The invention relates to a formulation for total and differential counting of leukocytes for use in clinical analyses, in particular hemograms, to promote instantaneous differential staining of leukocytes in liquid medium, allowing greater practicality and agility in the total and differential counting of leukocytes. The practicality and agility are related to the use of a single dye for two parameters, eliminating the need for a smear. It results in a satisfactory effect for staining cells in suspension, staining the nucleus and cytoplasm of leukocytes in different shades, allowing their visualization under an optical microscope or in Point-of-Care image processing devices. It uses easily accessible raw materials, including cresyl acetate violet, ethyl alcohol, sodium and potassium chlorides, sodium hydroxide, acetic acid, triton X and deionized water, presenting a simple manufacturing process and low cost.

Disclosed are a sample analysis apparatus and method which include: obtaining a first light signal by irradiating a first test sample from blood and a first-channel reagent to differentiate neutrophils, eosinophils, lymphocytes, and monocytes; obtaining a second light signal from a second test sample from the blood and a second-channel reagent; counting the nucleated red blood cells and lymphocytes based on the second light signal; determining accuracy of the lymphocyte result from the first test sample; and if inaccurate, correcting the lymphocyte result based on the lymphocyte result of the second test sample.

A cell analyzer and a particle sorting method and device are disclosed. The method comprises: acquiring a pulse width of at least one optical signal according to a detected optical signal, selecting at least one optical signal as a combined optical signal, and respectively calculating a signal intensity of the combined optical signal with the pulse width in a combinatorial way to obtain at least one reinforcement signal, where a difference between a first category of particles and a second categories of particles in the reinforcement signal is increased relative to a difference therebetween in the combined optical signal; and on the basis of the reinforcement signal and at least another signal, forming a new scatter diagram, where the at least another signal is one of other reinforcement signals and the optical signal, distinguishing the first category of particles from the second category of particles according to the new scatter diagram.

Disclosed in one aspect is a method for performing a complete blood count (CBC) on a sample of whole blood by metering a predetermined amount of the whole blood and mixing it with a predetermined amount of diluent and stain and transferring a portion thereof to an imaging chamber of fixed dimensions and utilizing an automated microscope with digital camera and cell counting and recognition software to count every white blood cell and red blood corpuscle and platelet in the sample diluent/stain mixture to determine the number of red cells, white cells, and platelets per unit volume, and analyzing the white cells with cell recognition software to classify them.

Devices for sorting components (e.g., cells) contained in a liquid sample are provided. In certain aspects, the devices include a magnetic separation device and an acoustic concentrator device fluidically coupled to magnetic separation device. Aspects of the invention further include methods for sorting cells in a liquid sample, and systems, and kits for practicing the subject methods.

A urine specimen analysis device includes a specimen drawing portion, a sample preparing portion, a measurement portion, and an information processing portion. The specimen drawing portion draws a first aliquot and a second aliquot from a urine specimen. The sample preparing portion prepares a first measurement sample by mixing the first aliquot and a first staining dye that stains red blood cells, and a second measurement sample by mixing the second aliquot and a second staining dye that stains nucleic acids. The measurement portion measures fluorescence emitted from the first measurement sample prepared by the sample preparing portion, and fluorescence emitted from the second measurement sample prepared by the sample preparing portion. The information processing portion detects at least red blood cells contained in the first measurement sample based on the fluorescence of the first measurement sample measured by the measurement portion, and at least white blood cells contained in the second measurement sample based on the fluorescence of the second measurement sample measured by the measurement portion.

Apparatuses and methods for analyzing fluid samples are provided. For example, an example apparatus may include a fluid imaging chamber, at least one illumination source component, and an image sensor component. In some examples, the fluid imaging chamber comprises a flow channel for receiving a fluid sample. In some examples, the at least one illumination source component is configured to emit at least one light beam, and the at least one light beam is directed through the fluid sample in the flow channel from a top surface of the fluid imaging chamber. In some embodiments, the image sensor component is positioned under a bottom surface of the fluid imaging chamber and configured to generate digital holography image data of the fluid sample.

A specimen analyzing method of an embodiment includes: preparing a measurement specimen from a biological specimen containing blood cells by staining, with a nucleic acid staining fluorescent dye, nucleic acids contained in neutrophils not having released neutrophil extracellular traps; obtaining optical information including fluorescence information by irradiating the measurement specimen with light; and detecting, as neutrophil extracellular traps on the basis of the optical information, particles having lower fluorescence intensity than fluorescence intensity obtained from the neutrophils not having released neutrophil extracellular traps.

A method for measuring concentrations of blood cell components is provided. The method comprises: obtaining a blood sample from a subject, the blood sample comprising at least one of red blood cells (RBCs), white blood cells (WBCs), and platelets (PLTs); mixing the blood sample with a non-lysing aqueous solution to form a sample mixture comprising a predetermined tonicity; passing the sample mixture through a flow cell; emitting light towards the flow cell; measuring at least one of an amount of light absorbed by the RBCs to obtain an RBC absorption value, an amount of light scattered by WBCs to obtain a WBC scatter value, and an amount of light scattered by PLTs to obtain a PLT scatter value; and determining a concentration of at least one of the RBCs, WBCs, and PLTs present in the sample mixture.

An exemplary embodiment of the present disclosure provides a live cell imaging system, comprising a substrate, a UV light source, and a UV camera. The substrate can have a cavity configured to hold a sample. The sample can comprise one or more live cells. The substrate can be made, at least in part, out of polydimethylsiloxane (PDMS). The UV light source can be configured to direct UV light to the sample. The UV camera can be configured to take a UV image of the sample.