An object of the present invention is to provide a method for preserving a platelet in a refrigerated or frozen state while retaining more platelet functions, a platelet preservation solution for use in such a method, and the like. The present invention relates to a platelet preservation solution for refrigeration or freezing, comprising an electrolyte and an anticoagulant. The present invention also relates to a method for preserving a platelet, comprising: suspending a platelet in the platelet preservation solution according to the present invention; and preserving the suspension by refrigeration or freezing at 10 C. or lower.

Described herein are methods, compositions, and kits for isolation of mitochondria from cryopreserved primary cells (e.g., fibroblast cells, mesenchymal stromal/stem cells) to be used in mitochondrial organelle transplantation (MOT) methods and compositions.

Provided herein are methods of producing and manufacturing iPSCs, such as iPSCs essentially free of exogeneous viral residuals, and RPE cells. Such methods may comprise cell seeding at low density (e.g., single-cell passaging) and/or culturing at elevated temperature.

The present disclosure relates to apoptosis-induced stem cells, a method for producing the same, and a pharmaceutical composition for the prevention or treatment of an inflammatory disease or a renal disease, including the same. Cells according to an aspect and a composition including the cells as an active ingredient have an increased IL-10 expression level without including a cryoprotectant, and thus can be effectively used for the prevention or treatment of an inflammatory disease or a renal disease.

The disclosure relates to composition and method for cryopreservation of mitochondria, cryopreserved composition comprising mitochondria and their therapeutic use.

Provided herein are methods and compositions relating to the use of anti-CD1 10 and anti-CD117 conditioning agents for depletion of endogenous hematopoietic stem cells in a subject, for example. prior to hematopoietic stem cell transplantation. Also provided are cell-based therapy methods and compositions.

Disclosed herein is a method for producing mature dendritic cells from peripheral blood mononuclear cells (PBMCs). The method includes the steps of, treating the PBMCs with a cultivating medium supplemented with interleukin 4 (IL-4) and granulocyte-macrophage colony-stimulating factor (GM-CSF) to produce immature dendritic cells; and then treating the immature dendritic cells with the cultivating medium supplemented with IL-4, GM-CSF, tumor necrosis factor alpha (TNF-), and Prostaglandin E.sub.2 (PGE.sub.2) to produce the mature dendritic cells. According to embodiments of the present disclosure, the PBMCs used in the present method are isolated from a leukocyte concentrate or a cryopreserved peripheral blood stem cells (PBSCs) stock.

Disclosed is a method of making and using a therapeutically potent cell for treating degenerative muscle disease. More specifically, disclosed is a method of making and using therapeutic cells, the method including identity and potency release assays for selecting an confirming therapeutic cells useful in ameliorating cardiac muscle and/or skeletal muscle degeneration associated with muscular dystrophy.

Disclosed herein include methods, compositions, and kits suitable for use in preservation (e.g., cryopreservation) of samples. The composition (e.g., cryopreservation reagent) can comprise: one or more cryoprotective agent(s), one or more culture media component(s), one or more carbohydrate(s), and/or one or more amino acid(s). The composition can be capable of maintaining one or more cellular properties and/or cell viability of a plurality of cells in a sample for a period of time under a storage condition. Also provided are methods of sample preservation and sample analysis. The method can comprise contacting a sample with the cryopreservation reagent, thereby generating a shielded sample. The method can comprise exposing the shielded sample to a freezing storage condition, thereby generating a cryopreserved sample.

A method for transporting a biological sample at hypothermic temperatures. The container can be cooled using phase change material and pump fluid through the sample. The fluid can pump through the system at a rate independent of the parameters of the biological sample. A valve can control the rate of flow of the fluid into the biological sample.