A method of controlling a blood analyzer for measuring platelets is provided. The method comprises: determining a relationship between at least one first measurement value obtained by detecting platelets in at least one previous test by an electrical type detector of the blood analyzer and at least one second measurement value obtained by detecting the platelets in the at least one previous test by an optical type detector of the blood analyzer, and controlling the blood analyzer to prepare the first and/or second measurement sample for a current test according to the determined relationship.

A method for measuring concentrations of blood cell components is provided. The method comprises: obtaining a blood sample from a subject, the blood sample comprising at least one of red blood cells (RBCs), white blood cells (WBCs), and platelets (PLTs); mixing the blood sample with a non-lysing aqueous solution to form a sample mixture comprising a predetermined tonicity; passing the sample mixture through a flow cell; emitting light towards the flow cell; measuring at least one of an amount of light absorbed by the RBCs to obtain an RBC absorption value, an amount of light scattered by WBCs to obtain a WBC scatter value, and an amount of light scattered by PLTs to obtain a PLT scatter value; and determining a concentration of at least one of the RBCs, WBCs, and PLTs present in the sample mixture.

A sample analyzer with an optical detection device and a sample analysis method of the sample analyzer are disclosed. The optical detection device includes a fluid chamber, a light source and a light detector. The fluid chamber includes an illumination zone. An analyte flows through the illumination zone so as to form a sample stream. The light source illuminates the illumination zone to excite cell articles, reacted with a reagent, of the sample stream to emit a light signal. The light detector detects the fluorescent lights and transforms it into an electric signal. The light detector can include a silicon photomultiplier.

Provided herein are systems and method for measuring cell stiffness. In particular, provided herein are microelectrode configuration and systems for measuring platelet deformation and stiffness.

Provided herein are systems and method for measuring cell stiffness. In particular, provided herein are microelectrode configuration and systems for measuring platelet deformation and stiffness.

In some instances, an apparatus can include a light sensitive imaging sensor having a surface to receive a fluid sample, a body to be moved relative to the light sensitive imaging sensor and having a surface to touch a portion of the fluid sample, and a carrier to move the body toward the surface of the light sensitive imaging sensor to cause the surface of the body to touch the portion of the fluid sample, so that as the surface of the body touches the portion of the fluid, the surface of the body (i) is parallel to the surface of the light sensitive imaging sensor, and (ii) settles on top of the fluid sample independently of motion of the carrier.

A blood cell analyzer comprises a flow cell configured to flow a measurement specimen containing blood cells, a first light source configured to emit light having a first wavelength, a second light source configured to emit light having a second wavelength different from the first wavelength, a first light receiving portion configured to receive first scattered light obtained by irradiating the blood cells passing through the flow cell with light from the first light source, a second light receiving portion configured to receive second scattered light obtained by irradiating the blood cells passing through the flow cell with light from the second light source, and a control section configured to discriminate at least red blood cells from the blood cells contained in the measurement specimen based on detection signals output from the first light receiving portion and the second light receiving portion, respectively.

A sample measuring apparatus of an embodiment includes: a laser diode that applies laser light to a measurement specimen prepared from a sample; a detection unit that acquires optical information from a particle in the measurement specimen to which the laser light is applied; a drive circuit that supplies a direct-current drive signal to the laser diode; and a high-frequency conversion circuit that generates a potential that switches between a high level and a low level in a predetermined cycle to guide the drive signal outputted from the drive circuit to a second signal path which is different from a first signal path connected to the laser diode in the predetermined cycle, thereby converting the drive signal to be supplied to the laser diode into a high-frequency signal.

The invention, provides a method, apparatus and system for separating blood and other types of cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing. One of the exemplary methods includes providing a first flow having a plurality of blood components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first blood cellular component of the plurality of blood components into the second flow while concurrently maintaining a second blood cellular component of the plurality of blood components in the first flow. The second flow having the first blood cellular component is then differentially removed from the first flow having the second blood cellular component. Holographic optical traps may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage.

An optical detection system, a blood cell analyzer and a platelet detection method are provided. The optical detection system includes: an optical subsystem, a flow chamber and a first detector; the optical subsystem includes a laser, a front optical assembly including an optical isolator, and a rear optical assembly including a blocking diaphragm. The laser is configured to emit a laser beam; the front optical assembly is configured to perform front optical treatment; the rear optical assembly is disposed downstream of the flow chamber in the propagation direction of the laser beam, and is configured to perform rear optical treatment on the scattered light and the laser beam converged at the blocking diaphragm; and the optical isolator is configured to isolate reflected light that is generated when the laser beam passes through the flow chamber.