Described herein are methods, compositions, and kits for isolation of mitochondria from cryopreserved primary cells (e.g., fibroblast cells, mesenchymal stromal/stem cells) to be used in mitochondrial organelle transplantation (MOT) methods and compositions.

The present application provides formulations of enhanced antigen presenting cells (enhanced APCs), wherein the formulation comprises: the enhanced APCs comprising an antigen (e.g., a human papillomavirus (HPV) antigen) and one or more of the following: cryopreservation medium, hypothermic preservation medium, and human serum albumin. Also provided herein are methods of producing such formulations and the enhanced APCs.

An object of the present invention is to provide an agent for promoting undifferentiation that dissolves a drug, as DMSO or the like does, but is capable of promoting undifferentiation without inducing cell differentiation when added to a medium. The agent for promoting undifferentiation of the present invention has an aprotic zwitterion represented by the following formula (1) ##STR00001##
wherein A is an anion selected from the group consisting of SO.sub.3.sup., COO.sup., OPO(H)O.sup., OPO(CH.sub.3)O.sup. and OPO(OR.sub.3)O.sup., R.sub.1 is an alkyl group having 1 to 8 carbon atoms and optionally containing one or two oxygen atoms in the molecular chain, R.sub.2 is an alkylene group having 3 to 5 carbon atoms, and R.sub.3 is hydrogen or an alkyl group optionally having a heteroatom in the molecular chain.

The disclosure relates to composition and method for cryopreservation of mitochondria, cryopreserved composition comprising mitochondria and their therapeutic use.

Disclosed herein is a method for producing mature dendritic cells from peripheral blood mononuclear cells (PBMCs). The method includes the steps of, treating the PBMCs with a cultivating medium supplemented with interleukin 4 (IL-4) and granulocyte-macrophage colony-stimulating factor (GM-CSF) to produce immature dendritic cells; and then treating the immature dendritic cells with the cultivating medium supplemented with IL-4, GM-CSF, tumor necrosis factor alpha (TNF-), and Prostaglandin E.sub.2 (PGE.sub.2) to produce the mature dendritic cells. According to embodiments of the present disclosure, the PBMCs used in the present method are isolated from a leukocyte concentrate or a cryopreserved peripheral blood stem cells (PBSCs) stock.

Disclosed herein include methods, compositions, and kits suitable for use in preservation (e.g., cryopreservation) of samples. The composition (e.g., cryopreservation reagent) can comprise: one or more cryoprotective agent(s), one or more culture media component(s), one or more carbohydrate(s), and/or one or more amino acid(s). The composition can be capable of maintaining one or more cellular properties and/or cell viability of a plurality of cells in a sample for a period of time under a storage condition. Also provided are methods of sample preservation and sample analysis. The method can comprise contacting a sample with the cryopreservation reagent, thereby generating a shielded sample. The method can comprise exposing the shielded sample to a freezing storage condition, thereby generating a cryopreserved sample.

The present invention relates to a cryopreservation formulation comprising collagen hydrolysate and preferably comprising dimethylsulfoxide. The present furthermore relates to a method for cryopreservation of one or more biological materials, the method comprising the step of providing the one or more biological materials in the cryopreservation formulation comprising collagen hydrolysate and preferably dimethylsulfoxide. The present invention also relates to a use of the cryopreservation formulation comprising collagen hydrolysate and preferably dimethylsulfoxide for cryopreservation of one or more biological materials selected from the group consisting of an eukaryotic cell, a prokaryotic cell, a cell organelle, an extracellular vesicle, an organoid, a tissue, and an organ. The present invention also relates to a use of collagen hydrolysate, preferably in combination with dimethylsulfoxide, in preparing a cryopreservation formulation.

Disclosed herein include organelle complexes populations. The organelle complexes can comprise mitochondria and one or more of endoplasmic reticulum, peroxisomes, lysosomes, and Golgi apparatus. In some embodiments, the organelle complexes are isolated or derived from floating cells and/or frozen cells. In some embodiments, the organelle complexes are isolated or derived from cells contacted with a surfactant at a concentration at or above the critical micellar concentration (CMC) for the surfactant. At least about 80% of the mitochondria of the organelle complexes are capable of maintaining structural integrity in an extracellular environment. Also provided herein are methods for generating first organelle complexes populations.