A particle separating device includes at least three liquid chambers adapted to store a liquid therein; at least two liquid passages, each connecting adjacent two of the liquid chambers; an inlet adapted to introduce a liquid in which multiple particles of different sizes are dispersed into one of the liquid chambers; and at least two electrodes disposed inside at least two of the liquid chambers, respectively, the electrodes adapted to apply different electrical potentials to the liquid. The cross-sectional areas of the at least two liquid passages are different from each other.

A particle analysis device includes multiple stacked plates joined together; an upper liquid space adapted to store a first liquid; a lower liquid space adapted to store a second liquid; a connection pore connecting the upper liquid space to the lower liquid space; a first hole extending from the top surface to the upper liquid space, the first liquid flowing through the first hole; and a second hole extending from the top surface to the lower liquid space, the second liquid flowing through the second hole. A first electrode and a second electrode that are sheets are pinched between two of the plates. The first electrode applies an electric potential to the first liquid in the upper liquid space through the first hole, whereas the second electrode applies an electric potential to the second liquid in the lower liquid space through the second hole. The particle analysis device further includes a first electrode-rod-insertion hole extending from the top surface to the first electrode, and a second electrode-rod-insertion hole extending from the top surface to the second electrode. The first electrode and the second electrode are not exposed at any side surface of the particle analysis device.

A particle analysis device includes a liquid space adapted to store a liquid; a chip disposed above the liquid space, the chip having a connection pore extending vertically and communicating with the liquid space; an upper hole disposed above the chip, the upper hole extending vertically and communicating with the connection pore; a first electrode adapted to apply an electric potential to a liquid in the upper hole; and a second electrode adapted to apply an electric potential to the liquid in the liquid space. The upper hole having a diameter that is equal to or greater than the maximum width of the connection pore, and the entirety of the connection pore falling within the range of the upper hole.

A system and method for electrical label-free detection of single protein molecules via a nanoscale electrode based on detecting the transient potential change of the floating nanoelectrode, which works for both large and small molecules. The system can also be applied to study the interactions of molecules with molecular receptors on the surface of the nanoscale electrode. The motion and dynamics of the protein near the nanoscale electrode can be detected with high precision in real time based on their intrinsic charges by the potentiometric method using a differential amplifier. The nanoelectrode can be integrated into a microfluidic device for biosensing applications.

A number analyzing method, a number analyzing device, and a storage medium for number analysis are disclosed, which enable, with high accuracy, analysis of the number or number distribution of particulate or molecular analytes according to the kinds of the analytes. A computer control program is executed on the basis of a data group of particle-passage detection signals which are detected by a nanopore device in accordance with passage of subject particles through a through-hole. Also, a particle type distribution estimating program is executed, to estimate probability density on the basis of a data group based on feature values indicating feature of the waveforms of pulse signals which correspond to the passage of particles and which are obtained as the particle-passage detection signals. Thus, the number of particles can be derived for each particle type.

A method having the steps of obtaining prepared image data captured by an image sensor receiving light propagated across a sample volume, containing a fluid possibly comprising microscopic objects of foreign origin, while illuminating the sample volume by coherent light. The prepared image data comprising, for a microscopic object, a prepared hologram pattern with prepared spatially alternating intensity formed by the interference fringes; providing filtered image data, comprising automatically filtering the prepared image data by an edge enhancing filter. the filtered image data comprising, for a prepared hologram pattern, a filtered hologram pattern. The presence of the microscopic object associated with the filtered hologram pattern in the sample volume of the fluid is automatically detected on the basis of the filtered hologram pattern.

A number analyzing method, a number analyzing device, and a storage medium for number analysis are disclosed, which enable, with high accuracy, analysis of the number or number distribution of particulate or molecular analytes according to the kinds of the analytes. A computer control program is executed on the basis of a data group of particle-passage detection signals which are detected by a nanopore device in accordance with passage of subject particles through a through-hole. Also, a particle type distribution estimating program is executed, to estimate probability density on the basis of a data group based on feature values indicating feature of the waveforms of pulse signals which correspond to the passage of particles and which are obtained as the particle-passage detection signals. Thus, the number of particles can be derived for each particle type.

Various implementations, systems and methods are disclosed for continuous, near real-time, hands-off sampling of airborne particulate matter, and qualification and/or quantification of biomolecules in the sample representative for biologic or microbial contamination. The systems and methods may utilize an electrostatic precipitator for sampling the matter; and a measurement assembly configured to illuminate, excite, or breakdown the sampled matter by electromagnetic radiation, and to detect a spectrum, or one or more wavelength bands of the scatter emitted by the sample. In an exemplary implementation, a sputter deposition process is employed to configure the sample for an enhanced plasmon resonance. The measurement data may be transferred via wireless communication means for cloud storage and signal processing.

A sensor for particle identification, the sensor comprising: a first chamber configured to be filled with an electrolytic solution; a first electrode provided inside the first chamber and configured to be connected to an external power supply for applying a voltage; a second chamber configured to be filled with the electrolytic solution; a second electrode provided inside the second chamber and configured to be connected to the external power supply; a data output means configured to output measurement data expressing an ion current generated between the first electrode and the second electrode; a partition separating the first chamber and the second chamber; and a presentation means for providing a unique identifier to an external computer device over a network. The partition includes a pore connecting the first chamber and the second chamber, a physical property of the sensor is associated with the unique identifier, the sensor is configured such that when a particle passes through the pore, a transient change dependent on at least a physical property of the pore and a physical property of the particle occurs in the ion current generated between the first electrode and the second electrode, and the unique identifier is configured to cause the external computer device receiving the unique identifier to perform a process of identifying the particle according to the physical property of the sensor associated with the unique identifier. The physical property of the sensor at least includes a physical property of the pore.

The present invention describes a fluidic device for measuring at least one of corpuscle mass density and weight. The fluidic device comprises a sedimentation chamber fluidly connected to an inlet channel configured to be immersed in a liquid. The fluidic device further comprises a pumping system connected to the sedimentation chamber. The pumping system is adapted to control the flow of liquid in the sedimentation chamber. A processor of the fluidic device is configured to obtain corpuscle data related to a corpuscle in at least one region of the sedimentation chamber; and calculate at least one of corpuscle mass density and weight based on the data received.