An imaging system having multiple cameras providing a large field of view with sufficient resolution can be used for tracking movements of cells from their positions in a tissue sample into multiple isolated areas such as into individual microwells in a well plate. By determining the beginning and the end of the movements of each cell, the imaging system can associate the microwell locations to the original cell positions in the sample. Together with an analysis of the cells in the microwells, either individually or together with barcode beads, the analysis can achieve the spatial information needed for constructing a map of the molecular information with respect to the positions of the cells in the sample.

Among other things, a method comprises imaging a sample displaced between a sensor surface and a surface of a microscopy sample chamber to produce an image of at least a part of the sample. The image is produced using lensless optical microscopy, and the sample contains at least blood from a subject. The method also comprises automatically differentiating cells of different types in the image, generating a count of one or more cell types based on the automatic differentiation, and deriving a radiation dose the subject has absorbed based on the count.

Systems and methods for analyzing blood samples, and more specifically for performing a nucleated red blood cell (nRBC) analysis. The systems and methods screen a blood sample by means of fluorescence staining and a fluorescence triggering strategy, to identify nuclei-containing particles within the blood sample. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder reagent(s), suitable for assays of samples containing fragile white blood cells (WBCs). In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; (b) using a fluorescence trigger to screen the blood sample for nuclei-containing particles; and (c) using measurements of light scatter and fluorescence emission to distinguish nRBCs from WBCs.

Provided are methods for quantifying and/or detecting sub-visible particulates in cell cultures. Specifically, the methods comprise a step of breaking down, e.g., lysing, cells in a cell culture. The methods can further comprising filtering the cell culture through a filter. Further provided are methods of quantifying sub-visible particulates that do not pass through the filter using a microscope.

Provided herein are systems and methods for analyzing blood samples, and more specifically for performing a basophil analysis. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; and then (b) using measurements of light scatter and fluorescence emission to distinguish basophils from other WBC sub-populations. In one embodiment, the systems and methods include performing a basophil cluster analysis of the blood sample, based on the combination of light scatter and fluorescence measurements.

The present invention relates generally to an apparatus for identifying biological particles. More particularly, the present invention relates to a small apparatus for identifying biological particles, wherein in a single apparatus having a simple structure, a cleaning solution is suctioned to separate the biological particles from a filter and a sample solution is discharged, the discharged sample solution is injected into a plurality of ticket modules, and the biological particles are identified by image analysis for the ticket modules, thereby enabling miniaturization of the apparatus.

A system and process may be used to test water samples to measure ATP and/or estimate a microbial population, for example using an adenosine triphosphate (ATP) based assay. The system includes a device that is use in combination with single-use or disposable cartridges. The cartridge receives the water sample and is pre-loaded with one or more reagents. The device receives the cartridge and contains physical, electronic and/or mechatronic devices that interact with cartridge. One or more actions such as metering, mixing and conveying are performed automatically by elements of the device and/or cartridge. A sensor in the device measures light produced in the cartridge from a reaction with ATP in the water sample. Optionally, the cartridge also contains a pre-loaded amount of ATP, which is used to provide an internal reference or calibration measurement.

A particle measuring device has an upper surface having a first flow inlet to receive a first fluid containing target particles to be measured, a first flow path connected to the first flow inlet to allow measurement of the target particles, and a third flow path located upstream from and connected to a joint between the first flow path and the first flow inlet and having a smaller width than the first flow inlet. The first flow path includes a first planar portion having a greater width than the third flow path and the first flow inlet, a width-increasing portion located downstream from and connected to the first planar portion, and a second planar portion located downstream from and connected to the width-increasing portion.

Devices and methods are described for measuring formed blood component sedimentation rate. Some of the methods may use (1) centrifugal techniques for separating red blood cells from plasma and (2) video and/or still imaging capability. Both may be used alone or in combination to accelerate formed blood component sedimentation and to measure its rate. In one example, the method may advantageously enable rapid measurement of sedimentation rate using small blood sample volumes. Automated image analysis can be used to determine both sedimentation rate and hematocrit. Automated techniques may be used to compensate for effects of hematocrit on uncorrected sedimentation rate data.

In one example in accordance with the present disclosure, a cellular analytic system is described. The cellular analytic system includes an analytic device. The analytic device includes a chamber to receive a cell to be analyzed. At least one lysing element agitates the cell and at least one sensor detects a change in the cell based on an agitation of the cell. The cellular analytic system also includes a controller to determine a rupture threshold of the cell based on parameters of the agitation when a cell membrane ruptures.