An apparatus including a fluidic input and a die including a microfluidic chamber, may receive a biologic sample. The microfluidic chamber may include a foyer to contain a portion of the biologic sample, and an inlet impedance-based sensor to detect passage of a cell of the biologic sample into the foyer. A target nozzle may eject a first volume, corresponding with a target concentration of cells of the biologic sample. A spittoon nozzle may eject a second volume of the portion of the biologic sample into a spittoon location. An output impedance-based sensor may be disposed within a threshold distance of the target nozzle to detect passage of a cell of the biologic sample into the target nozzle. Moreover, the apparatus may include circuitry to control firing of the target nozzle and the spittoon nozzle based on signals received from the inlet impedance-based sensor and the output impedance-based sensor.

The present invention describes a fluidic device for measuring at least one of corpuscle mass density and weight. The fluidic device comprises a sedimentation chamber fluidly connected to an inlet channel configured to be immersed in a liquid. The fluidic device further comprises a pumping system connected to the sedimentation chamber. The pumping system is adapted to control the flow of liquid in the sedimentation chamber. A processor of the fluidic device is configured to obtain corpuscle data related to a corpuscle in at least one region of the sedimentation chamber; and calculate at least one of corpuscle mass density and weight based on the data received.

An example system includes an input channel having a first end and a second end to receive particles through the first end, a sensor to categorize particles in the input channel into one of at least two categories, and at least two output channels Each output channel is coupled to the second end of the input channel to receive particles from the input channel, and each output channel is associated with at least one category of the at least two categories. Each output channel has a corresponding pump operable, based on the categorization of a detected particle in a category associated with a different output channel, to selectively slow, stop, or reverse a flow of particles into the output channel from the input channel.

Some embodiments of the methods provided herein relate to sample analysis and particle characterization methods. Some such embodiments include receiving, from a particle analyzer, measurements for a first portion of particles associated with an experiment. Some embodiments also include generating a tree representing groups of related particles based at least in part on the measurements, wherein the tree includes at least three groups. Some embodiments also include generating a measure of relatedness between a first group and a second group of the tree based at least in part on the measurements. Some embodiments also include and configuring the particle analyzer to classify a subsequent particle associated with the experiment with the first group real-time, wherein the subsequent particle is not included in the first portion of particles. Some embodiments also include sorting the subsequent particle.

A particle separating apparatus and method are provided, which pass a fluid sample such as blood through a filter to remove foreign matter, and separate target particles by using a MOFF channel, and re-separate the separated target particles through dielectrophoresis. The particle separating apparatus includes a MOFF (Multi Orifice Flow Fractionation) channel including a multi orifice segment through which a fluid sample passes to discharge a primarily separated material that are target particles separated from the fluid sample, through a central passage; a dielectrophoresis channel including a pair of electrodes to which AC power is applied and forming an electric field in a flow channel connected to the central passage of the MOFF channel to re-separate the target particles from the primarily separated material discharged from the central passage of the MOFF channel through dielectrophoresis.

A microfluidic sorting device and method employing dielectrophoresis (DEP) induced field flow separations are described herein. The microfluidic sorting device has a microchannel and an array of electrodes disposed along the microchannel. The electrodes may be oriented at an angle relative to the microchannel. Non-mammalian samples such as plant samples flow in the microchannel and through the electrode array. Current is passed through the electrodes causing a DEP force to be exerted on the samples. This force may generate a torque that causes one type of sample to rotate and slide along the electrodes, thus separating the samples by type. The separated samples are collected in different output channels

Embodiments for sorting particles are provided that include a microfluidic channel configured to receive a microfluidic flow that comprises a plurality of particles having different characteristics, the microfluidic channel having a plurality of output flow channels, a first detector configured to detect the location of the particles, a plurality of actuators located along the direction of the microfluidic flow and defining a sorting electrode arrangement. The microfluidic device further comprises a controller configured to receive signals from the first detector and to provide force field profiles for each of the plurality of particles, wherein each force field profile comprises a plurality of deflection force settings along the direction of the microfluidic flow. The controller individually addresses the plurality of actuators to generate a plurality of actuation inducing fields along the direction of the microfluidic flow to generate the deflection force settings in the force field profiles.

This application discloses methods for assessing quantities of spherical or substantially spherical lipoprotein particles or portions thereof present in a biological sample based on the measurement of free cholesterol and/or phospholipid content in the lipoprotein particles. Methods of treating a subject at increased risk for cardiovascular disease and/or cardiodiabetes are also disclosed.

Disclosed herein is a system to detect and characterize individual particles and cells using at least either optic or electric detection as the particle or cell flows through a microfluidic channel. The system also provides for sorting particles and cells or isolating individual particles and cells.

A microchannel for processing cells by compression of the cells including an inlet, ridges and an outlet. Each ridge including a compressive surface and a cell adhesion entity. The outlet configured to remove at least one of a first portion of the cells and a second portion of the cells from the microchannel. Each ridge oriented at an angle of from 25 degrees to 70 degrees relative to a center axis of the microchannel. The cell adhesion entity configured such that the first portion of the cells has a first adhesion property relative to the cell adhesion entity to follow a first trajectory through the microchannel. The cell adhesion entity further configured such that the second portion of the cells has a second adhesion property relative to the cell adhesion entity to follow a second trajectory through the microchannel. The first trajectory is different from the second trajectory.